Tumor microenvironment-targeted poly-L-glutamic acid-based combination conjugate for enhanced triple negative breast cancer treatment

Characterization of triple‐negative breast cancer preclinical models provides functional evidence of metastatic progression

“…Therefore, high CAA content of MDA‐MB‐231‐Luc tumors may serve as a faithful model to study this phenomenon and develop treatments to avoid adipocytic protumorigenic effects. Furthermore, high‐lipid content and low‐cellular density in the tumor stroma generated a softer tumor mass with a threefold higher EPR effect, thereby providing a model to assess EPR‐related passive tumor accumulation, especially important for the effectiveness of nanomedicinal approaches to cancer treatment . The flexibility and permeability of the MDA‐MB‐231‐Luc model stroma may also provide a means to assess intratumoral administration strategies …”

“…Furthermore, high-lipid content and low-cellular density in the tumor stroma generated a softer tumor mass with a threefold higher EPR effect, thereby providing a model to assess EPR-related passive tumor accumulation, especially important for the effectiveness of nanomedicinal approaches to cancer treatment. 7,30,31 The flexibility and permeability of the MDA-MB-231-Luc model stroma may also provide a means to assess intratumoral administration strategies. 32 The metabolomic comparison of 4T1 and MDA-MB-231-Luc primary tumors suggests that specific metabolic alterations could explain the differences observed in growth rates, with guanosine and uracil (a pyrimidine derivative) the two most relevant soluble metabolites contributing to model discrimination.…”

Polyglutamic acid-based crosslinked doxorubicin nanogels as an anti-metastatic treatment for triple negative breast cancer

“…47 We inoculated animals in the mammary fat pad with 4T1 cells following previously optimized protocols. 48,49 Treatments began at day 8, a time point that coincided with the maximum enhanced permeability and retention effect 50 when tumors sizes had reached ~0.1 cm 3 , to promote efficient tumor uptake. After intravenous injection through the tail vein (three doses every three days), we monitored tumor growth for 16 days.…”

The generation of stabilized supramolecular nanorods from star-shaped polyglutamates

“…[5][6][7][8] Among the described biocompatible polypeptides, poly-L-glutamates (PGA) display low immunogenicity, high biodegradability, and controlled biodistribution profiles, making them a focus for research. 9,10 Many studies have demonstrated that physicochemical properties strongly influence the in vivo behavior of particles. 8 The role of charge, size, and surface decoration have been described in detail; however, the role of shape has remained mostly unstudied.…”