Two acidic polysaccharides from the flowers of Chrysanthemum morifolium

Abstract

Two new acidic polysaccharides, F4 and F5, were isolated from the flowers of Chrysanthemum morifolium. Monosaccharide analysis indicated that F4 contained arabinose, galactose and galacturonic acid units in a molar ratio of 1.0:2.3:6.8 and F5 contained arabinose, rhamnose galactose and galacturonic acid units in a molar ratio of 1.0:3.2:1.0:4.3. The results of methylation analysis, partial acid hydrolysis and NMR spectral analysis indicated that F4 had a homogalacturonan main chain with arabinogalactan side chain linked to 3 position of (1 --> 3,4)-linked galacturonan and F5 had a rhamnogalacturonan main chain with arabinogalactan side chain linked to 3 position of (1 --> 3,4)-linked galacturonan or 4 position of (1 --> 2,4)-linked rhamnose. Biological tests revealed that F4 and F5 could simulate the mitogen induced T and B lymphocyte proliferation in vitro.

Neurite Outgrowth in PC12 Cells Stimulated by Components fromDendranthema×grandiflorumcv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK

“…The pharmacological effects of Chrysanthemum morifolium , including Mottenohoka, are various and include sleep induction [1], protection of brain cells against ischemia [2], toxicity to cancer cells [3], suppression of intercellular adhesion molecule-1 and E-selectin expression [4], protection of neuroblastoma SH-SY5Y cells [5], cardiac protection against ischemia [6], anti-HIV effect [7, 8], and antimutagenicity [9]. Components of Chrysanthemum morifolium that may be associated with these pharmacological effects are luteolin and apigenin [10, 11], acidic polysaccharide [3, 12], dicaffeoylquinic acids [13], chlorogenic acid, 3,5- O -dicaffeoylquinic acid, 3′,4′,5-trihydroxyflavanone 7- O -glucuronide [14], apigenin 7- O - β - d -(4′-caffeoyl)glucuronide and apigenin 7- O - β - d -glucuronide [7], acacetin, apigenin, luteolin and quercetin [9], and acacetin 7- O - β - d -galactopyranoside [8]. …”

Structural characterization, antioxidant activity, and antiglycation activity of polysaccharides from different chrysanthemum teas

“…As shown in Table 2, the molecular weights of fraction 1 and fraction 2 in JHP-1, JHP-2, JHP-3, JHP-4, and JHP-5 ranged from 4.29 Â 10 5 Da to 5.88 Â 10 5 Da, and from 4.11 Â 10 4 Da to 5.24 Â 10 4 Da, respectively, which are similar with previous studies. 10,42,43 The highest molecular weight of fraction 1 was measured in JHP-5 among all tested JHPs, and the lowest molecular weight was observed in JHP-1. The low molecular weights of natural polysaccharides may contribute to their relatively high antioxidant effects in vitro.…”

“…Results showed that GalA, Ara, and Gal were the dominant monosaccharides in JHPs extracted from different chrysanthemum teas, which further conrmed that JHPs were pectic-polysaccharides. 33,43 Furthermore, rhamnogalacturonan I (RG I), homogalacturonan (HG), and arabinogalactan (AG II) might exist in JHPs from different chrysanthemum teas based on the constituent monosaccharides determined in JHPs. 10,33,42,43…”

“…33,43 Furthermore, rhamnogalacturonan I (RG I), homogalacturonan (HG), and arabinogalactan (AG II) might exist in JHPs from different chrysanthemum teas based on the constituent monosaccharides determined in JHPs. 10,33,42,43…”

Structural elucidation of a polysaccharide from Chrysanthemum morifolium flowers with anti-angiogenic activity

“…They are reported to possess the ability of clearing heat and detoxification, and in clinic traditionally used for headache, influenza, hepatic and eye diseases. But according to previous literatures [7,8], researches on their polysaccharides are very limited, particular with the respect to structural characterization. Xyloglucan is the component of plant cell wall and a kind of hemicellulose.…”