Two B-cell epitope vaccines based on uPA effectively inhibit fertility in male mice

Ding, Xiaofang; Li, Huimin; Li, Yuanyuan; Huang, Donghui; Xiong, Chengliang

doi:10.1016/j.vaccine.2018.03.071

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…In a recent review it was described that plasmin was involved in many immunological processes by directly interacting with various cell types including monocytes, macrophages, dendritic cells and vascular wall cells (endothelial cells, smooth muscle cells) as well as soluble factors of the immune system and components of the ECM [13]. Furthermore, uPA could become a new target for immunocontraception in developing male contraceptives [14]. Thus, the regulation mechanisms of uPA in Sertoli cells require further studies.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat Sertoli cells

Liu

Zhao

Xiong

et al. 2021

Folia Histochem Cytobiol.

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Section: Introductionmentioning

confidence: 99%

Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat Sertoli cells

Liu

Zhao

Xiong

et al. 2021

Folia Histochem Cytobiol.

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“…In a similar study, it was observed that immunization with complete uPA protein might have serious side effects and induce immune tolerance at higher doses while using B-cell epitopes reduce adverse effects and enhance the immune specificity [51,52]. The purpose of this study was to design a new vaccine, consisting of three spermatic proteins (IZUMO1, SACA3, and PH-20), which can induce B cells and results in contraception by the immune response.…”

Section: Introductionmentioning

confidence: 99%

Designing a Construct of Chimeric Multi-Epitopes Protein for Contraceptive Vaccine in Mice: An Immunoinformatics and In Silico Study

et al. 2020

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Background: Contraceptive vaccines (CVs) can be used as a valuable and alternative method for the prevention of gestation in humans and animals. These vaccines can have several targets, such as superficial sperm proteins. Vaccines based on sperm antigens are quite efficacious to create a contraceptive effect. However, multi-epitope vaccines are more effective in stimulating the immune system and producing more antibodies to reduce the infertility rate. Materials and Methods: This study aimed to design and evaluate a chimeric fusion protein containing IZUMO, SACA3, and PH-20 epitopes. IZUMO1, SACA3, and PH-20 were assessed, and appropriate regions were selected using various bioinformatics tools, including IEDB, I-TASSER, ProtParam, Asa-View, and Chimera software. Protein epitopes were selected based on various characters, including specificity, solvent accessibility, their weight and length, antigenic intensity, and topology. Epitopes with high antigenic potential were selected and joined together by linkers. The designed fusion protein was simulated using Molecular Dynamic, GROMACS 5, and Chimera 1.14 software. Results: The results demonstrated that all antigenic plots and availability of epitopes in the new construct remained constant. The spermatic antigens were combined using rigid linkers as a new construct and showed a stable formation with proper solvent accessibility validated by ProSA-web and PROCHECK. Also, comparing the new structure with its original one did not show any structural change. Conclusion: Based on bioinformatics results, the fusion protein that consists of three spermatic antigens has productive potential to stimulate the immune system and capable of producing more antibodies in circulation and reliable infertility.

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Evaluation of multi-epitope recombinant protein as a candidate for a contraceptive vaccine

Mortazavi

Fard

Karkhane

et al. 2021

Journal of Reproductive Immunology

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Two B-cell epitope vaccines based on uPA effectively inhibit fertility in male mice

Cited by 6 publications

References 29 publications

Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat Sertoli cells

Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat Sertoli cells

Designing a Construct of Chimeric Multi-Epitopes Protein for Contraceptive Vaccine in Mice: An Immunoinformatics and In Silico Study

Evaluation of multi-epitope recombinant protein as a candidate for a contraceptive vaccine

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