Turn-on and label-free fluorescence detection of lead ions based on target-induced G-quadruplex formation

Xu, Lijun; Hong, Shanni; Wang, Jine; Zhang, Yuanyuan; Wang, Hongyan; Zhang, Jianye; Pei, Renjun

doi:10.1039/c5cc01590a

Cited by 41 publications

(20 citation statements)

References 47 publications

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“…Pei et al [170] used G4 aptamer-based fluorometric biosensors for cation detection. They proposed a sensing strategy for Pb 2+ based on target-induced G4 formation and found a G-rich sequence (AGRO100) that works as a Pb 2+ aptamer and forms a G4 conformation induced by Pb 2+ .…”

Section: G-quadruplex-containing Nucleic Acid Aptamersmentioning

confidence: 99%

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

Umar

Chan

et al. 2019

Molecules

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Guanine (G)-quadruplexes (G4s) are unique nucleic acid structures that are formed by stacked G-tetrads in G-rich DNA or RNA sequences. G4s have been reported to play significant roles in various cellular events in both macro- and micro-organisms. The identification and characterization of G4s can help to understand their different biological roles and potential applications in diagnosis and therapy. In addition to biophysical and biochemical methods to interrogate G4 formation, G4 fluorescent turn-on ligands can be used to target and visualize G4 formation both in vitro and in cells. Here, we review several representative classes of G4 fluorescent turn-on ligands in terms of their interaction mechanism and application perspectives. Interestingly, G4 structures are commonly identified in DNA and RNA aptamers against targets that include proteins and small molecules, which can be utilized as G4 tools for diverse applications. We therefore also summarize the recent development of G4-containing aptamers and highlight their applications in biosensing, bioimaging, and therapy. Moreover, we discuss the current challenges and future perspectives of G4 fluorescent turn-on ligands and G4-containing aptamers.

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Section: G-quadruplex-containing Nucleic Acid Aptamersmentioning

confidence: 99%

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

Umar

Chan

et al. 2019

Molecules

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“…[51] In this study,i ti ss hown that only Pb 2 + can induce G-quadruplex formation of Spinach; this consequently leads to af luorescencee nhancement at l = 264 nm owing to the binding of Spinachw ith DFHBI. [52] 4.2. [52] 4.2.…”

Section: Metal-mediated G-quadruplex Assaysmentioning

confidence: 99%

“…In addition, the assay also exhibits good selectivity for Pb 2 + over 12 other cat-ions, including K + and Na + ,w ithin the detection range of 100 nm to 5 mm.T his platform also shows performance for Pb 2 + detection in tap water.I nar elated strategy,P ei and coworkers have developed as witch-on Pb 2 + sensorb ased on the selectiver ecognition of NMM to the Pb 2 + -induced G-quadruplex structure. [52]…”

Section: Metal-mediated G-quadruplex Assaysmentioning

confidence: 99%

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The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

Dong

et al. 2017

Chemistry An Asian Journal

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G-Quadruplexes can be induced to form guanine-rich DNA sequences by certain small molecules or metal ions. In concert with an appropriate signal transducer, such as a fluorescent dye or a phosphorescent metal complex, the ligand-recognition event can be transduced into a luminescent response. This focus review aims to highlight recent examples of aptamer-based and metal-mediated G-quadruplex assays for the detection of small molecules and toxic substances in the last three years. We discuss the mechanisms and features of the different assays and present an outlook and a perspective for the future of this field.

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“…Guanine-rich (G-rich) nucleic acid sequences, existing in the region of gene promoter and chromosome telomeres, can fold into a unique G-quadruplex structure in the presence of metal ion, such as potassium ion (K + ) 22 23 . Based on these characteristics, many G-quadruplex-based sensors have been developed for the detection of metal ions 24 25 26 , DNA 27 28 , small molecules 29 30 and protein biomarkers 31 . Such K + -mediated G-quadruplex-based sensing platforms are convenient, low-cost and relatively quick.…”

mentioning

confidence: 99%

Application of iridium(III) complex in label-free and non-enzymatic electrochemical detection of hydrogen peroxide based on a novel “on-off-on” switch platform

Miao

Yang

Leung

et al. 2016

Sci Rep

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We herein report a label-free and non-enzymatic electrochemical sensor for the highly sensitive detection of hydrogen peroxide (H2O2) based on a novel “on-off-on” switch system. In our design, MB was used as an electron mediator to accelerate the electron transfer while AuNPs was used to amplify the electrochemical signal due to its excellent biocompatibility and good conductivity. The “switch-off” state was achieved by introducing the guanine-rich capture probe (CP) and an iridium complex onto the electrode surface to form a hydrophobic layer, which then hinders electron transfer. Upon addition of H2O2, fenton reaction occurs and produces OH• in the presence of Fe2+. The OH• cleaves the CP into DNA fragments, thus resulting in the release of CP and iridium complex from the sensing interface, recovering the electrochemical signal to generate a “switch-on” state. Based on this novel switch system, a detection limit as low as 3.2 pM can be achieved for H2O2 detection. Moreover, satisfactory results were obtained by using this method for the detection of H2O2 in sterilized milk. To the best of our knowledge, this is the first G-quadruplex-based electrochemical sensor using an iridium(III) complex.

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Turn-on and label-free fluorescence detection of lead ions based on target-induced G-quadruplex formation

Abstract: Using a guanine-rich sequence (AGRO100) and N-methyl mesoporphyrin IX (NMM), a turn-on and label-free fluorescent Pb(2+) sensor with high sensitivity and low background fluorescence was presented as a representative of five turn-on sensing systems.

Cited by 41 publications

References 47 publications

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

Application of iridium(III) complex in label-free and non-enzymatic electrochemical detection of hydrogen peroxide based on a novel “on-off-on” switch platform

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