Tumor-suppressing effects of microRNA-429 in human renal cell carcinoma via the downregulation of Sp1

Wu, Donghai; Niu, Xiaobing; Pan, Huixing; Zhou, Yifei; Zhang, Zichun; Qü, Ping; Zhou, Jian

doi:10.3892/ol.2016.4953

Cited by 27 publications

(22 citation statements)

References 56 publications

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“…miRNAs, a new group of regulatory molecules, have been reported to be abnormally expressed in various types of human cancer and associated with the pathogenesis of tumorigenesis and tumor development (28,29). Therefore, it is of great value to investigate the function of miRNAs in gastric cancer formation and progression, and to examine the underlying molecular mechanisms of their involvement.…”

Section: Discussionmentioning

confidence: 99%

Microrna-136 promotes proliferation and invasion ingastric cancer cells through Pten/Akt/P-Akt signaling pathway

2018

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Abstract. Gastric cancer is the fourth most common cancer and the second most frequent cause of cancer-associated mortality in the world. Previous studies have revealed that expression levels of microRNAs (miRNAs) are associated with the initiation and progression of several types of cancer, including gastric cancer. Previous studies have demonstrated that the abnormal expression of miRNA-136 may serve a function in the progression of several types of human cancer. However, the expression pattern of miR-136, its functions and underlying molecular mechanisms in gastric cancer remain unresolved. In the present study, it was revealed that the expression of miR-136 was aberrantly up regulated in gastric cancer tissues and cell lines. The suppression of miR-136 was able to inhibit proliferation and invasion in gastric cancer cell lines. Furthermore, phosphatase and tensin homolog (PTEN) was identified as a direct target gene of miR-136 in gastric cancer. PTEN was under expressed in gastric cancer tissues compared with non-tumor gastric tissues, and PTEN expression was negatively correlated with miR-136 expression. Furthermore, PTEN overexpression mimics the effects of miR-136 knockdown on gastric cancer cells. Additionally, miR-136 under expression decreased phospho-(p) AKT expression, but did not affect AKT expression in gastric cancer cells. In conclusion, the data of the present study suggest that miR-136 acts as an oncogene in gastric cancer via regulation of the PTEN/AKT/p-AKT signaling pathway and may potentially serve as a novel therapeutic target for the treatment of gastric cancer.

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Section: Discussionmentioning

confidence: 99%

Microrna-136 promotes proliferation and invasion ingastric cancer cells through Pten/Akt/P-Akt signaling pathway

2018

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“…MicroRNA (miRNA) are a class of small, single‐stranded, endogenous non‐coding RNA consisting of 19‐25 nucleotides that interact with the 3′‐untranslated region of the mRNA of target genes to promote mRNA degradation and/or inhibit protein translation . Abnormal expression of miRNA can regulate the biological process by activating or inhibiting oncogenic genes, tumor suppressor genes or target proteins …”

Section: Introductionmentioning

confidence: 99%

“…10,11 Abnormal expression of miRNA can regulate the biological process by activating or inhibiting oncogenic genes, tumor suppressor genes or target proteins. 12,13 Several recently published studies report that lncRNA can have the effect of sponging miRNA, which weakens the impact of miRNA on mRNA according to the theories about RNA regulation by competitive endogenous RNA (ceRNA). 14,15 The primary theory is that RNA could interact with miRNA response elements (MRE).…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of long noncoding RNA associated‐competing endogenous RNA as prognostic biomarkers in clear cell renal carcinoma

