Twelve novel polymorphic microsatellites in a marine fish species, yellow croaker Larimichthys polyactis

Abstract

We isolated 12 polymorphic microsatellites from an important marine food fish Larimichthys polyactis and characterized them in 32 unrelated individuals. Among the 12 microsatellites, four were tetranucleotide repeats and eight were dinucleotide repeats. The allele number ranged from five to 25 with an average of 15.4/locus; average expected heterozygosity was 0.81, ranging from 0.57 to 0.95, whereas the observed heterozygosity ranged from 0.34 to 1.00 (average: 0.78). Nine of the 12 markers conformed to Hardy–Weinberg equilibrium and showed no sign of linkage. These microsatellites will be useful for population genetic studies and selective breeding programs of this species.

Short Communication Development and testing of 13 polymorphic microsatellite markers in Larimichthys polyactis (Sciaenidae) using 5' anchored PCR

“…The H O and H E per locus ranged from 0.3214 to 1.000 and from 0.3097 to 0.8567, with an average of 0.6816 and 0.6724, respectively. Compared with the heterozygosity of L. polyactis, the heterozygosity in our study was higher than that of the Dalian population (Wang et al, 2009), but lower than that of another population (Li et al, 2006). Compared with the heterozygosity of other species, such as Epinephelus awoara (0.598; Dong et al, 2007) and Verasper moseri (0.60; , the heterozygosity in our study was higher.…”

“…Polymorphic microsatellite markers have been reported for this important species, L. polyactis (Li et al, 2006;Wang et al, 2009); however, they are very limited for the study of genetic diversity and conservation for this species. Thus, it is necessary to isolate more polymorphic microsatellite markers for this fish species.…”

Genetic structure ofLarimichthys polyactis(Pisces: Sciaenidae) in the Yellow and East China Seas inferred from microsatellite and mitochondrial DNA analyses

“…In this study, four primer pairs for small yellow croaker microsatellite markers, previously developed by Li et al (2006), were used. The forward primer of each set was labelled with one of the following fluorescent dyes: 6FAM, NED or HEX.…”

“…The forward primer of each set was labelled with one of the following fluorescent dyes: 6FAM, NED or HEX. The process of PCR amplification, using a PTC-200 thermal cycler (MJ Research, Reno, NV, USA), was conducted as follows: a first denaturation step at 948C for 2 min; 35 cycles of 30 s at 948C, 30 s at the annealing temperature listed by Li et al (2006) and 30 s at 728C; and a final extension for 5 min at 728C. To screen for microsatellite polymorphisms, the size of fluorescence-labelled allele fragments was measured on an ABI PRISM 3130XL automated sequencer, followed by analysis with GeneMapper version 3.7 (Applied Biosystems, Foster, CA, USA).…”

“…The microsatellite loci are often considered more powerful molecular markers in detecting population heterogeneity than mtDNA RFLP or allozyme datasets (Estoup et al 1998;Ross et al 1999), because the former are highly polymorphic and biparentally inherited. Although microsatellite markers for L. polyactis were developed previously by Li et al (2006), no population genetic studies using these markers in field samples have been undertaken. In addition, it is necessary to study the population structure of L. polyactis using a lessvariable mtDNA such as the gene encoding cytochrome c oxidase subunit I (COI), because the noncoding control region may not reflect true relationship among populations.…”