2020
DOI: 10.3390/ijms21176181
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Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs

Abstract: Fat tissue represents an important source of adipose-derived stem cells (ADSCs), which can differentiate towards several phenotypes under certain stimuli. Definite molecules as vitamin D are able to influence stem cell fate, acting on the expression of specific genes. In addition, miRNAs are important modulating factors in obesity and numerous diseases. We previously identified specific conditioned media able to commit stem cells towards defined cellular phenotypes. In the present paper, we aimed at evaluating… Show more

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Cited by 13 publications
(11 citation statements)
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“…The same Figure shows that ADSCs cultured in MD alone exhibited a typical mature adipocyte morphology (Figure 1). Our results are further inferred by previous observation by our group [33], in which we demonstrated the ability of these two molecules to oppose adipogenic differentiation, albeit in the presence of a specific adipogenic conditioned medium. The number of mature adipocytes was calculated using ImageJ.…”
Section: Metformin and Vitamin D Inhibit Adsc Adipogenic Differentiationsupporting
confidence: 91%
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“…The same Figure shows that ADSCs cultured in MD alone exhibited a typical mature adipocyte morphology (Figure 1). Our results are further inferred by previous observation by our group [33], in which we demonstrated the ability of these two molecules to oppose adipogenic differentiation, albeit in the presence of a specific adipogenic conditioned medium. The number of mature adipocytes was calculated using ImageJ.…”
Section: Metformin and Vitamin D Inhibit Adsc Adipogenic Differentiationsupporting
confidence: 91%
“…In addition, metformin reduced the levels of TNF-α in obese mice, down-regulating NF-kB translocation into macrophages [40]. We recently described the effect of metformin, alone or in combination with vitamin D, in controlling ADSC adipogenic differentiation, acting on vitamin D metabolism through epigenetic modification and miRNAs [33]. Within this context, in the present study, we aimed to evaluate the ability of the two molecules to modulate inflammation and autophagy, both closely related to obesity and unproper activation of adipocytes.…”
Section: Discussionmentioning
confidence: 99%
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“…Nevertheless, some issues are still open. In particular, the clear evidence that stem cell behavior and fate are influenced by the cellular microenvironment [ 5 , 6 , 7 , 10 , 37 ] raises more than a cautionary note on the use of a stem cell source in patients who underwent mastectomy as a part of a breast cancer treatment.…”
Section: Discussionmentioning
confidence: 99%
“…Human adipose-derived stem cells (hADSCs) are able to differentiate into different cell lineages upon stimulation using a conditioned medium, as demonstrated in several studies [ 5 , 6 , 7 ].…”
Section: Introductionmentioning
confidence: 99%