Turn-on trivalent cation selective chemodosimetric probe to image native cellular iron pools

Abstract

A new turn-on cell permeable chemodosimetric probe has been developed and its application in the selective detection of trivalent cations (Fe(3+)/Cr(3+)/Al(3+)) at a sub-nanomolar level has been demonstrated. The selectivity of over a broad spectrum of mono- and divalent metal ions was established using fluorescence spectroscopy. Moreover, the changes in the absorption spectra of in the presence of trivalent cations enabled the most bio-relevant metal ion Fe(3+) over Cr(3+)/Al(3+) to be distinguished. The probe was found to be successful in the fluorescence imaging of native cellular iron pools. The fluorescence imaging of the native iron pools of banana pith further supported the high sensitivity of towards Fe(3+) present in living systems. To the best of our knowledge, this is the first example of a turn-on chemodosimetric probe to image native cellular Fe(3+) pools.

Selective chromo-fluorogenic detection of trivalent cations in aqueous environments using a dehydration reaction

“…The LOD were in the mM range and were similar to those calculated for other recently published probes for trivalent cations. [9][10][11][12][13][14][15][16][17][18][19][20][21] Moreover, we also found that water-acetonitrile 95 : 5 v/v solutions of 3 showed, when excited at 300 nm, an intense emission band at 397 nm. From all the cations tested, only the addition of Cr 3+ , Fe 3+ , Al 3+ , Ga 3+ , In 3+ and As 3+ induced a marked emission quenching (see Fig.…”

“…7 For these reasons, the development of optical chemosensors able to discriminate trivalent from divalent and monovalent cations 8 is especially appealing. In particular, there are relatively few probes able to detect simultaneously several trivalent cations [9][10][11][12][13][14][15][16][17][18][19] and we have found in the literature only one probe that recognizes Al 3+ , Fe 3+ , Cr 3+ , As 3+ , In 3+ and Ga 3+ at the same time. 20 Moreover, most probes for trivalent cations display sensing features in organic solvents and it is rare to find probes for these cations in pure water.…”

Recent Advances on Iron(III) Selective Fluorescent Probes with Possible Applications in Bioimaging

“…Similar to the other chemodosimeters, probe 76 has been successfully applied to detect intracellular Fe 3+ ions in live RAW264.7 cells by fluorescence microscopy. Adopting the hydrolytic cleavage of imine linkage, the multi-analytes selective chemodosimeter 77 has been developed for the detection of trivalent metal ions (Fe 3+ , Cr 3+ , and Al 3+ ) in THF-H 2 O (8:2) medium [86]. The strong Lewis acidity of trivalent cations (Fe 3+ /Al 3+ /Cr 3+ ) selectively breaks the imine linkage, resulting in significant fluorescence enhancement at 430 nm (λ exc = 330 nm).…”

A TBET based BODIPY-rhodamine dyad for the ratiometric detection of trivalent metal ions and its application in live cell imaging

“…Even though several fluorescent probes are available for the detection of divalent or trivalent ions, probes useful for the collective detection of trivalent metal ions are very few in number. In the last couple of years, few reports based on 4 the chemosensors for trivalent metal ion detection are published in various international journals [29][30][31][32][33][34]. Most of these fluorescent probes operate based on metal ion induced fluorescence 'turn-on' or 'turn-off' mechanisms.…”