Tumor necrosis factor alpha (TNF-α) blockage reduces acute inflammation and delayed wound healing in oral ulcer of rats

Freitas, Milena Oliveira; Fonseca, Alline Pereira Rodrigues; Aguiar, Maria Thaynara; Dias, Camila Costa; Avelar, Rafael Linard; Sousa, Fabrício Bitú; Alves, Ana Paula Negreiros Nunes; Silva, Paulo Goberlânio de Barros

doi:10.1007/s10787-022-01046-3

Cited by 15 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…31 Also, blocking TNF-α with infliximab, a monoclonal antibody to human TNF-α, has been shown to reduce acute inflammatory response during the development of traumatic oral ulcers, but may delay wound healing in mucosal injuries. 32 Therefore, this cytokine has been shown to play a role in the development of mucositis (phase 2 and 3), as previously demonstrated in the literature, but it may also be important in the healing process (phase 5).…”

Section: Discussionmentioning

confidence: 58%

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

VIANA FILHO,

CASTRO COÊLHO,

QUEIROZ NETO

et al. 2024

Braz. oral res.

View full text Add to dashboard Cite

The aim of this study was to investigate the DNA methylation profile in genes encoding catalase (CAT) and superoxide dismutase (SOD3) enzymes, which are involved in oxidative stress mechanisms, and in genes encoding pro-inflammatory cytokines interleukin-6 (IL6) and tumor necrosis factor-alpha (TNF-α) in the oral mucosa of oncopediatric patients treated with methotrexate (MTX ® ). This was a cross-sectional observational study and the population comprised healthy dental patients (n = 21) and those with hematological malignancies (n = 64) aged between 5 and 19 years. Oral conditions were evaluated using the Oral Assessment Guide and participants were divided into 4 groups: 1-healthy individuals; 2-oncopediatric patients without mucositis; 3-oncopediatric patients with mucositis; 4-oncopediatric patients who had recovered from mucositis. Methylation of DNA from oral mucosal cells was evaluated using the Methylation-Specific PCR technique (MSP). For CAT, the partially methylated profile was the most frequent and for SOD3 and IL6, the hypermethylated profile was the most frequent, with no differences between groups. For TNF-α, the hypomethylated profile was more frequent in the group of patients who had recovered from mucositis. It was concluded that the methylation profiles of CAT, SOD3, and IL6 are common profiles for oral cells of children and adolescents and have no association with oral mucositis or exposure to chemotherapy with MTX ® . Hypomethylation of TNF-α is associated with oral mucosal recovery in oncopediatric patients who developed oral mucositis during chemotherapy.

show abstract

Section: Discussionmentioning

confidence: 58%

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

VIANA FILHO,

CASTRO COÊLHO,

QUEIROZ NETO

et al. 2024

Braz. oral res.

View full text Add to dashboard Cite

show abstract

“…Importantly, the proportion of infiltrating macrophages in the DSeP@PB + RL group was the lowest, being almost 8 times lower than that in the control group. Immunostaining was performed to evaluate the expression of TNF-α, which is implicated in the first stages of inflammatory processes and is released by activated macrophages as a proinflammatory cytokine [ 55 , 56 ] As displayed in Fig. 7 E, the DSeP + RL group had considerably lower TNF-α expression levels than the DSeP group, suggesting that 730 -nm light irradiation could reduce the expression of TNF-α in the DSeP group.…”

Section: Resultsmentioning

confidence: 99%

Selenide-linked polydopamine-reinforced hybrid hydrogels with on-demand degradation and light-triggered nanozyme release for diabetic wound healing

