Twelve polymorphic microsatellites in Oriental river prawn, <i>Macrobrachium nipponense</i>

Feng, Jianbin; Li, J. L.

doi:10.1111/j.1755-0998.2008.02129.x

Cited by 11 publications

(9 citation statements)

References 6 publications

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“…Because different broodstocks or seedstocks might differ in growth rate, or disease resistance, among other characteristics (Klinbunga et al, 2001), the identification of wild shrimp stocks is also important to provide an available source of wild genetic diversity in domestication and selective breeding programs (Brooker et al, 2000;Klinbunga et al, 2001). Currently, relative studies on M. nipponense have mainly focused on the development of molecular markers (Feng and Li, 2008;Zhao et al, 2010;Qiao et al, 2011;Song and Kim, 2011) and genetic diversity of the wild populations from the south rivers, mainly in the Yangtze River Basin and Qiantang River (Fu et al, 2010;Feng et al, 2011;Ge et al, 2011;Ma et al, 2011;Su et al, 2011). Previous studies on the genetic diversity of wild M. nipponense were mainly focused on the geographic populations of Yangtze River, Qiantang River, Taihu Lake, Poyang Lake, and Hongze Lake.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker

Qiao¹,

Lv²,

Jiang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. To assess the genetic status of this species, the genetic diversity of wild Macrobrachium nipponense from seven geographic locations in the Yellow River basin were investigated using 20 polymorphic microsatellite DNA loci. The genetic diversity between populations was indicated by the mean number of alleles per locus and mean observed heterozygosity (H) and the expected H, which was arranged from 2 to 10, from 0.4705 to 0.5731, and from 0.5174 to 0.6146, respectively. Hardy-Weinberg equilibrium analysis indicated that a deficiency of heterozygotes existed in all seven populations. Both the F ST and AMOVA analyses showed that there is significant difference on population differentiation among populations. The UPGMA clustering tree demonstrated that their close relationship is consistent with their geographic proximity. The Genetic diversity analysis of Oriental River Prawn data suggest that this Yellow River population has a wide genetic base that is suitable for breeding.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker

Qiao¹,

Lv²,

Jiang³

et al. 2013

Genet. Mol. Res.

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“…Colony PCR was conducted on a Eppendorf PCR machine to determine the insert length. Colony polymerase chain reaction was: cloning of the bacteria samples were denatured for 5 min at 94°C, then 30 cycles for 30 s at 94°C, 1 min at 60°C and 1 min at 72°C, followed by a final extension of 10 min at 72°C (Feng and Li 2008). Clones with inserts from 250 to 1000 bp were sequenced on an ABI PRISM 3730 DNA sequencer.…”

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confidence: 99%

Twenty-four novel polymorphic microsatellite markers from oriental river prawn (Macrobrachium nipponense)

Feng

et al. 2010

Conservation Genet Resour

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The oriental river prawn (Macrobrachium nipponense) is an important freshwater prawn species in China. We reported the isolation and characterization of 24 novel polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA) 15 and (CT) 15 probes. The variability of these microsatellites were tested on 60 individuals collected from Hongze Lake (China). The average allele number was 15.2 per locus, ranging from 9 to 25. The observed heterozygosity was from 0.1333 to 0.9667 and the expected heterozygosity was from 0.8391 to 0.9570. 9 of the 24 microsatellites did not conform to Hardy-Weinberg equilibrium, whereas all the microsatellite loci have shown a high degree of polymorphism information content (PIC [ 0.5). These microsatellites can be used to study economic traits of QTL position, population genetic diversity and the construction of the genetic mapping for Macrobrachium nipponense in the future.

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“…Deficiency of heterozygotes in marine invertebrates is not uncommon (Maggioni and Rogers 2001;Puebla et al 2003;Shearer and Coffroth 2004;Damodaran et al 2008;Feng and Li 2008;Costantini et al 2007). The reason for the observed departure from HWE seen in this species may be a result of unrecognised polymorphisms in one or both of the primer sequences leading to the presence of an apparent ''null'' allele.…”

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confidence: 96%

Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)

Groth

Lim

Lestang

et al. 2009

Conservation Genet Resour

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Nine microsatellite loci were identified in the western rock lobster (Panulirus cygnus) using two different methods. The first method involved the screening of a small, fragment, partial genomic library with a radioactive (CA) 6 probe. The second method, was based upon an enrichment method and used biotinylated, tetranucleotide microsatellite oligonucleotide capture probes. The nine loci described are all very polymorphic, with 11 to 34 alleles observed for each locus and heterozygosities ranging from 0.58 to 0.86. These microsatellite loci will be useful in analysing both the population structure and the mating systems used by this species and will add important information for the management of the wild stocks of this economically important species.Keywords Panulirus cygnus Á Microsatellite Á SSR The western rock lobster (Panulirus cygnus) is Western Australia's most important single species fishery with annual harvests over the last decade ranging between approximately 9 and 14.5 thousand tonnes per annum. The elucidation and understanding of population and mating structures for this species will allow the information obtained to be incorporated into an improved management strategy for the brood stock. In order to understand the population structure and mating systems of the western rock lobster, polymorphic, microsatellite genetic markers have been isolated and characterised. Two strategies were used to develop these microsatellites, the first consisting of the construction of a Hae III small fragment library which was screened with a (CA)n probe and the second library was made using an enrichment technique followed by screening with tetranucleotide microsatellite motifs. Nine microsatellite loci have been fully characterised and these are described in this report.A DNA genomic library was made using Qiagen Tissue Kit (Qiagen) to isolate DNA extracted from tail muscle tissue. The DNA was digested with HaeIII restriction endonuclease (Promega, CA, USA) followed by excision of the 100-500 bp region after agarose (1%) gel electrophoresis. Fragments were ligated into Sma I (Promega) digested, dephosphorylased pUC18 plasmid vector and transformed into competent cells (DH5a Invitrogen). Colonies were transferred to Hybond N? membranes and screened with radiolabelled (CA)10 probe. Thirty clones were identified after the first round of screening. Following successive screening rounds to further isolate the clones, twenty of these were sequenced. Plasmid DNA was isolated from the positive clones and sequenced using cyclesequencing combined with IRD 800 labelled primers on a Li-Cor gene sequencer. Primers were designed using Mac Vector (Eastman-Kodak, USA) or PRIMER 3 software (Rozen and Skaletsky 2000). Many of the loci identified contained insufficient flanking DNA sequences or produced microsatellites containing few repeat units. Three loci (WRL 1, WRL 2 and WRL 3) identified by using this method were deemed to be useful.A second library enriched for tetranucleotide microsatellites was prepared using genomic DNA. Br...

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Twelve polymorphic microsatellites in Oriental river prawn, Macrobrachium nipponense

Cited by 11 publications

References 6 publications

Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker

Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker

Twenty-four novel polymorphic microsatellite markers from oriental river prawn (Macrobrachium nipponense)

Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)

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