Two-dimensional transition metal dichalcogenides assisted biofunctionalized optical fiber SPR biosensor for efficient and rapid detection of bovine serum albumin

Abstract

The present study reports an alternative method of functionalizing the optical fiber Surface Plasmon Resonance (SPR) sensing probe with antibodies for label-free detection of bovine serum albumin (BSA) protein. In this novel approach, the gold coated fiber was first modified with Molybdenum disulfide (MoS 2 ) nanosheets followed by its bio-functionalization with Anti-BSA antibodies. The developed technique not only allowed the amplification of the SPR signals by synergic effects of MoS 2 and gold metallic thin film but also enabled a direct and chemical-free attachment of representative antibodies through hydrophobic interactions. The sensitivity of the MoS 2 modified sensing probe with detection limit of 0.29 µg/mL was improved as compared to the fiber optic SPR biosensor without MoS 2 overlayer (Detection limit for BSA was 0.45 μg/mL). The developed biosensor has good specificity, and environmental stability. Accordingly, the proposed design of the MoS 2 based SPR optical biosensor can offer the development of a simplified optical device for the monitoring of various biomedical and environmental parameters.

Exploring Graphene and MoS2 Chips Based Surface Plasmon Resonance Biosensors for Diagnostic Applications

“…In contrast, the limit of detection value on the MoS 2 -based SPR chip has a smaller value (0.29 μg/mL) when compared to conventional chips (0.45 μg/mL). These results confirm that the presence of MoS 2 on the SPR chip is proven to increase sensor sensitivity (Kaushik et al, 2019a ).…”

“…As a monolayer of MoS 2 possesses a higher optical absorption efficiency (~5%) than that of graphene (2.3%) (Lopez-Sanchez et al, 2013 ), it can promote plasmon excitation through an efficient charge transfer between MoS2 and the thin metallic film (Kim et al, 2019 ). In addition, the large surface area and the presence of free sulfur atoms are typical features of MoS 2 , which make it a potential material for developing biosensing interfaces (Kaushik et al, 2019a ). When MoS 2 layers are deposited on metal thin films, the strong coupling can be induced at the metal/MoS 2 interface because of the effective charge transfer and large electric field enhancement, which will result in increased SPR sensitivity (Hu et al, 2019 ).…”

“…In the same year, Kaushik et al ( 2019a ) investigated the effect of MoS 2 on the gold surface using optical fiber-based SPR biosensor to study interactions between anti-BSA and BSA. Experiments were carried out by injecting BSA with different concentrations (10–50 μg/mL) on conventional chip and MoS 2 -based chip.…”

“…Calibration curve of (B) Ab/gold/fiber; (D) Ab/MoS 2 /gold/fiber against varying concentration of BSA in PBS solution. Figures (A–D) were reproduced from Kaushik et al ( 2019a ) with permission from the Springer Nature.…”

Black Phosphorus All‐Fiber Sensor for Highly Responsive Humidity Detection

“…The combination of 2D sensing materials, such as graphene or TMDs, with different types of optical fibers for high‐performance vapor/gas sensor has been successfully demonstrated . In comparison, there have been few reports on BP‐based vapor/gas fiber sensors, though this strategy can provide excellent sensing performance by combining the beneficial characteristics from both BP and fiber optics .…”