Tunable Structures and Properties of Electrospun Regenerated Silk Fibroin Mats Annealed in Water Vapor at Different Times and Temperatures

Huang, Xiangyu; Fan, Suna; Altayp, Alhadi Ibrahim Mohammed; Zhang, Yaopeng; Shao, Huili; Hu, Xuechao; Xie, Minkai; Xu, Yuhong

doi:10.1155/2014/682563

Cited by 21 publications

(19 citation statements)

References 33 publications

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“…The X-ray diffraction profiles for the films produced from the mild solvent systems given in Figure 15 b show diffraction peaks around 8.1, 16.1, and 20.6° with corresponding crystalline spacings of 10.9, 5.5, and 4.3 Å, respectively. These peaks all correspond to β-sheet crystallinity [ 34 ]. An additional peak of 23.9° indicative of β-sheets with a spacing of 3.7 Å was observed only for LiBr produced films.…”

Section: Resultsmentioning

confidence: 99%

A Comparative Study on the Dissolution of Argema mimosae Silk Fibroin and Fabrication of Films and Nanofibers

et al. 2021

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Limited studies have been done on silk fibroins of wild silkworm species owing to their relative insolubility in many solvents. In this study, the solubility of Argema mimosae wild silk fibroin in different salts (LiBr, LiCl, Ca(NO3)2, and CaCl2) dissolved in formic acid under varying temperatures was investigated. The dissolution conditions under which the solubility was optimum were optimized using a central composite design approach. The optimum range for solvation of the fibroin were visualized using contour plots. The influence of temperature and salt concentration were found to significantly influence the solvation of the fibroin. Following the successful dissolution of the fibroin, the regenerated silk fibroin solutions were cast to obtain water insoluble films which were used in investigating optimum electrospinning conditions. Average nanofiber diameters in the 110–141 nm range were obtained under optimum electrospinning conditions. The silk forms were characterized using the FTIR, TGA, XRD, and SEM to understand their properties. The investigations revealed that formic acid—salt solvents were effective in the solvation of the wild silk fibroin. Some of the dissolution conditions induced mild effects on the silk fibroin while others were harsh. Furthermore, processing to nanofibers resulted in the degradation of the β-sheets producing nanofibers rich in α-helices. However, post-treatment using methanol and water vapor were effective in restoring β-sheet crystallinity.

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Section: Resultsmentioning

confidence: 99%

A Comparative Study on the Dissolution of Argema mimosae Silk Fibroin and Fabrication of Films and Nanofibers

et al. 2021

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“…The characteristic peak at 1632 cm −1 attributed to an amide I β-sheet and the peak at 1260 cm −1 attributed to amide III are due to random coil. So, when the nanoparticles of silk fibroin were formed both the silk I crystal structure and the silk II crystal structure of silk fibroin were observed [24,25]. The assignments of the FTIR bands of silk fibroin nanoparticles are presented in detail in Table 2.…”

Section: Resultsmentioning

confidence: 99%

Silk Fibroin Nanoparticles for Drug Delivery: Effect of Bovine Serum Albumin and Magnetic Nanoparticles Addition on Drug Encapsulation and Release

et al. 2018

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Silk fibroin nanoparticles were prepared in the present study based on phase separation between silk fibroin and polyvinyl alcohol. The drug encapsulation efficiency of the prepared nanoparticles was examined at a range of concentrations from 10 ppm to 500 ppm for pramipexole, curcumin, and propranolol hydrochloride. Silk fibroin nanoparticles encapsulated with propranolol presented the highest drug release profile. In order to improve the drug encapsulation efficiency and drug release performance, a modification of silk fibroin nanoparticles with bovine serum albumin and magnetic nanoparticles was tried. The modification was found to improve the drug encapsulation and release of the modified nanoparticles. Bovine-serum-modified nanoparticles presented the best improvement.

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“…While in the hydrogel, the decreased β-sheet structure can be attributed to the effect of pepsin digestion on proteins as previously demonstrated by Yang et al . 48 , in the ES-patch, changes can be attributed to the electrospinning process, which was previously shown to cause major conformational changes in the secondary structure of different proteins 31,49,50 .…”

Section: Discussionmentioning

confidence: 96%

3D Structure and Processing Methods Direct the Biological Attributes of ECM-Based Cardiac Scaffolds

Efraim

Schoen

Zahran

et al. 2019

Sci Rep

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High hopes are held for cardiac regenerative therapy, driving a vast research effort towards the development of various cardiac scaffolds using diverse technologies and materials. Nevertheless, the role of factors such as fabrication process and structure in determining scaffold’s characteristics is yet to be discovered. In the present study, the effects of 3D structure and processing method on cardiac scaffolds are addressed using three distinct scaffolds made through different production technologies from the same biomaterial: decellularized porcine cardiac extracellular matrix (pcECM). pcECM patch, injectable pcECM hydrogel, and electrospun pcECM scaffolds were all proven as viable prospective therapies for MI, thus generally preserving pcECM beneficial properties. Yet, as we demonstrate, minor differences in scaffolds composition and micro-morphology as well as substantial differences in their mechanical properties, which arise from their production process, highly affect the interactions of the scaffold with both proliferating cells and functional cells. Hence, the rates of cell attachment, survival, and proliferation significantly vary between the different scaffolds. Moreover, major differences in cell morphology and alignment as well as in matrix remodeling are obtained. Overall, the effects revealed herein can guide a more rational scaffold design for the improved cellular or acellular treatment of different cardiac disease scenarios.

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Tunable Structures and Properties of Electrospun Regenerated Silk Fibroin Mats Annealed in Water Vapor at Different Times and Temperatures

Cited by 21 publications

References 33 publications

A Comparative Study on the Dissolution of Argema mimosae Silk Fibroin and Fabrication of Films and Nanofibers

A Comparative Study on the Dissolution of Argema mimosae Silk Fibroin and Fabrication of Films and Nanofibers

Silk Fibroin Nanoparticles for Drug Delivery: Effect of Bovine Serum Albumin and Magnetic Nanoparticles Addition on Drug Encapsulation and Release

3D Structure and Processing Methods Direct the Biological Attributes of ECM-Based Cardiac Scaffolds

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