Two-dimensional thermoresponsive sub-microporous substrate for accelerated cell tissue growth and facile detachment

Fragal, Vanessa H.; Catori, Daniele M.; Fragal, Elizângela H.; Garcia, Francielle P.; Nakamura, Celso Vataru; Rubira, Adley F.; Silva, Rafael

doi:10.1016/j.jcis.2019.03.071

Cited by 15 publications

(3 citation statements)

References 39 publications

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“…Thermoresponsive sub‐microporous substrate, such as poly( N ‐ isopropylacrylamide)/PS [ 145 ] and poly( N ‐isopropylacrylamide)/ formylated PS [ 146 ] with a lower critical solution temperature can be prepared by BF method. These materials have been applied to controlled growth of tissue cells, capture of nanoparticles, adjustable drug release and electrochemical application.…”

Section: Application Of Bf Membranementioning

confidence: 99%

Ordered Honeycomb‐Pattern Membrane^†

Yuan

Dai

et al. 2020

Chin. J. Chem.

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Ordered porous membranes have wide and important application prospects in the fields of filtration, separation, catalysis, biomedicine, etc., and have attracted growing interest in the field of material science. Among diverse membrane preparation approaches, breath pattern method has been widely concerned due to its simplicity in the preparation, ease in tuning the structure/property and functionality of formed membranes, and broad utility for various polymers. With the development of material design, the application spectrum of breath figure membrane has been greatly expanded. However, since the pore morphology of porous membrane is influenced by many factors, the controlling mechanism of normalization has not been clear yet. In this paper, the research progress of film-forming materials in recent years is reviewed, and the correlation between pore formation and pore morphology is discussed in details. This information will provide a systematic and in-depth perspective for research in this filed and an important guideline for the preparation of the ordered porous films with divergent applications. Hua Yuan (left) is an associate professor in College of Materials Science and Engineering, Qingdao University, China. She received her doctorate from Shandong University in China in 2013. She is mainly engaged in the research of carbon fiber, composites and microfiltration membrane. Guangzhen Li (middle) is a graduate student in the College of Materials Science and Engineering, Qingdao University, China. He graduated with a bachelor's degree in engineering from Qingdao University in 2019. His current research focuses on the structural design and functionalization of membrane materials. Enhao Dai (right) is a graduate student of the College of Materials Science and Engineering of Qingdao University. He received a bachelor's degree in engineering from Yantai University in 2018. Currently, he mainly studies carbon fiber modification.

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Section: Application Of Bf Membranementioning

confidence: 99%

Ordered Honeycomb‐Pattern Membrane^†

Yuan

Dai

et al. 2020

Chin. J. Chem.

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“…Introducing stimuli-responsiveness into porous hydrogels can endow these hydrogels with the prominent advantages of controllable drug delivery, adjustable pore diameter, and tunable ion absorption and cell concentration, which are significantly attractive in various applications [ 12 ]. There are many fundamental studies and applications of stimuli-responsive porous hydrogels in fields such as tissue engineering [ 13 ], self-healing [ 14 , 15 ], supercapacitors [ 16 ], 3D printing [ 17 , 18 ], and metal ion collection [ 19 ]. However, the low reliability, expensiveness, and low stimulus-response efficiency of these porous hydrogels still limit their application in the abovementioned fields.…”

Section: Introductionmentioning

confidence: 99%

Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network

et al. 2020

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Polystyrene nanospheres (PNs) were embedded in bovine skin gelatin gels with a poly(N-isopropylacrylamide) (PNIPAAm) network, which were denoted as NGHHs, to generate thermoresponsive behavior. When 265 nm PNs were exploited to generate the pores, bovine skin gelatin extended to completely occupy the pores left by PNs below the lower critical solution temperature (LCST), forming a pore-less structure. Contrarily, above the LCST, the collapse of hydrogen bonding between bovine skin gelatin and PNIPAAm occurred, resulting in pores in the NGHH. The behavior of pore closing and opening below and above the LCST, respectively, indicates the excellent drug gating efficiency. Amoxicillin (AMX) was loaded into the NGHHs as smart antibiotic gating due to the pore closing and opening behavior. Accordingly, E. coli. and S. aureus were exploited to test the bacteria inhibition ratio (BIR) of the AMX-loaded NGHHs. BIRs of NGHH without pores were 48% to 46.7% at 25 and 37 °C, respectively, for E. coli during 12 h of incubation time. The BIRs of nanoporous NGHH could be enhanced from 61.5% to 90.4% providing a smart antibiotic gate of bovine skin gelatin gels against inflammation from infection or injury inflammation.

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“…Polymer materials used as a platform for cell growth in general should have a surface suitable for cell adhesion and should not interact with the culture medium [22], and for that, during the cell culture assay, an important factor of the process is the culture medium, which is composed of salts enriched with amino acids and other components essential for cell growth.…”

Section: Introductionmentioning

confidence: 99%

Chelating Effect of Cellulose Acetate Hydrogel Crosslinked with EDTA Dianhydride Used as a Platform for Cell Growth

Melero

Senna

Domingues

et al. 2019

Advances in Materials Science and Engineering

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The use of biomaterials capable of achieving controlled release of drugs or chemical and physical interactions with living organisms causes these new materials to be studied in depth to better target their future applications. An example of this is the use of materials that have in their physical structure elements with chelating effects, capable of interacting with the ions and cations present in the cell culture media, drugs, and other elements capable of interacting with the human body. In this work, cellulose acetate hydrogel (HAC) crosslinked with EDTAD (ethylenediaminetetraacetic acid dianhydride) showed a significant chelating effect capable of altering the adhesion of mesenchymal cells in the first days of in vitro tests. This result became our main question in this work, and by using new cell viability assays, it was verified that the hydrogel interacted with the culture medium, removed the salts present, such as Ca2+ and Mg2+, and promoted a decrease in the amount of cells adhered to the material but not cell death. After saturation of Ca2+ and Mg2+, we obtained an improvement in cell adhesion. Electrochemical impedance spectroscopy (EIS) results also verified the dynamics of linked or mobile loads in the volume and interface regions of HAC-EDTA and the presence of characteristic bands related to the interaction between calcium and magnesium present in the culture medium with HAC-EDTA. The DMA traction test showed that there was no dynamic-mechanical change in the structure of the material at temperatures between 20 and 60°C, which is ideal for this studied biomaterial. The purpose of these tests is to aid in the characterization of future biomaterials, where the physicochemical interaction of some of them is often confused with a false cytotoxicity, which in fact may only be an easily identifiable and adaptable structural feature.

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Two-dimensional thermoresponsive sub-microporous substrate for accelerated cell tissue growth and facile detachment

Cited by 15 publications

References 39 publications

Ordered Honeycomb‐Pattern Membrane^†

Ordered Honeycomb‐Pattern Membrane^†

Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network

Chelating Effect of Cellulose Acetate Hydrogel Crosslinked with EDTA Dianhydride Used as a Platform for Cell Growth

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Two-dimensional thermoresponsive sub-microporous substrate for accelerated cell tissue growth and facile detachment

Cited by 15 publications

References 39 publications

Ordered Honeycomb‐Pattern Membrane†

Ordered Honeycomb‐Pattern Membrane†

Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network

Chelating Effect of Cellulose Acetate Hydrogel Crosslinked with EDTA Dianhydride Used as a Platform for Cell Growth

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Product

Resources

About

Ordered Honeycomb‐Pattern Membrane^†

Ordered Honeycomb‐Pattern Membrane^†