Two-Dimensional High-Performance Liquid Chromatography as a Powerful Tool for Bioanalysis: The Paradigm of Antibiotics

Papatheocharidou, Christina; Samanidou, Victoria

doi:10.3390/molecules28135056

Cited by 9 publications

(4 citation statements)

References 48 publications

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“…Two-dimensional liquid chromatography is an important method for the separation and analysis of complex samples, and it holds an important position in the field of chromatography technology (Papatheocharidou & Samanidou, 2023). We have previously established an HPLC-UV method for determining the concentration of meropenem in synovial fluid.…”

Section: Discussionmentioning

confidence: 99%

Two‐dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection

Jia‐Wei,

Jing,

et al. 2023

Biomedical Chromatography

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Periprosthetic joint infection (PJI) is a catastrophic complication following joint replacement surgery. One potential treatment approach for PJI could be the combination of one‐stage revision and intra‐articular infusion of antibiotics. Meropenem is one of the commonly used intra‐articular antibiotics in our institution. Determining the concentration of meropenem in the joint cavity could be crucial for optimizing its local application, effectively eradicating biofilm infection, and improving PJI treatment outcomes. In this study, we developed a simple, precise, and accurate method of two‐dimensional liquid chromatography (2D‐LC) for determining the concentration of meropenem in human synovial fluid. The method was then validated based on the guidelines of the Food and Drug Administration and the Chinese Pharmacopoeia. Meropenem showed good linearity in the range of 0.31–25.01 μg/mL (r ≥ .999). Selectivity, intra‐day and inter‐day precision and accuracy, extraction recovery, and stability validation results were all within the acceptance range. This method has been successfully applied to the determination of synovial fluid samples from PJI patients, providing a useful detection method for meropenem therapeutic drug monitoring (TDM) in PJI patients.

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Section: Discussionmentioning

confidence: 99%

Two‐dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection

Jia‐Wei,

Jing,

et al. 2023

Biomedical Chromatography

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“…Currently, a gamut of technologies exists to extract and isolate proteins from intricate mixtures to further analyze them. One-and two-dimensional electrophoresis, or highperformance liquid chromatography (HPLC), are the most common uncoupling methods, but mass spectrometry (MS) is now seen as the benchmark [95][96][97]. Advances in quantitative MS through the application of stable isotope labeling and scanning techniques, as well as multiple reaction monitoring (MRM), have greatly enhanced the specificity and sensitivity of MS-based assays [98].…”

Section: Potential Use Of Point-of-care Platforms For the Screening O...mentioning

confidence: 99%

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Guzman,

Guzman

2024

IJTM

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Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage of formation, these disorders can be effectively treated, leading to successful outcomes; curative therapies can halt illnesses from advancing, thus improving the quality of life and long-term survival of the patient. Still, cutting-edge upgrades in precision technologies are necessary for early, reliable, affordable, and rapid disease detection, but also vital for the well-being of people and the future of global public health. The emerging role and utility of non-invasive and repeatable diagnostic test approaches for the detection of health conditions have been exemplified by liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator or signature (e.g., proteins) to provide valuable information on an individual’s wellness and/or disease. Among the bodily secretions used for non-invasive diagnostics is sputum, a complex viscous gel-like biopolymeric network that has gained growing recognition as a rich source of biomarkers of airway infections and pulmonary diseases, and serves as a determinant to reveal other illnesses. As per the World Health Organization, the burden of respiratory conditions is exacerbated by factors ranging from considerable subjection to air pollution and occupational contaminants to tobacco smoking and second-hand smoke, in addition to poor socio-economic status. Due to the likely increase in these determinants, respiratory tract ailments are on the rise, affecting the health of many individuals, in addition to putting stress on healthcare facilities and services worldwide. The aim of this study was to perform a narrative review of sputum constituents with an emphasis on proteins and glycoproteins assessed as possible biomarkers of lung and other organ diseases. A search was conducted using mucus, sputum proteomics, sputum biomarkers, and point-of-care testing as keywords employing Google, PubMed (MEDLINE), and Web of Science, selecting the most referenced and related papers of the last decade. We, therefore, highlight the need to use expectorated or induced sputum specimens as a routine sample source for testing valuable protein biomarkers to diagnose these chronic disorders, predict inflammation and disease progression, as well as monitor the effectiveness of treatments. Further, we discuss the urgent need for fast and reliable point-of-care methods to detect and quantify crucial protein biomarkers in sputum specimens, and the limitations faced when dealing with their complex matrices.

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“…Currently, there is a substantial accumulation of research on the identification and content measurements of components in herbs, including high-performance liquid chromatography, high-performance liquid chromatography-MS, and gas chromatography-MS, etc. ( Arrizabalaga-Larrañaga et al, 2021 ; Papatheocharidou and Samanidou, 2023 ), but there is still a lack of databases for comprehensive aggregation and compilation of quantitative research data on components in herbs.…”

Section: Herb Databasesmentioning

confidence: 99%

Multi-level advances in databases related to systems pharmacology in traditional Chinese medicine: a 60-year review

Fan,

Jin,

et al. 2023

Front. Pharmacol.

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The therapeutic effects of traditional Chinese medicine (TCM) involve intricate interactions among multiple components and targets. Currently, computational approaches play a pivotal role in simulating various pharmacological processes of TCM. The application of network analysis in TCM research has provided an effective means to explain the pharmacological mechanisms underlying the actions of herbs or formulas through the lens of biological network analysis. Along with the advances of network analysis, computational science has coalesced around the core chain of TCM research: formula-herb-component-target-phenotype-ZHENG, facilitating the accumulation and organization of the extensive TCM-related data and the establishment of relevant databases. Nonetheless, recent years have witnessed a tendency toward homogeneity in the development and application of these databases. Advancements in computational technologies, including deep learning and foundation model, have propelled the exploration and modeling of intricate systems into a new phase, potentially heralding a new era. This review aims to delves into the progress made in databases related to six key entities: formula, herb, component, target, phenotype, and ZHENG. Systematically discussions on the commonalities and disparities among various database types were presented. In addition, the review raised the issue of research bottleneck in TCM computational pharmacology and envisions the forthcoming directions of computational research within the realm of TCM.

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Two-Dimensional High-Performance Liquid Chromatography as a Powerful Tool for Bioanalysis: The Paradigm of Antibiotics

Cited by 9 publications

References 48 publications

Two‐dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection

Two‐dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

Multi-level advances in databases related to systems pharmacology in traditional Chinese medicine: a 60-year review

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