Tumor necrosis factor-alpha concentration in the aqueous humor of healthy and diseased dogs: A preliminary pilot study

Durieux, P; Etchepareborde, S.; Fritz, Darko; Rosolen, Serge G.

doi:10.1016/j.jfo.2014.10.014

Cited by 7 publications

(8 citation statements)

References 16 publications

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“…176 TNFa is also upregulated in the aqueous humor in humans and dogs with glaucoma. 177,178 TNFa is upregulated in the vitreous in glaucomatous human patients and TNFa experimentally injected in the vitreous in normal mice can induce the degenerative changes characteristic of GON, without increasing the IOP. 179 Increased TNFa levels activate microglial cells, the retinal and optic nerve macrophages, which produce additional amounts of TNFa, disrupting the homeostatic balance and starting a vicious cycle.…”

Section: Activation Of Glial Cellsmentioning

confidence: 99%

Definition, Classification, and Pathophysiology of Canine Glaucoma

Pizzirani

2015

Veterinary Clinics of North America: Small Animal Practice

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Section: Activation Of Glial Cellsmentioning

confidence: 99%

Definition, Classification, and Pathophysiology of Canine Glaucoma

Pizzirani

2015

Veterinary Clinics of North America: Small Animal Practice

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“…Modulation of tumor necrosis factor–α (TNF-α) and its receptors is correlated to the development of glaucoma, with increased immunoreactivity in glaucomatous canine retinas. 47 Significantly higher TNF-α levels were detected in the aqueous humor of all dogs with intraocular disease compared with dogs with normal eyes, 26 and TNF-α levels were significantly higher in those with acute anterior uveitis compared with those with PACG. 26…”

Section: Biochemical Markers In Canine Glaucomamentioning

confidence: 92%

Genetic and Biochemical Biomarkers in Canine Glaucoma

2016

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In many health-related fields, there is great interest in the identification of biomarkers that distinguish diseased from healthy individuals. In addition to identifying the diseased state, biomarkers have potential use in predicting disease risk, monitoring disease progression, evaluating treatment efficacy, and informing pathogenesis. This review details the genetic and biochemical markers associated with canine primary glaucoma. While there are numerous molecular markers (biochemical and genetic) associated with glaucoma in dogs, there is no ideal biomarker that allows early diagnosis and/or identification of disease progression. Genetic mutations associated with canine glaucoma include those affecting ADAMTS10, ADAMTS17, Myocilin, Nebulin, COL1A2, RAB22A, and SRBD1. With the exception of Myocilin, there is very limited crossover in genetic biomarkers identified between human and canine glaucomas. Mutations associated with canine glaucoma vary between and within canine breeds, and gene discoveries therefore have limited overall effects as a screening tool in the general canine population. Biochemical markers of glaucoma include indicators of inflammation, oxidative stress, serum autoantibodies, matrix metalloproteinases, tumor necrosis factor-α, and transforming growth factor-β. These markers include those that indicate an adaptive or protective response, as well as those that reflect the damage arising from oxidative stress.

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“…Paracentesis‐induced anterior uveitis in dogs is an acute anterior uveitis model characterized by BAB breakdown . The symptoms are relatively mild compared with those of clinical cases . Prostaglandin E 2 (PGE 2 ) is considered a major mediator involved in BAB breakdown in this model .…”

Section: Introductionmentioning

confidence: 95%

“…7 The symptoms are relatively mild compared with those of clinical cases. 8 Prostaglandin E 2 (PGE 2 ) is considered a major mediator involved in BAB breakdown in this model. 9 Therefore, systemic or topical administration of corticosteroids and nonsteroidal anti-inflammatory drugs (NSAIDs), inhibitors of PG synthesis, before paracentesis has been reported to greatly prevent increased BAB permeability in dogs.…”

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confidence: 99%

Comparison of early posttreatment effects of two steroidal anti‐inflammatory ophthalmic drugs on the ocular inflammatory response induced by paracentesis in healthy canine eyes

Sato

Iwasaki

Nagai

et al. 2019

Veterinary Ophthalmology

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Objective We investigated the early posttreatment effects of two steroidal anti‐inflammatory ophthalmic drugs on blood‐aqueous barrier (BAB) breakdown by paracentesis in dogs. Animal studies We studied 21 healthy beagles with normal eyes. Procedures Controlled anterior chamber paracentesis (0.5 mL) was performed in one eye of each dog. Control group dogs (n = 7) received no medication, whereas those in the treatment groups received a topical anti‐inflammatory medication (difluprednate [DFBA] ophthalmic emulsion 0.05% [n = 7] or betamethasone [BMZ] sodium phosphate ophthalmic solution 0.1% [n = 7]) at 0, 15, 30, and 45 minutes after initial paracentesis in the paracentesed eyes. Secondary aqueous humor (AH) was collected 60 minutes after initial paracentesis. Protein and prostaglandin E2 (PGE2) concentrations in AH were determined using the bicinchoninic acid assay and commercially available immunoassay kit, respectively. All mean values in the three groups were compared using analysis of variance followed by Tukey's post hoc test. Results Aqueous protein and PGE2 concentrations were markedly increased at 60 minutes following paracentesis. Both concentrations in the secondary AH of the DFBA group were significantly lower than those of the control group; however, treatment with BMZ had no significant effects. Conclusions Early postparacentesis treatment with DFBA was more effective than that with BMZ for reducing aqueous protein and PGE2 contents in dogs with paracentesis‐induced BAB breakdown. DFBA may be an appropriate treatment during the early stage of anterior uveitis caused by intraocular surgery in dogs.

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Tumor necrosis factor-alpha concentration in the aqueous humor of healthy and diseased dogs: A preliminary pilot study

Cited by 7 publications

References 16 publications

Definition, Classification, and Pathophysiology of Canine Glaucoma

Definition, Classification, and Pathophysiology of Canine Glaucoma

Genetic and Biochemical Biomarkers in Canine Glaucoma

Comparison of early posttreatment effects of two steroidal anti‐inflammatory ophthalmic drugs on the ocular inflammatory response induced by paracentesis in healthy canine eyes

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