Two-Dimensional Ultra-Thin-Layer Chromatography and Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry in Bioanalysis

Salo, Piia; Vilmunen, Suvi; Salomies, Hannele; Ketola, Raimo A.; Kostiainen, Risto

doi:10.1021/ac0620359

Cited by 49 publications

(24 citation statements)

References 37 publications

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“…Ultra-thin layer chromatography (UTLC) can also be combined with MS. UTLC is performed on 10 lm non-particulate planar stationary phases. Claimed benefits encompass faster development (1-6 min) and less solvent consumption (1-4 mL) at a similar or slightly lower resolution than with HPTLC (Salo 2007;Salo et al 2007). Although--as far as we know--this technique has not yet been applied to natural products, it seems worthwhile to explore its usefulness in phytochemical analysis.…”

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confidence: 99%

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

et al. 2009

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Recent developments in the rapid analysis of plants and tracking their bioactive constituents

et al. 2009

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“…Many classes of compounds have so far been analyzed by TLC with direct on-plate (MA)LDI-MS detection, including dyes (Gusev et al, 1995a), products of various organic reactions (St Hilaire et al, 1998), metal complexes (Matsumoto et al, 2002), siderophores from bacterial samples (Hayen & Volmer, 2005), pharmaceuticals and their impurities (Mowthorpe et al, 1999;Crecelius et al, 2002Crecelius, Clench, & Richards, 2004a;Salo et al, 2007), peptides (Gusev et al, 1995a;Mehl & Hercules, 2000), proteins (Gusev et al, 1995b;Bakry et al, 2007), nucleotides (Isbell et al, 1999), polymers (Matsumoto et al, 1999), as well as gangliosides (Ivleva et al, 2004(Ivleva et al, , 2005Dreisewerd et al, 2005), glycosphingolipids (GSLs) (Guittard, Hronowski, & Costello, 1999;Distler et al, 2008), LAB-ON-A-PLATE & phospholipids (Fuchs et al, 2007;Rohlfing et al, 2007), stem cell lipids (Fuchs et al, 2008), and bacterial toxins (Meisen et al, 2009). An example is shown in Figure 20.…”

Section: On-plate Separationmentioning

confidence: 99%

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Urban

Amantonico

Zenobi

2011

Mass Spectrometry Reviews

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We review the literature that describes how (matrix-assisted) laser desorption/ionization (MA)LDI target plates can be used not only as sample supports, but beyond that: as functional parts of analytical protocols that incorporate detection by MALDI-MS or matrix-free LDI-MS. Numerous steps of analytical procedures can be performed directly on the (MA)LDI target plates prior to the ionization of analytes in the ion source of a mass spectrometer. These include homogenization, preconcentration, amplification, purification, extraction, digestion, derivatization, synthesis, separation, detection with complementary techniques, data storage, or other steps. Therefore, we consider it helpful to define the "lab-on-a-plate" as a format for carrying out extensive sample treatment as well as bioassays directly on (MA)LDI target plates. This review introduces the lab-on-plate approach and illustrates it with the aid of relevant examples from the scientific and patent literature.

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“…Unfortunately, AP-MALDI possesses normally lower sensitivity than common high vacuum MALDI. Nevertheless, it was shown that AP-MALDI in combination with UTLC is sufficiently sensitive to detect benzodiazepines directly in human urine without the need of a major workup of the sample (Salo et al 2007). One interesting application is the investigation of changes of dyes during photo-aging, i.e., upon the light-induced bleaching process.…”

Section: Pharmaceuticalsmentioning

confidence: 99%

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

Schiller

Fuchs

Teuber

et al. 2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Thin-layer chromatography (TLC) is a widely used, fast and inexpensive method. Even minor constituents of complex mixtures can be often characterized. Unfortunately, the unambiguous assignment of the spots to defined compounds is often difficult because common staining methods normally reveal only (at best) a substance class, not a specific compound. For instance, in the case of phospholipids, the lipid class, but not the detailed fatty acyl composition, can be determined (normal phase TLC). Nowadays mass spectrometry (MS) seems to be the most suitable method for analyte characterization due to its high sensitivity and mass accuracy. This became possible by the invention of soft ionization methods such as matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) MS and electrospray ionization (ESI) although many further desorption techniques are available nowadays. This review summarizes the so far available knowledge about direct TLC/MS couplings and gives an overview about selected compounds that could be successfully analyzed. Chromatographic Methods and Product IdentificationLiquid chromatographic methods, especially high-performance liquid chromatography (HPLC) and planar chromatography [in particular thin-layer (TLC) or highperformance thin-layer chromatography (HPTLC)], are indispensable tools of modern analytical chemistry (Hahn-Deinstrop 2006 l TLC is a very robust system and to a much lesser extent affected by impurities potentially present in the sample. Thus, TLC may also be used for the analysis of "suspicious" samples (for instance, crude tissue or food extracts) that might easily damage or plug the column of an HPLC system. l HPLC columns are normally rather expensive and are, thus, many times reused subsequent to careful washing to remove previous analytes. However, it can never be completely ruled out that there is some residual material on the column. Thus, it is often more difficult to certify an HPLC assay than a TLC assay because TLC does not provide any "memory" effects as a completely new stationary phase is used in all cases. The consumption of solvents is much lower than in the case of HPLC. This makes TLC less expensive and particularly less harmful and more environment friendly.l Although nowadays there are also "multiplexing" HPLC systems available (Woodbury et al. 1995) that allow the simultaneous analysis of several samples in parallel, the simple analysis of a multitude of samples is also a clear advantage of TLC.There is hardly a class of small molecules that have not yet been analyzed by TLC, and thus this review will not deal with improvements of the achievable separation quality, neither by optimizing the stationary phase nor the solvent system nor sample application.However, separation is only one (unequivocally very important) step of successful analysis, and the detection of the different analytes on the TLC plate and their structural elucidation is also very important. This particularly holds because the retardation factor (R f value) by which a com...

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Two-Dimensional Ultra-Thin-Layer Chromatography and Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry in Bioanalysis

Cited by 49 publications

References 37 publications

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

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