Two-dimensional proteomic analysis of gonads of air-breathing catfish, Clarias batrachus after the exposure of endosulfan and malathion

Laldinsangi, C.; Vijayaprasadarao, K.; Rajakumar, Arjuna; Murugananthkumar, Raju; Prathibha, Y.; Sudhakumari, C.C.; Mamta, Sajwan-Khatri; Dutta-Gupta, Aparna; Senthilkumaran, B.

doi:10.1016/j.etap.2014.03.007

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…15,16 Evaluating the adverse effects of pesticides on fish species has become very important due to the ecological and economic importance of fish. 17 Different toxic endpoints and different fish species have been employed to evaluate the toxicity of these pesticides. Previous literature reported the ability of these herbicides to damage liver cells and induce certain enzymatic responses in Clarias gariepinus, and also to disrupt normal fish behaviour and physiology under the same treatment conditions.…”

Section: Introductionmentioning

confidence: 99%

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Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat

2021

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The aquatic ecosystem is under increasing pressure from environmental contaminants due to anthropogenic activities. This study investigated the potential of glyphosate and paraquat to induce DNA damage and other cell abnormalities in juvenile African Catfish, Clarias gariepinus. Juvenile fish were exposed for 96 h to 0.36, 0.48, 0.60, 0.72 and 0.84 mg/L glyphosate; and 0.018, 0.037, 0.055, 0.110 and 0.221 mg/L paraquat. Following the exposure, the fish liver and blood were analysed for DNA damage and micronucleus respectively. DNA damage was analysed using comet assay while the micronucleus test was used for assessing nuclear abnormalities. Both herbicides induced DNA damage in fish, with paraquat exhibiting higher toxicity. The severity in liver DNA damage was observed to be dependent on concentration. The herbicides triggered formation of micronuclei, bean-shaped cells, lobed nuclei, and apoptosis in blood cells of fish. Both herbicides also increased the frequency of occurrence of these cell abnormalities in erythrocytes and showed mutagenic potential in fish. Glyphosate and Paraquat both have mutagenic potentials in fish and this is a reflection of the threat these contaminants pose to fish and other forms of aquatic life in our natural water bodies. Low concentrations of these herbicides should be encouraged when usage is inevitable

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Section: Introductionmentioning

confidence: 99%

“… 15 , 16 Evaluating the adverse effects of pesticides on fish species has become very important due to the ecological and economic importance of fish. 17 …”

Section: Introductionmentioning

confidence: 99%

Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat

2021

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“…Endosulfan is highly toxic to fish and aquatic invertebrates, and because of its lipophilicity, it is able to accumulate in these organisms (Howard, 1991;Hose and Van den Brink, 2004;Wan et al, 2005;Silva Barni et al, 2014). Also, endosulfan is an endocrine disrupting chemical that may affect reproductive parameters in animals as fishes, both in males and females (Chakravorty et al, 1992;Rajakumar et al, 2012;Laldinsangi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Histopathological effects of endosulfan to hepatopancreas, gills and ovary of the freshwater crab Zilchiopsis collastinensis (Decapoda: Trichodactylidae)

Negro

2015

Ecotoxicology and Environmental Safety

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“…As endocrine-disrupting chemicals (EDCs), the residues of malathion are often detected in natural environments and in food items as well (Kjeldsen et al 2013). They exert negative effects on the hematological system, immune response, and antioxidant enzyme activities at a low concentration (Laldinsangi et al 2014;Yonar et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Preparation of malathion MIP-SPE and its application in environmental analysis

Zuo

Zhu

Zhan

et al. 2015

Environ Monit Assess

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Malathion is an organophosphorous insecticide for controlling insects on fruits and vegetables, miscellaneous household insects, and animal parasites. It is important to develop highly efficient and selective pre-treatment method for analyzing malathion residues in environment and samples from agricultural products based on the molecularly imprinted polymers (MIPs). In this study, we developed a tailor-made MIP method with highly specific recognization to the template. The MIPs were prepared using malathion as a template, methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a crosslinker, azodiisobutyronitrile (AIBN) as an initiator, and the acetonitrile-chloroform (1:1, v/v) as a porogen. The molecular recognization mechanism of malathion and MAA was evaluated by molecular simulation, ultraviolet spectrometry (UV), and (1)H-nuclear magnetic resonance ((1)H-NMR). MAA interacted specifically with malathion by hydrogen bond with a ratio of 2:1. The MIPs exhibit a high affinity, recognition specificity, and efficient adsorption performance for malathion. The Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), surface area and porosimeter analyzer, thermogravimetric/differential thermal analyzer (TG/DTA) were used to characterize the properties of MIP. The malathion residues in soil, tap water, and cabbage were cleaned up by MIP-SPE, detected quantitatively using GC-FPD, and confirmed by GC-MS/MS. The limits of tap water, soil, and cabbage were confined to 0.001 mg L(-1), 0.004 and 0.004 mg kg(-1), respectively. The spiked recoveries of malathion were 96.06-111.49% (with RSD being 5.7-9.2%), 98.13-103.83% (RSD, 3.5-8.7%), and 84.94-93.69% (RSD, 4.7-5.8%) for tap water, soil, and cabbage samples, respectively. Thus, the method developed here can be used effectively in assessing malathion residues in multiple environmental samples. The aim of the study was to provide an efficient, selective, and accurate method for analyzing malathion at trace levels in multiple media.

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Two-dimensional proteomic analysis of gonads of air-breathing catfish, Clarias batrachus after the exposure of endosulfan and malathion

Cited by 9 publications

References 39 publications

Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat

Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat

Histopathological effects of endosulfan to hepatopancreas, gills and ovary of the freshwater crab Zilchiopsis collastinensis (Decapoda: Trichodactylidae)

Preparation of malathion MIP-SPE and its application in environmental analysis

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