Turkey herpesvirus with an insertion in the UL3-4 region displays an appropriate balance between growth activity and antibody-eliciting capacity and is suitable for the establishment of a recombinant vaccine

Andoh, Kiyohiko; Yamazaki, Kaoru; Honda, Yoko; Honda, Takashi

doi:10.1007/s00705-016-3181-4

Cited by 10 publications

(6 citation statements)

References 39 publications

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“…Effectively, the OmpH expression cassettes were integrated into the UL45/UL46 region and consistently maintained within the recombinant viruses for up to 15 passages. These findings align with prior research that has similarly established UL45/UL46 as suitable sites for foreign gene integration [ 20 , 22 , 36 ]. Moreover, the possible application of multivalent rHVT vaccine in different species of poultry for the control of fowl cholera was proved using antibody response and protection efficacy against P. multocida in ducks.…”

Section: Discussionsupporting

confidence: 90%

Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing Pasteurella multocida OmpH Protein for Fowl Cholera Prevention in Ducks

Apinda,

Yao,

Zhang

et al. 2023

Vaccines

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Fowl cholera is caused by the bacterium Pasteurella multocida, a highly transmissible avian ailment with significant global implications, leading to substantial economic repercussions. The control of fowl cholera outbreaks primarily relies on vaccination using traditional vaccines that are still in use today despite their many limitations. In this research, we describe the development of a genetically engineered herpesvirus of turkeys (HVT) that carries the OmpH gene from P. multocida integrated into UL 45/46 intergenic region using CRISPR/Cas9-NHEJ and Cre-Lox system editing. The integration and expression of the foreign cassettes were confirmed using polymerase chain reaction (PCR), indirect immunofluorescence assays, and Western blot assays. The novel recombinant virus (rHVT-OmpH) demonstrated stable integration of the OmpH gene even after 15 consecutive in vitro passages, along with similar in vitro growth kinetics as the parent HVT virus. The protective efficacy of the rHVT-OmpH vaccine was evaluated in vaccinated ducks by examining the levels of P. multocida OmpH-specific antibodies in serum samples using ELISA. Groups of ducks that received the rHVT-OmpH vaccine or the rOmpH protein with Montanide™ (SEPPIC, Paris, France) adjuvant exhibited high levels of antibodies, in contrast to the negative control groups that received the parental HVT or PBS. The recombinant rHVT-OmpH vaccine also provided complete protection against exposure to virulent P. multocida X-73 seven days post-vaccination. This outcome not only demonstrates that the HVT vector possesses many characteristics of an ideal recombinant viral vaccine vector for protecting non-chicken hosts, such as ducks, but also represents significant research progress in identifying a modern, effective vaccine candidate for combatting ancient infectious diseases.

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Section: Discussionsupporting

confidence: 90%

Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing Pasteurella multocida OmpH Protein for Fowl Cholera Prevention in Ducks

Apinda,

Yao,

Zhang

et al. 2023

Vaccines

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“…To exclude any potential adverse effects of gene modification on viral replication, the UL45/UL46 intergenic junction, previously proven to be suitable for insertion of foreign genes in several studies [5] , [7] , [34] , was selected as the target locus for the generation of recombinant HVT in our study ( Fig. 1 ).…”

Section: Resultsmentioning

confidence: 99%

A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system

Tang¹,

Zhang

Pedrera

et al. 2018

Vaccine

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“…Due to the expression of the same antigen at different sites possibly leading to different efficacies of recombinant HVT vaccines [ 15 , 27 , 36 ], two recombinant HVT viruses, rHVT-005/006-F and rHVT-US2-F, were constructed. Both expressed the modified F protein of NDV genotype VII at either the US2 site or HVT-005/006.…”

Section: Discussionmentioning

confidence: 99%

“…The selection of protective antigens and determination of insertion sites are crucial for the efficacy of recombinant vaccines [ 6 , 15 , 27 ]. A stable new insertion site, HVT-005/006 [ 28 ], has been previously identified, but its potential to express the protective antigen of NDV deserves further evaluation.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Protection against Virulent Newcastle Disease Virus (NDV) in Chickens Immunized with a Single Dose of Recombinant Turkey Herpesvirus Expressing NDV F Protein

Shi,

Yang,

Xiao

et al. 2024

Vaccines

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Newcastle disease (ND) is a significant infectious disease in poultry, causing substantial economic losses in developing countries. To control ND, chickens must be vaccinated multiple times a year. In order to develop an improved vaccine that provides long-term protection, the F gene from genotype VII NDV was inserted into the herpesvirus of turkey (HVT) vaccine virus using CRISPR/Cas9-mediated NHEJ repair and Cre/LoxP technology. The immunogenicity and protective efficacy of the resulting recombinant vaccines were evaluated through antibody assays and virus challenge experiments. Two recombinant vaccines, rHVT-005/006-F and rHVT-US2-F, were generated, both exhibiting growth rates comparable with those of HVT in vitro and consistently expressing the F protein. One-day-old specific pathogen-free (SPF) chickens immunized with 2000 PFU/bird of either rHVT-005/006-F or rHVT-US2-F developed robust humoral immunity and were completely protected against challenge with the NDV F48E8 strain at 4 weeks post-vaccination (wpv). Furthermore, a single dose of these vaccines provided sustained protection for at least 52 wpv. Our study identifies rHVT-005/006-F and rHVT-US2-F as promising ND vaccine candidates, offering long-term protection with a single administration. Moreover, HVT-005/006 demonstrates promise for accommodating additional foreign genes, facilitating the construction of multiplex vaccines.

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Turkey herpesvirus with an insertion in the UL3-4 region displays an appropriate balance between growth activity and antibody-eliciting capacity and is suitable for the establishment of a recombinant vaccine

Cited by 10 publications

References 39 publications

Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing Pasteurella multocida OmpH Protein for Fowl Cholera Prevention in Ducks

Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing Pasteurella multocida OmpH Protein for Fowl Cholera Prevention in Ducks

A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system

Long-Term Protection against Virulent Newcastle Disease Virus (NDV) in Chickens Immunized with a Single Dose of Recombinant Turkey Herpesvirus Expressing NDV F Protein

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