Tumour growth modifies intravascular polyamine transport by plasma lipoproteins in the mouse

Catros-Quemener, Véronique; Leray, G; Moulinoux, Jacques-Philippe; Havouis, R; Certaines, J de; Chapman, John

doi:10.1016/s0005-2760(97)00016-7

Cited by 6 publications

(1 citation statement)

References 17 publications

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“…Spermidine in plasma can bind to HDL [ 20 ], which is known to inhibit the formation of oxidized low-density lipoprotein (LDL) [ 21 ]. Polyamines, especially spermine, are also potent antioxidants and anti-inflammatory agents [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

Activity of peroxisomal enzymes, and levels of polyamines in LPA-transgenic mice on two different diets

et al. 2005

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BackgroundIn man, elevated levels of plasma lipoprotein (a)(Lp(a)) is a cardiovascular risk factor, and oxidized phospholipids are believed to play a role as modulators of inflammatory processes such as atherosclerosis. Polyamines are potent antioxidants and anti-inflammatory agents. It was therefore of interest to examine polyamines and their metabolism in LPA transgenic mice.Concentration of the polyamines putrescine, spermidine and spermine as well as the activity of peroxisomal polyamine oxidase and two other peroxisomal enzymes, acyl-CoA oxidase and catalase were measured. The mice were fed either a standard diet or a diet high in fat and cholesterol (HFHC). Some of the mice in each feeding group were in addition given aminoguanidine (AG), a specific inhibitor of diamine oxidase, which catalyses degradation of putrescine, and also inhibits non-enzymatic glycosylation of protein which is implicated in the aetiology of atherosclerosis in diabetic patients. Non-transgenic mice were used as controls.ResultsIntestinal peroxisomal polyamine oxidase activity was significantly higher in LPA transgenic mice than in the non-transgenic mice, while intestinal peroxisomal catalase activity was significantly lower. Hepatic β-oxidation increased in Lp(a) transgenic mice fed the HFHC diet, but not in those on standard diet.Hepatic spermidine concentration was increased in all mice fed the HFHC diet compared to those fed a standard diet, while spermine concentration was decreased. With exception of the group fed only standard diet, transgenic mice showed a lower degree of hepatic steatosis than non-transgenic mice. AG had no significant effect on hepatic steatosis.ConclusionThe present results indicate a connection between peroxisomal enzyme activity and the presence of the human LPA gene in the murine genome. The effect may be a result of changes in oxidative processes in lipid metabolism rather than resulting from a direct effect of the LPA construct on the peroximal gene expression.

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Section: Discussionmentioning

confidence: 99%

Activity of peroxisomal enzymes, and levels of polyamines in LPA-transgenic mice on two different diets

et al. 2005

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Optimization and application of electrophoretic mobility analysis of human red blood cells to study their in vitro stability, interaction with polycations and proteolytic enzymes

Grandfils

Foresto

Riquelme

et al. 2007

J Biomedical Materials Res

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Electrophoretic light scattering method has been considered to determine both the mean and polydispersity of electrophoretic mobility of normal human red blood cells. The final goal of our study was to optimize an in vitro test allowing us to investigate the interaction of xenobiotics, in particular polyelectrolytes, with blood cells. The feasibility of our method has been evaluated based on the reproducibility of our technique to analyze the native electrophoretic mobility of human RBCs, as well as their evolution in the presence of polycationic compounds, i.e. a natural polyamine, spermine, and a synthetic polycation, a polyethylenimine (PEI). Additionally, the follow-up of the human RBCs electrophoretic mobility has been controlled after their incubation with neuraminidase.

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Polyamines as clinical laboratory tools

Gugliucci

2004

Clinica Chimica Acta

120

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Tumour growth modifies intravascular polyamine transport by plasma lipoproteins in the mouse

Cited by 6 publications

References 17 publications

Activity of peroxisomal enzymes, and levels of polyamines in LPA-transgenic mice on two different diets

Activity of peroxisomal enzymes, and levels of polyamines in LPA-transgenic mice on two different diets

Optimization and application of electrophoretic mobility analysis of human red blood cells to study their in vitro stability, interaction with polycations and proteolytic enzymes

Polyamines as clinical laboratory tools

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