Tumor Mesenchymal Stromal Cells Regulate Cell Migration of Atypical Teratoid Rhabdoid Tumor through Exosome-Mediated miR155/SMARCA4 Pathway

Yang, Yi; Nguyen, Phan Nguyen Nhi; Hsin, I.; Ho, Wen Jin; Chen, Yi Wei; Chien, Yueh; Yarmishyn, Aliaksandr A.; Huang, Pin I.; Lo, Wen-Liang; Wang, Chien Ying; Liu, Yung Yang; Lee, Yi Yen; Lin, Chien Min; Chen, Ming Teh; Wang, Mong‐Lien

doi:10.3390/cancers11050720

Cited by 23 publications

(16 citation statements)

References 55 publications

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“…Furthermore, miR-155 directly targeted and inhibited a series of genes (e.g. FOXO3, SMARCA4 or Ubiquilin-1) to participate in the biological process of tumor development (37)(38)(39)(40). To the best of our knowledge, the present study was the first to indicate that TERF1 may be a direct target of miR-155, as confirmed by a dual luciferase reporter assay.…”

Section: Discussionsupporting

confidence: 65%

TERF1 downregulation promotes the migration and invasion of the PC3 prostate cancer cell line as a target of miR‑155

Chen

He²,

Liang

et al. 2020

Mol Med Rep

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Telomeric repeat binding factor 1 (TerF1) has been identified as a tumor suppressor gene in numerous types of human cancer. However, the expression of TERF1 and its mechanism in prostate cancer (PCa) remains unclear. The present study aimed to explore the expression and functions of TERF1 in PCa. The UALCAN database was used to analyze the differential expression of TERF1 between normal prostate tissue and primary PCa tissue. Cell apoptosis was analyzed by Annexin V/propidium iodide staining, and wound healing and Transwell assays were used to detect the cell migration and invasion abilities, respectively. The cell viability was analyzed using an MTT assay. Reverse transcription-quantitative PCR and western blotting were used to analyze the mRNA and protein expression levels, respectively, of epithelial-mesenchymal transition (EMT) markers following TERF1 knockdown in the PC3 cell line. A dual luciferase reporter assay was used to verify the association between TERF1 and microRNA (miR)-155 predicted by bioinformatics analysis. Rescue experiments were performed to determine the role of the miR-155/TERF1 axis in regulating the cellular behaviors of PCa. The results demonstrated that the expression levels of TERF1 in the primary prostate tumors were significantly downregulated compared with in prostate normal tissue. TERF1 silencing was discovered to significantly promote cell viability, migration and invasion, while suppressing cell apoptosis. The impact of TERF1 on PC3 cells was suggested to occur through the EMT pathway. TERF1 was confirmed to be the direct target of miR-155. The overexpression of miR-155 promoted the viability, migration and invasion, while suppressing the apoptosis of the PC3 cell line, while the knockdown of miR-155 in PC3 cells achieved the opposite trends. In addition, TERF1 overexpression reversed the promotive effects of upregulated miR-155 expression levels on the migration and apoptosis of PC3 cells. On the contrary, the knockdown of TERF1 reversed the migration and apoptosis abilities of the downregulated miR-155 expression levels on the cellular behaviors of PC3 cells. In conclusion, TERF1, as a direct target of miR-155, was discovered to be significantly downregulated in PCa, which was suggested to promote the migration and invasion of PCa via the EMT pathway.

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Section: Discussionsupporting

confidence: 65%

TERF1 downregulation promotes the migration and invasion of the PC3 prostate cancer cell line as a target of miR‑155

Chen

He²,

Liang

et al. 2020

Mol Med Rep

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“…91 Exosomes from tumor-associated MSCs contained high levels of miR-155, which, after being taken up by atypical teratoid/rhabdoid tumor cells, could cause tumor suppressor genes SMARCA4 (direct target genes of miR-155) inhibition, and enhanced atypical teratoid/ rhabdoid tumor migration ability. 92 In addition, glioma-associated human MSCs, a potential new matrix component in glioma, could drive the invasibility of glioma stem cells (GSCs) and have been identified as a new therapeutic target in glioma. 93 miR-1587 in glioma-associated human MSCs derived exosomes promoted proliferation and clonal formation in GSCs by down-regulating the tumor-suppressive nuclear receptor corepressor NCOR1 in GSCs.…”

