Two-Dimensional Gel Electrophoresis (2-DE)

Baudin, Bruno

doi:10.5772/38312

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Two-dimensional gel electrophoresis (2D-GE) is one of the best known 2D separation methods for proteins and peptides, in which analytes are first separated based on the difference in isocratic points, and second by molecular size [4,5]. Two-dimensional HPLC (2D-HPLC) is also frequently utilized for the separation of biological, medical, and pharmaceutical samples [6].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer

Ito¹,

Matsugi²,

Kitagawa³

et al. 2016

Chromatography

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Fabrication of a monolithic polymer layer, as the stationary phase, equipped with two electrodes was studied for simultaneous two-dimensional separation, combining high performance liquid chromatography and orthogonal electrophoresis. The monolithic polymer layer was prepared between a pair of glass plates by a two-step UV-initiated photo-polymerization. Prior to preparation of the monolithic polymer layer for separation, high-density monolith bars were prepared along the electrodes to suppress the penetration of bubbles formed at the electrodes into the separation monolith. Using the monolithic polymer layer, 2D separation of colored-dyes was successfully achieved by combining gradient HPLC and orthogonal electrophoresis. The 2D separation of proteins was also demonstrated.

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Section: Introductionmentioning

confidence: 99%

Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer

Ito¹,

Matsugi²,

Kitagawa³

et al. 2016

Chromatography

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Two-Dimensional Electrophoresis and Identification by Mass Spectrometry

Cuesta

Alvarez-Llamas

Barderas³

2017

Methods in Molecular Biology

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Two-dimensional electrophoresis (2-DE) is a classical separation technique for complex protein mixtures based on a first-separation step by isoelectric point (pI) followed by SDS-PAGE. Due to its high resolving power (including protein isoforms) and the capability to display post-translational modifications, 2-DE has been the technique of choice in many studies focused in characterizing allergens. In this chapter, we describe a standard protocol which enables efficient protein separation and subsequent protein identification by tandem mass spectrometry (MALDI-MS/MS).

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A Proteomic Approach to Abiotic and Biotic Stress in Barley: A Review

Rodríguez-Vázquez

Carrieri

2023

Plant Mol Biol Rep

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Two-Dimensional Gel Electrophoresis (2-DE)

Cited by 3 publications

References 36 publications

Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer

Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer

Two-Dimensional Electrophoresis and Identification by Mass Spectrometry

A Proteomic Approach to Abiotic and Biotic Stress in Barley: A Review

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