Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione

Chen, Xiaoxiao; Li, Pu; Wu, Gaojun; Wang, Zhe; Huang, Chaobiao

doi:10.1039/d1ra08107a

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…This strategy can encode the thiol metabolite information into DNA sequence only mixing the functional magnetic beads and the sample solution. Although the range to quantify the GSH in this method was almost similar to other GSH measurement assays (Table S2), [13] this strategy can collect released DNA from beads with mRNA by precipitation technique. Therefore, this feature prompted us to establish the seamless DNA encoding and reverse transcription (Figure S6).…”

Section: Resultsmentioning

confidence: 83%

Encoding Thiol Metabolite Information into a DNA Sequence by Disulfide Exchange Reaction on Oligodeoxynucleotides for Parallel Analysis of the Metabolite and mRNA

et al. 2023

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We designed a methodology to encode the thiol metabolite information into a DNA sequence. Thiol metabolites, such as glutathione (GSH) significantly contributes to regulating redox balance in the cells. We constructed a molecular system to quantify GSH by releasing DNA from magnetic beads upon the disulfide exchange reaction. We designed the molecular system as follows: for the conversion of thiol metabolite information into DNA sequence, we used a disulfide exchange reaction between the disulfide (reaction site) tethered oligonucleotide and thiol group of target metabolites. GSH and mRNA in the cell extract were successfully quantified from the samples obtained by DNA encoding and reverse transcription. The present protocol allowed parallel analysis of thiol metabolite and mRNA.

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Section: Resultsmentioning

confidence: 83%