Two DevBCA‐like ABC transporters are involved in the multidrug resistance of the cyanobacterium <i>Anabaena</i> sp. PCC 7120

Symbiosis between cyanobacteria and plants is considered pivotal for biological nitrogen deposition in terrestrial ecosystems. Despite extensive knowledge of the ecology of plant-cyanobacterium symbioses, little is known about the molecular mechanisms involved in recognition between partners. Here, we conducted a quantitative sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS) pipeline to analyse protein changes in Oryza sativa and Nostoc punctiforme during early events of symbiosis. We found differentially expressed proteins in both organisms linked to several biological functions, including signal transduction, adhesion, defence-related proteins, and cell wall modification. In Nostoc punctiforme we found increased expression in 62 proteins that have been previously described in other Nostoc-plant symbioses, reinforcing the robustness of our study. Our findings reveal new proteins activated in the early stages of the Nostoc-Oryza symbiosis that might be important for the recognition between the plant and the host. Oryza mutants in genes in the common symbiosis signalling pathway (CSSP) show a reduced colonization efficiency providing first insights on the involvement of the CSSP for the accommodation of N. punctiforme inside the plant cells. This information may have long-term implications for a greater understanding of the symbiotic interaction between Nostoc and land plants.

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“…In Nostoc sp. PCC 7120, this exporter is essential for cyanobacterial envelope formation ( Shvarev et al. 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk

Álvarez

Brenes‐Álvarez

Molina-Heredia

et al. 2022

Plant and Cell Physiology

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“…While some of these have partially overlapping functions and are involved in heterocyst-specific roles, at least some of these DevBCA paralogues such as the tripartite pumps formed of the gene products of alr4280/alr4281/alr4282 and alr3647/ alr3648/alr3649 that code for the components of putative MacB-like ABC exporters have been shown to be essential for resistance to a range of different drugs and efflux of ethidium bromide, while having no demonstrable role in heterocyst development. 564 In addition, the YbbAP is a poorly characterized member of the MacB-family in E. coli, 419 where ybbA encodes the predicted NBD subunit and ybbP encodes the predicted permease subunit of this putative exporter complex. Although association with heavy metal export has been suggested, 418 this member of the family remains poorly understood.…”

Section: Macb/ftsx Transporter Familymentioning

confidence: 99%

“…The HgdD OMF protein is central to secretion and efflux in Anabaena , as it has been shown to pair with at least seven different DevBCA paralogues, with varied functions. While some of these have partially overlapping functions and are involved in heterocyst-specific roles, at least some of these DevBCA paralogues such as the tripartite pumps formed of the gene products of alr4280/alr4281/alr4282 and alr3647/alr3648/alr3649 that code for the components of putative MacB-like ABC exporters have been shown to be essential for resistance to a range of different drugs and efflux of ethidium bromide, while having no demonstrable role in heterocyst development …”

Section: Classes Of Transporter Proteinsmentioning

confidence: 99%

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria

et al. 2021

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Tripartite efflux pumps and the related type 1 secretion systems (T1SSs) in Gram-negative organisms are diverse in function, energization, and structural organization. They form continuous conduits spanning both the inner and the outer membrane and are composed of three principal components—the energized inner membrane transporters (belonging to ABC, RND, and MFS families), the outer membrane factor channel-like proteins, and linking the two, the periplasmic adaptor proteins (PAPs), also known as the membrane fusion proteins (MFPs). In this review we summarize the recent advances in understanding of structural biology, function, and regulation of these systems, highlighting the previously undescribed role of PAPs in providing a common architectural scaffold across diverse families of transporters. Despite being built from a limited number of basic structural domains, these complexes present a staggering variety of architectures. While key insights have been derived from the RND transporter systems, a closer inspection of the operation and structural organization of different tripartite systems reveals unexpected analogies between them, including those formed around MFS- and ATP-driven transporters, suggesting that they operate around basic common principles. Based on that we are proposing a new integrated model of PAP-mediated communication within the conformational cycling of tripartite systems, which could be expanded to other types of assemblies.

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“…Interestingly, YbbA belongs to MacB-like ABC transporters but does not form tripartite pumps and seems to be involved in lipoprotein export. MacB-like ABC transporters have been shown to be essential for drug resistance in cyanobacterium . The trimethylated residue of YbbA is within the MacB-like ATP-binding domain.…”

Section: Resultsmentioning

confidence: 99%

“…MacB-like ABC transporters have been shown to be essential for drug resistance in cyanobacterium. 91 The trimethylated residue of YbbA is within the MacB-like ATPbinding domain.…”

Section: K-trimethylated Proteins Were Distributed Across All Biologi...mentioning

confidence: 99%

Lysine Trimethylation in Planktonic and Pellicle Modes of Growth in Acinetobacter baumannii

et al. 2023

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Over the past 30 years, Acinetobacter baumannii has been described as an important nosocomial pathogen due to frequent ventilator-associated infections. Many biological processes of A. baumannii remain elusive, such as the formation of an air–liquid biofilm (pellicle). Several studies demonstrated the importance of post-translational modifications (PTMs) in A. baumannii physiology. Here, we investigated K-trimethylation in A. baumannii ATCC 17978 in planktonic and pellicle modes using proteomic analysis. To identify the most high-confidence K-trimethylated peptides, we compared different sample preparation methods (i.e., strong cation exchange, antibody-capture) and processing software (i.e., different database search engines). We identified, for the first time, 84 K-trimethylated proteins, many of which are involved in DNA and protein synthesis (HupB, RplK), transporters (Ata, AdeB), or lipid metabolism processes (FadB, FadD). In comparison with previous studies, several identical lysine residues were observed acetylated or trimethylated, indicating the presence of proteoforms and potential PTM cross-talks. This is the first large-scale proteomic study of trimethylation in A. baumannii and will be an important resource for the scientific community (availability in Pride repository under accession PXD035239).

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Two DevBCA‐like ABC transporters are involved in the multidrug resistance of the cyanobacterium Anabaena sp. PCC 7120

Cited by 9 publications

References 44 publications

Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk

Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria

Lysine Trimethylation in Planktonic and Pellicle Modes of Growth in Acinetobacter baumannii

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