Tunable Self-Assembled Peptide Structure: A Novel Approach to Design Dual-Use Biological Agents

Majid, Abdul; Patil-Sen, Yogita; Ahmed, Waqar; Sen, Tapas

doi:10.1016/j.matpr.2017.01.190

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…Husain et al and Morales and co-workers found that decrease in the saturation magnetization value of SPIONs was dependent on the thickness of the silica layer; thicker the silica layer lower is the saturation magnetization (Morales et al 2019, Husain et al 2019. We have previously observed a similar trend for the peptide coated SPIONs and SiSPIONs (Majid et al 2017).…”

Section: Vsm Resultssupporting

confidence: 79%

“…Bare SPIONs (bSPIONs) were prepared by the coprecipitation method using ferrous and ferric chloride, in 1:2 mole ratio in alkaline media and under an inert atmosphere, as previously reported (Majid et al 2017). Briefly, 8.46 g (0.04 mol) FeCl 2 , 4H 2 O and 22.95 g (0.08 mol) FeCl 3 , 6H 2 O were dissolved in 500 ml deionized water previously degassed with nitrogen.…”

Section: Synthesis Of Spionsmentioning

confidence: 99%

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Biocompatible superparamagnetic core-shell nanoparticles for potential use in hyperthermia-enabled drug release and as an enhanced contrast agent

et al. 2020

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Superparamagnetic iron oxide nanoparticles (SPIONs) and core-shell type nanoparticles, consisting of SPIONs coated with mesoporous silica and/or lipid, were synthesised and tested for their potential theranostic applications in drug delivery, magnetic hyperthermia and as a contrast agent. Transmission Electron Microscopy (TEM) confirmed the size of bare and coated SPIONs was in the range of 5–20 nm and 100–200 nm respectively. The superparamagnetic nature of all the prepared nanomaterials as indicated by Vibrating Sample Magnetometry (VSM) and their heating properties under an AC field confirm their potential for hyperthermia applications. Scanning Column Magnetometry (SCM) data showed that extrusion of bare-SPION (b-SPION) dispersions through a 100 nm polycarbonate membrane significantly improved the dispersion stability of the sample. No sedimentation was apparent after 18 h compared to a pre-extrusion estimate of 43% settled at the bottom of the tube over the same time. Lipid coating also enhanced dispersion stability. Transversal relaxation time (T2) measurements for the nanoparticles, using a bench-top relaxometer, displayed a significantly lower value of 46 ms, with a narrow relaxation time distribution, for lipid silica coated SPIONs (Lip-SiSPIONs) as compared to that of 1316 ms for the b-SPIONs. Entrapment efficiency of the anticancer drug, Doxorubicin (DOX) for Lip-SPIONs was observed to be 35% which increased to 58% for Lip-SiSPIONs. Moreover, initial in-vitro cytotoxicity studies against human breast adenocarcinoma, MCF-7 cells showed that % cell viability increased from 57% for bSPIONs to 82% for Lip-SPIONs and to 87% for Lip-SiSPIONs. This suggests that silica and lipid coatings improve the biocompatibility of bSPIONs significantly and enhance the suitability of these particles as drug carriers. Hence, the magnetic nanomaterials prepared in this work have potential theranostic properties as a drug carrier for hyperthermia cancer therapy and also offer enhancement of contrast agent efficacy and a route to a significant increase in dispersion stability.

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Section: Vsm Resultssupporting

confidence: 79%

Section: Synthesis Of Spionsmentioning

confidence: 99%

Biocompatible superparamagnetic core-shell nanoparticles for potential use in hyperthermia-enabled drug release and as an enhanced contrast agent

et al. 2020

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“…In order to study the conformational changes and secondary structures, resulting upon adding different molar concentrations of Zn 2+ to GnRH [6-D-Phe], we used Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR–FTIR). The bands at 1416 cm −1 and 1434–1454 cm −1 indicated an increase in the C-H bending and -C = O stretching vibration, respectively 25 – 27 (Fig. 3a ).…”

Section: Resultsmentioning

confidence: 98%

Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils

Yordanova

Vanderlinden

Stoll

et al. 2018

Sci Rep

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A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures and fibrils upon a pH shift in the presence of different concentrations of Zn2+ in vitro. Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR–FTIR) revealed the existence of higher order assembly of Zn2+: GnRH [6-D-Phe]. Nuclear Magnetic Resonance spectroscopy (NMR) indicated a weak interaction between Zn2+ and GnRH [6-D-Phe]. Atomic Force Microscopy (AFM) showed the existence of GnRH [6-D-Phe] oligomers and fibrils. Molecular Dynamic (MD) simulation of the 10:1 Zn2+: GnRH [6-D-Phe] explored the interaction and dimerization processes. In contrast to already existing short peptide fibrils, GnRH [6-D-Phe] nanostructures and fibrils form in a Tris-buffered pH environment in a controlled manner through a temperature reduction and a pH shift. The lyophilized Zn2+: GnRH [6-D-Phe] assembly was tested as a platform for the sustained delivery of GnRH [6-D-Phe] and incorporated into two different oil vehicle matrices. The in vitro release was slow and continuous over 14 days and not influenced by the oil matrix.

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“…It had been established that removal of the N-terminus charge via addition of a Boc group could not stop the formation of H-Phe-Phe-OH like structures (Levin et al, 2014). Boc-Phe-Phe-OH in different solvent conditions was observed to display tuneable structures of two different morphologies: tubular or spherical under different solvent conditions and the presence/absence of superparamagnetic iron oxide (SPIONs) core and studied for dual use (drug delivery and heat generation) (Majid et al, 2017). Also, molecular dynamics simulations had suggested that the N-terminus stabilizes the initial stage aggregates during the self-assembly formation while in the final tube structure, it does not rely on the charged termini regions.…”

Section: Dipeptides For Biologics Deliverymentioning

confidence: 99%

Ultrashort Peptide Self-Assembly: Front-Runners to Transport Drug and Gene Cargos

Gupta

Singh

Sharma

et al. 2020

Front. Bioeng. Biotechnol.

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The translational therapies to promote interaction between cell and signal come with stringent eligibility criteria. The chemically defined, hierarchically organized, and simpler yet blessed with robust intermolecular association, the peptides, are privileged to make the cutoff for sensing the cell-signal for biologics delivery and tissue engineering. The signature service and insoluble network formation of the peptide self-assemblies as hydrogels have drawn a spell of research activity among the scientists all around the globe in the past decades. The therapeutic peptide market players are anticipating promising growth opportunities due to the ample technological advancements in this field. The presence of the other organic moieties, enzyme substrates and well-established protecting groups like Fmoc and Boc etc., bring the best of both worlds. Since the large sequences of peptides severely limit the purification and their isolation, this article reviews the account of last 5 years' efforts on novel approaches for formulation and development of single molecule amino acids, ultra-short peptide self-assemblies (di-and tri-peptides only) and their derivatives as drug/gene carriers and tissue-engineering systems.

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Tunable Self-Assembled Peptide Structure: A Novel Approach to Design Dual-Use Biological Agents

Cited by 8 publications

References 21 publications

Biocompatible superparamagnetic core-shell nanoparticles for potential use in hyperthermia-enabled drug release and as an enhanced contrast agent

Biocompatible superparamagnetic core-shell nanoparticles for potential use in hyperthermia-enabled drug release and as an enhanced contrast agent

Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils

Ultrashort Peptide Self-Assembly: Front-Runners to Transport Drug and Gene Cargos

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