Bei

Pan

et al. 2023

Biomater Res

View full text Add to dashboard Cite

Background Multifunctional hydrogels with controllable degradation and drug release have attracted extensive attention in diabetic wound healing. This study focused on the acceleration of diabetic wound healing with selenide-linked polydopamine-reinforced hybrid hydrogels with on-demand degradation and light-triggered nanozyme release. Methods Herein, selenium-containing hybrid hydrogels, defined as DSeP@PB, were fabricated via the reinforcement of selenol-end capping polyethylene glycol (PEG) hydrogels by polydopamine nanoparticles (PDANPs) and Prussian blue nanozymes in a one-pot approach in the absence of any other chemical additive or organic solvent based on diselenide and selenide bonding-guided crosslinking, making them accessible for large-scale mass production. Results Reinforcement by PDANPs greatly increases the mechanical properties of the hydrogels, realizing excellent injectability and flexible mechanical properties for DSeP@PB. Dynamic diselenide introduction endowed the hydrogels with on-demand degradation under reducing or oxidizing conditions and light-triggered nanozyme release. The bioactivity of Prussian blue nanozymes afforded the hydrogels with efficient antibacterial, ROS-scavenging and immunomodulatory effects, which protected cells from oxidative damage and reduced inflammation. Further animal studies indicated that DSeP@PB under red light irradiation showed the most efficient wound healing activity by stimulating angiogenesis and collagen deposition and inhibiting inflammation. Conclusion The combined merits of DSeP@PB (on-demand degradation, light-triggered release, flexible mechanical robustness, antibacterial, ROS-scavenging and immunomodulatory capacities) enable its high potential as a new hydrogel dressing that can be harnessed for safe and efficient therapeutics for diabetic wound healing.

show abstract

“…During the occurrence and recovery of oral ulcers, a large number of cytokines and chemokines, such as TNF-α, IL-1β, IL-6, and IL-8, were involved in the regulation of inflammatory response and affect the process of oral ulcers. Among these inflammatory factors, TNF-α and IL-6 play a leading role [ 40 , 41 , 42 , 43 ]. Therefore, macrophages (RAW 264.7) were incubated in diluents of 3J or different hydrogels after inflammation induction with Lipopolysaccharide, and the levels of TNF-α and IL-6 were used as evaluation indexes to investigate their anti-inflammatory effects.…”

Section: Resultsmentioning

confidence: 99%

Carboxymethyl Chitosan/Sodium Alginate/Chitosan Quaternary Ammonium Salt Composite Hydrogel Supported 3J for the Treatment of Oral Ulcer

Lin

Chen

Geng

et al. 2023

Gels

View full text Add to dashboard Cite

Oral ulcer is a common inflammatory disease of oral mucosa, causing severe burning pain and great inconvenience to daily life. In this study, compound 3J with anti-inflammatory activity was synthesized beforehand. Following that, an intelligent composite hydrogel supported 3J was designed with sodium alginate, carboxymethyl chitosan, and chitosan quaternary ammonium salt as the skeleton, and its therapeutic effect on the rat oral ulcer model was investigated. The results show that the composite hydrogel has a dense honeycomb structure, which is conducive to drug loading and wound ventilation, and has biodegradability. It has certain antibacterial effects and good anti-inflammatory activity. When loaded with 3J, it reduced levels of TNF-α and IL-6 in inflammatory cells by up to 50.0%. It has excellent swelling and water retention properties, with a swelling rate of up to 765.0% in a pH 8.5 environment. The existence of a large number of quaternary ammonium groups, carboxyl groups, and hydroxyl groups makes it show obvious differences in swelling in different pH environments, which proves that it has double pH sensitivity. It is beneficial to adapt to the highly dynamic changes of the oral environment. Compared with single hydrogel or drug treatment, the drug-loaded hydrogel has a better effect on the treatment of oral ulcers.

show abstract

Tumor necrosis factor alpha (TNF-α) blockage reduces acute inflammation and delayed wound healing in oral ulcer of rats

Cited by 15 publications

References 36 publications

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

Selenide-linked polydopamine-reinforced hybrid hydrogels with on-demand degradation and light-triggered nanozyme release for diabetic wound healing

Carboxymethyl Chitosan/Sodium Alginate/Chitosan Quaternary Ammonium Salt Composite Hydrogel Supported 3J for the Treatment of Oral Ulcer

Contact Info

Product

Resources

About