Two‐dimensional Hydrophilic Interaction/Reversed‐phase Liquid Chromatography for the Preparative Separation of Polar and Non‐polar Taxanes

Abstract

Combination of hydrophilic and reversed-phase chromatography in two-dimensional liquid chromatography (2D-LC) systems is a useful method for the separation of compounds with a large polarity range such as taxanes.

Taxus ingredients in the red arils of Taxus baccata L. determined by HPLC‐MS/MS

“…Today, the analysis of Taxus ingredients is performed mostly by high performance liquid chromatography (HPLC) using photodiode array (PDA) detection [12][13][14][15][16][17][18][19][20][21][22][23][24][25] and mass spectrometry (MS, MS/MS, laser desorption/ionisation) detection. 10,21,[25][26][27][28][29][30][31] Besides nuclear magnetic resonance (NMR) 32,33 and fluorescence 34 detection was described.…”

Bioassay-Guided Separation and Identification of a New Anti-Lung Cancer Compound from Cordyceps militaris by Means of Off-Line Two-Dimensional Preparative Chromatography, Real-Time Cell Analysis, and X-ray Single-Crystal Diffraction

“…Two-dimensional RP-RP LC is a practical separation method and has been used for isolation of compounds from pharmaceutical samples [22]. Two-dimensional HILIC coupled with RP chromatography has been proved to be very useful for separation of the components of Taxus baccata L. [23]. Although these preparative 2D HPLC systems enable reasonable separation of compounds with a wide range of polarity and can, to some extent, solve the problem of peak capacity and separation selectivity, there are still specific deficiencies in comprehensive characterization of weakly polar TCM extracts, because of limitations of the modes of separation and the complexity of the samples.…”

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

“…Taxane is one of the best known natural compounds that have been used in the pharmaceutical industry. A reasonable chromatographic method based on multidimensional heart‐cutting HILIC and RPLC for simultaneous separation of polar and nonpolar taxanes was introduced . In the 1st D, taxanes were separated from polar compounds using a semipreparative CN column, and in the 2nd D the fraction containing taxanes was transferred to a C18 column after valve switching.…”