Section: Exosome and Cancer-associated Fibroblastsmentioning

confidence: 99%

Exosomes: key players in cancer and potential therapeutic strategy

Dai

Zhong

et al. 2020

Sig Transduct Target Ther

796

504

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Exosomes are extracellular vesicles secreted by most eukaryotic cells and participate in intercellular communication. The components of exosomes, including proteins, DNA, mRNA, microRNA, long noncoding RNA, circular RNA, etc., which play a crucial role in regulating tumor growth, metastasis, and angiogenesis in the process of cancer development, and can be used as a prognostic marker and/or grading basis for tumor patients. Hereby, we mainly summarized as followed: the role of exosome contents in cancer, focusing on proteins and noncoding RNA; the interaction between exosomes and tumor microenvironment; the mechanisms that epithelial-mesenchymal transition, invasion and migration of tumor affected by exosomes; and tumor suppression strategies based on exosomes. Finally, the application potential of exosomes in clinical tumor diagnosis and therapy is prospected, which providing theoretical supports for using exosomes to serve precise tumor treatment in the clinic.

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“…Multiple different molecular structures have been identified in the liquids of pediatric brain tumor patients and raised the interest of the scientific field. These biomarkers include circulating tumor cells (CTCs) [ 18 , 19 ], circulating tumor DNA (ctDNA) [ 7 , 18 , 19 ], cell-free DNA (cfDNA) [ 6 , 18 , 19 , 20 , 21 ], circulating proteins [ 6 , 8 , 19 , 20 ], extracellular vesicles and exosomes [ 5 , 18 , 22 , 23 , 24 , 25 , 26 ], micro-RNAs (miRNAs) [ 5 , 19 , 27 , 28 , 29 , 30 , 31 , 32 ], long non-coding RNA (lncRNA) [ 33 ] and other genetic alterations [ 28 , 34 , 35 , 36 , 37 , 38 ]. Detection of these biomolecules may offer advantages compared to surgical interventions.…”

Section: Introductionmentioning

confidence: 99%

Liquid Biomarkers for Pediatric Brain Tumors: Biological Features, Advantages and Perspectives

Madlener

Gojo

2020

JPM

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Tumors of the central nervous system are the most frequent solid tumor type and the major cause for cancer-related mortality in children and adolescents. These tumors are biologically highly heterogeneous and comprise various different entities. Molecular diagnostics are already well-established for pediatric brain tumors and have facilitated a more accurate patient stratification. The availability of targeted, biomarker-driven therapies has increased the necessity of longitudinal monitoring of molecular alterations within tumors for precision medicine-guided therapy. Nevertheless, diagnosis is still primarily based on analyses of the primary tumor and follow-up is usually performed by imaging techniques which lack important information on tumor biology possibly changing the course of the disease. To overcome this shortage of longitudinal information, liquid biopsy has emerged as a promising diagnostic tool representing a less-invasive source of biomarkers for tumor monitoring and therapeutic decision making. Novel ultrasensitive methods for detection of allele variants, genetic alterations with low abundance, have been developed and are promising tools for establishing and integrating liquid biopsy techniques into clinical routine. Pediatric brain tumors harbor multiple molecular alterations with the potential to be used as liquid biomarkers. Consequently, studies have already investigated different types of biomarker in diverse entities of pediatric brain tumors. However, there are still certain pitfalls until liquid biomarkers can be unleashed and implemented into routine clinical care. Within this review, we summarize current knowledge on liquid biopsy markers and technologies in pediatric brain tumors, their advantages and drawbacks, as well as future potential biomarkers and perspectives with respect to clinical implementation in patient care.

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Tumor Mesenchymal Stromal Cells Regulate Cell Migration of Atypical Teratoid Rhabdoid Tumor through Exosome-Mediated miR155/SMARCA4 Pathway

Cited by 23 publications

References 55 publications

TERF1 downregulation promotes the migration and invasion of the PC3 prostate cancer cell line as a target of miR‑155

TERF1 downregulation promotes the migration and invasion of the PC3 prostate cancer cell line as a target of miR‑155

Exosomes: key players in cancer and potential therapeutic strategy

Liquid Biomarkers for Pediatric Brain Tumors: Biological Features, Advantages and Perspectives

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