Twin-column CaptureSMB: A novel cyclic process for protein A affinity chromatography

Angarita, Monica; Müller‐Späth, Thomas; Baur, Daniel; Lievrouw, Roel; Lissens, Geert; Morbidelli, Massimo

doi:10.1016/j.chroma.2015.02.046

Cited by 145 publications

(89 citation statements)

References 22 publications

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“…However, when scaled up to commercial size, the cost difference became negligible, as more Amsphere resin was required per harvest. 23 Such differences in the Amsphere resin performance between the two studies could be due to the variations in the experimental conditions and that DBC values were measured at 10% breakthrough in the study reported by McCaw et al, 22 which can contribute to product losses. 24 Overall, the authors showed that a multicolumn chromatography system such as CaptureSMB could save up to 30% of production cost compared to standard batch chromatography.…”

Section: Affinity Chromatographymentioning

confidence: 99%

“…This system utilizes affinity chromatography instead of ion-exchange in MCSGP. 23 In this system, Amsphere resin showed a more stable DBC at flow rates higher than 300 cm h −1 compared to MabSelect SuRe. It was also calculated that the annual cost of Amsphere resin used in small-scale proof-of-concept experiments using the CaptureSMB system was approximately 25% less than that of MabSelect SuRe resin.…”

Section: Affinity Chromatographymentioning

confidence: 99%

“…23,43,44 The smaller columns used in these systems reduce affinity resin and buffer consumption by 40%-50%, while sequential loading of the columns with the flow-through from the previous column ensures maximum product capture. 23,43 Also, having all the columns in operation at any given point of time, albeit at different stages of the process, significantly reduces production times.…”

Section: Advances In Chromatography-based Purification Technologiesmentioning

confidence: 99%

“…23 The countercurrent movement of the liquid and the stationary phase, called simultaneous moving bed (SMB), is the basic principle in various continuous and semicontinuous countercurrent sequential loading systems that can utilize up to 12 columns simultaneously. 23,43,44 The smaller columns used in these systems reduce affinity resin and buffer consumption by 40%-50%, while sequential loading of the columns with the flow-through from the previous column ensures maximum product capture. 23,43 Also, having all the columns in operation at any given point of time, albeit at different stages of the process, significantly reduces production times.…”

Section: Advances In Chromatography-based Purification Technologiesmentioning

confidence: 99%

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Technology advancements in antibody purification

Murphy¹,

Devine²,

O’Kennedy³

2016

ANTI

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Abstract:The separation of antibodies from complex mixtures can be achieved using chromatographic or non-chromatographic techniques. The purification of antibodies using chromatography involves the separation of antibodies or antibody-derived molecules present in complex mixtures by passing them through a solid phase (eg, silica resin or beads, monolithic columns, or cellulose membranes) and allowing the antibodies to bind or pass through depending on whether "bind-and-elute" or "flow-through" chromatographic methods are employed. Chromatographic methodologies can incorporate different separation techniques, such as affinity-tag binding, ion-exchange, size-exclusion chromatography, or immunoaffinity chromatography. However, in a concerted effort to become less reliant on chromatography-based separations owing to the high cost of large-scale production, non-chromatographic-based techniques such as precipitation, flocculation, crystallization, filtration, and aqueous two-phase partitioning are now becoming more popular. This review details current chromatographic and non-chromatographic methodo logies used for the purification of antibodies and expands on technological advancements and practical uses that have recently been reported.

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Section: Affinity Chromatographymentioning

confidence: 99%

Section: Affinity Chromatographymentioning

confidence: 99%

Section: Advances In Chromatography-based Purification Technologiesmentioning

confidence: 99%

Section: Advances In Chromatography-based Purification Technologiesmentioning

confidence: 99%

See 2 more Smart Citations

Technology advancements in antibody purification

Murphy¹,

Devine²,

O’Kennedy³

2016

ANTI

View full text Add to dashboard Cite

show abstract

“…The system is based on periodical feed delivery and product withdrawal. Recently, twin-column CaptureSMB has been adapted for the affinity capture of a monoclonal antibody [19].…”

Section: Introductionmentioning

confidence: 99%