et al. 2018

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Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant carcinomas and its molecular mechanisms remain unclear. Long noncoding RNA (lncRNA) could bind sites of miRNA which affect the expression of mRNA according to the competing endogenous (ceRNA) theory. The aim of the present study was to construct a ceRNA network and to identify key lncRNA to predict survival prognosis. We identified differentially expressed mRNA, lncRNA and miRNA between tumor tissues and normal tissues from The Cancer Genome Atlas database. Then, using bioinformatics tools, we explored the connection of 89 lncRNA, 10 miRNA and 22 mRNA, and we constructed the ceRNA network. Furthermore, we analyzed the functions and pathways of 22 differentially expressed mRNA. Then, univariate and multivariate Cox regression analyses of these 89 lncRNA and overall survival were explored. Nine lncRNA were finally screened out in the training group. The patients were divided into high‐risk and low‐risk groups according to the 9 lncRNA and low‐risk scores having better clinical overall survival (P < .01). Furthermore, the receiver operating characteristic curve demonstrates the predicted role of the 9 lncRNA. The 9‐lncRNA signature was successfully proved in the testing group and the entire group. Finally, multivariate Cox regression analysis and stratification analysis further proved that the 9‐lncRNA signature was an independent factor to predict survival. In summary, the present study provides a deeper understanding of the lncRNA‐related ceRNA network in ccRCC and suggests that the 9‐lncRNA signature could serve as an independent biomarker to predict survival in ccRCC patients.

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“…Deregulation of miRNAs has been observed in numerous types of human cancer, such as RB (15), gastric cancer (16), bladder cancer (17) and ovarian cancer (18). miRNAs may serve as either tumour suppressors or oncogenes in different types of human malignancies, and this mainly depends on the functional characteristics of their target genes (19). Lowly expressed miRNAs may normally play tumour-suppressing roles through the regulation of oncogenes (20), whereas upregulated miRNAs may act as oncogenes during tumour initiation and progression by repressing tumour-suppressor genes (21).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑448 inhibits the progression of retinoblastoma by directly targeting ROCK1 and regulating PI3K/AKT signalling pathway

Liu

et al. 2018

Oncol Rep

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Retinoblastoma (RB) is the most common primary intraocular malignancy during infancy and childhood worldwide. Numerous microRNAs (miRNAs) contribute to RB initiation and progression through the regulation of cell proliferation, cycle, apoptosis, migration, invasion and metastasis. Therefore, further investigation concerning the expression, roles and associated mechanisms of RB‑related miRNAs may be beneficial to develop novel strategies for patients with this malignancy. Recently, miRNA‑448 (miR‑448) has been reported to be aberrantly expressed and to play an important role in several types of human cancer. However, the expression patterns and biological roles of miR‑448 in RB have not been studied. The aim of the present study was to detect the expression levels of miR‑448 and investigate its functions in RB and its associated molecular mechanisms. In the present study, miR‑448 was significantly downregulated in RB tissues and cell lines. Upregulation of miR‑448 decreased cell proliferation and invasion and increased apoptosis in RB cells. Additionally, ρ‑associated coiled‑coil containing protein kinase 1 (ROCK1) was validated as a novel direct target gene of miR‑448 in RB. ROCK1 was overexpressed in RB tissues and inversely correlated with miR‑448 expression. Furthermore, ROCK1 silencing induced effects on the proliferation, invasion and apoptosis of RB cells similar to those observed following miR‑448 overexpression. Moreover, restoration of miR‑448 expression markedly reversed the effects of miR‑448 overexpression on RB cells, further supporting the hypothesis that ROCK1 is a direct functional target of miR‑448 in RB. Importantly, miR‑448 targeted ROCK1 to inhibit the activation of the PI3K/AKT signalling pathway in RB. These results demonstrated that miR‑448 may serve as a tumour suppressor in RB by directly targeting ROCK1 and regulating the PI3K/AKT signalling pathway, thereby suggesting that miR‑448 may be an effective therapeutic target for treating this aggressive cancer.

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Tumor-suppressing effects of microRNA-429 in human renal cell carcinoma via the downregulation of Sp1

Cited by 27 publications

References 56 publications

Microrna-136 promotes proliferation and invasion ingastric cancer cells through Pten/Akt/P-Akt signaling pathway

Microrna-136 promotes proliferation and invasion ingastric cancer cells through Pten/Akt/P-Akt signaling pathway

Integrated analysis of long noncoding RNA associated‐competing endogenous RNA as prognostic biomarkers in clear cell renal carcinoma

MicroRNA‑448 inhibits the progression of retinoblastoma by directly targeting ROCK1 and regulating PI3K/AKT signalling pathway

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