Two‐coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms

Abstract

Complex coacervation of alginate-chitosan and alginate-gelatin were used to develop two-coat systems for the encapsulation of Spathoglottis plicata seeds and protocorms (top-shaped structures formed after seed germination of orchids). Both the seeds and the protocorms could withstand the encapsulation treatments with high viability. About 54% of seeds and 40% of large protocorms (1.6-2.0 mm) were able to tolerate a 6-h desiccation treatment. However, viability of the small protocorms (0.7-0.9 mm) was greatly reduced if they were desiccated before encapsulation. Encapsulation after desiccation significantly increased the percentage viability of seeds and protocorms. Treatment with abscisic acid (ABA, 10 −5 M) before desiccation and encapsulation resulted in high percentage viability in seeds and large protocorms whereas the small protocorms were found to be less tolerant to the treatments.

Chitosan Amphiphilic Derivatives. Chemistry and Applications

“…It was found that turbidity of aqueous solutions of grafted chitosan with added SDS exhibits a sudden rise and a subsequent sharp fall due to the aggregation, deaggregation and precipitation of complexes depending on the SDS/cation molar ratio. Chitosanpolyvinyl pyrrolidone hydrogels with pH sensitivity have also been prepared by using 60 Co gamma-irradiation [157].…”