Two‐dimensional molecular profiling of mantle cell lymphoma

Antonucci, Francesca; Chilosi, Marco; Parolini, Claudia; Hamdan, Mahmoud; Astner, Hubert; Righetti, Pier Giorgio

doi:10.1002/elps.200305457

Cited by 37 publications

(25 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies on hematologic malignancies using proteomics approaches have been reported, 4,5 but application of proteomics technology to HL is still limited. Fujii et al compared HL cell line proteome to anaplastic large cell lymphoma (ALCL) and non-Hodgkin lymphoma (NHL) using a 2-dimensional difference gel electrophoresis approach of total cell lysates.…”

Section: Introductionmentioning

confidence: 99%

Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes

Yue¹,

Visser²,

Roelofsen³

et al. 2008

Blood

117

View full text Add to dashboard Cite

IntroductionHodgkin lymphoma (HL) is characterized by a minority of neoplastic cells, the Hodgkin and Reed-Sternberg cells (HRS cells), and an extensive inflammatory background. Many studies have documented that HL is associated with disturbed cytokine production. 1 Cytokine and chemokine production may not only promote growth of HRS cells and help to evade immune surveillance, but also cause the characteristic histology and the clinical symptoms of HL. 2 Cross-talk between HRS cells and surrounding lymphocytes has been studied for many years, and this interaction is regarded to be important for the pathogenesis of HL.The application of proteomics techniques has been proven to be a powerful tool for the identification of new biomarkers involved in pathogenesis of many diseases (reviewed by Hanash 3 ). Some studies on hematologic malignancies using proteomics approaches have been reported, 4,5 but application of proteomics technology to HL is still limited. Fujii et al compared HL cell line proteome to anaplastic large cell lymphoma (ALCL) and non-Hodgkin lymphoma (NHL) using a 2-dimensional difference gel electrophoresis approach of total cell lysates. 6,7 HL expressed higher levels of pyridoxine-5Ј-phosphate oxidase, vinculin, dihydropyrimidinase-related protein-2 and NADHubiquinone oxidoreductase compared with ALCL. In comparison to NHL cell lines, HL cell lines showed higher expression of pyridoxine-5Ј-phosphate oxidase, ␥-enolase, vinculin, vimentin, galectin-1, annexin A5, and protein kinase C substrate. 7 Carvalho et al identified changes in the spectrum using quadrupole-time-of-flight hybrid mass spectrometer in serum of HL patients compared with normal controls, but no further protein identification was performed. 8 Overall, only very limited proteomics data are available for HL.To gain more insight into the proteins secreted by HRS cells that might be involved in the cross-talk with infiltrating lymphocytes or might serve as tumor biomarkers, the secretome of HL cell lines was determined in cell culture supernatant. Protein identification was performed by nanoscale reversed-phase liquid chromatography tandem mass spectrometry (LC-MS/MS) after 1D-SDS-PAGE fractionation. Proteins related to immune response were validated in cell culture supernatant and patient plasma by enzyme-linked immunosorbent assay (ELISA) and in tumor tissues by immunohistochemistry. Methods Cell lines and cultureThe HL cell lines L1236 and KMH2 were established from HL patients with mixed cellularity subtype, L428 from nodular sclerosis subtype and DEV from nodular lymphocyte predominant HL (NLPHL) subtype. They were cultured in RPMI 1640 medium (Lonza Walkersville, Walkersville, MD) supplemented with fetal calf serum (Lonza Walkersville). For analysis of secreted proteins, to avoid the masking effects of high-abundance serum proteins in the culture medium, the cells were washed 3 times with RPMI 1640 medium to deplete all serum proteins, and cultured in RPMI 1640 medium without serum for 24 hours. Under these conditions, cell via...

show abstract

Section: Introductionmentioning

confidence: 99%

Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes

Yue¹,

Visser²,

Roelofsen³

et al. 2008

Blood

117

View full text Add to dashboard Cite

show abstract

“…Thus, two-dimensional gel electrophoresis has been used to compare the protein profiles of lymph node tissue in MCL patients and normal control samples (15). A protein (antibody) microarray study compared proteins in CD19 ϩ purified B cells obtained from normal tonsils and histologically confirmed MCL patients (16).…”

mentioning

confidence: 99%

Protein Profiling of Plasma Membranes Defines Aberrant Signaling Pathways in Mantle Cell Lymphoma

Boyd

Jukes-Jones

Walewska

et al. 2009

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

“…This is in contrast to methods where whole tissue proteomes are analyzed using 2D gel in combination with MALDI-TOF analysis for protein identification. Thus, in some studies, like for many gene expression analyses, it is the composition of different cell types and their protein expression that is analyzed rather than differences between individual malignant and non-malignant cells (24,37).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly whole-proteome analysis, based on 2D gels and MALDI-TOF MS, was used to analyze biopsies from two MCL tumors, and nine proteins were found to be up-regulated in MCL compared with reactive lymph nodes (24). These approaches to the analysis of differences in protein content in malignant and non-malignant samples are based on a low number of analytes in comparison with gene expression profiling where Ͼ45,000 transcripts can be screened using e.g.…”

Section: Mantle Cell Lymphoma (Mcl)mentioning

confidence: 99%

From Gene Expression Analysis to Tissue Microarrays

Andréasson

Hober

et al. 2006

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCLassociated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues.

show abstract

Two‐dimensional molecular profiling of mantle cell lymphoma

Cited by 37 publications

References 35 publications

Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes

Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes

Protein Profiling of Plasma Membranes Defines Aberrant Signaling Pathways in Mantle Cell Lymphoma

From Gene Expression Analysis to Tissue Microarrays

Contact Info

Product

Resources

About