Two distinct Ca2+ compartments show differential sensitivity to thrombin, ADP and vasopressin in human platelets

López, José J.; Redondo, Pedro C.; Salido, Ginés M.; Pariente, José A.; Rosado, Juan A.

doi:10.1016/j.cellsig.2005.05.006

Cited by 93 publications

(89 citation statements)

References 31 publications

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“…EDTA was also used in our own experiments. ADP releases Ca 2ϩ only from the DTS 23 ; thus, modeling the acidic store is not required at this point. Figure 2E) was modeled according to a kinetic study of the type 3 SERCA isoform, 24 which is abundant in human platelets.…”

Section: Model Overviewmentioning

confidence: 99%

A molecular signaling model of platelet phosphoinositide and calcium regulation during homeostasis and P2Y1 activation

Purvis

Chatterjee²,

Brass

et al. 2008

Blood

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Section: Model Overviewmentioning

confidence: 99%

A molecular signaling model of platelet phosphoinositide and calcium regulation during homeostasis and P2Y1 activation

Purvis

Chatterjee²,

Brass

et al. 2008

Blood

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“…In a Ca 2ϩ -free medium (100 mol/L of ethylene glycol tetraacetic acid [EGTA] was added), treatment of platelets with thrombin (Thr; 0.1 U/mL) evokes a rapid and transient increase in [Ca 2ϩ ] c as the result of Ca 2ϩ release from the intracellular stores, the dense tubular system, and the acidic stores, 18 followed by a Ca 2ϩ clearance through Ca 2ϩ -ATPases. As shown in Figure 1, Ca 2ϩ mobilization evoked by Thr was significantly increased by 22.7Ϯ12.6% (meanϮSEM) after incubation for 5 minutes at 37°C with 50 mol/L of CsA (Figure 1, inset; PϽ0.05; nϭ5).…”

Section: Csa Increases Thrombin-evoked Ca 2؉ Mobilizationmentioning

confidence: 99%

“…18,19 Both SERCAs can be specifically inhibited by using either a low concentration of thapsigargin (TG) or 2,5-di-(tert-butyl)-1,4-hydroquinone (TBHQ), respectively. 20 -21 To investigate the effect of CsA on SERCA2b, we treated the cells with Thr, 0.1 U/mL, in combination with TBHQ, 20 mol/L.…”

Section: Csa Reduces Ca 2؉ Reuptake By Serca2b In Human Plateletsmentioning

confidence: 99%

“…with thrombin (Thr; 0.1 U/mL) evokes a rapid and transient increase in [Ca 2ϩ ] c as the result of Ca 2ϩ release from the intracellular stores, the dense tubular system, and the acidic stores, 18 followed by a Ca 2ϩ clearance through Ca 2ϩ -ATPases. As shown in Figure 1, Ca 2ϩ mobilization evoked by Thr was significantly increased by 22.7Ϯ12.6% (meanϮSEM) after incubation for 5 minutes at 37°C with 50 mol/L of CsA (Figure 1, inset 18,19 Both SERCAs can be specifically inhibited by using either a low concentration of thapsigargin (TG) or 2,5-di-(tert-butyl)-1,4-hydroquinone (TBHQ), respectively. 20 -21 To investigate the effect of CsA on SERCA2b, we treated the cells with Thr, 0.1 U/mL, in combination with TBHQ, 20 mol/L.…”

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confidence: 99%

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SERCA2b Activity Is Regulated by Cyclophilins in Human Platelets

Rosado

Pariente

Salido

et al. 2010

ATVB

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Objective-The role of cyclophilins (chaperones that are widely expressed in different cell types, including human platelets) was explored in sarcoendoplasmic calcium (Ca 2ϩ ) adenosine triphosphatase (SERCA) activity. Methods and Results-Cyclophilin inhibition by cyclosporin A (CsA) evoked a time-and concentration-dependent reduction of Ca 2ϩ uptake by SERCA2b. However, other Ca 2ϩ -adenosine triphosphatases expressed in platelets, such as SERCA3 and plasma membrane Ca 2ϩ adenosine triphophatase, remained unaltered after CsA treatment. Cypermethrin, a non-CsA-related calcineurin inhibitor, did not alter SERCA2b activity. Furthermore, SERCA2b was affected by other CsA analogues, which do not interfere with calcineurin, such as PKF-211-811-NX5 (NIM811) and sanglifehrin A. Inhibition of the immunophilin family members using FK506 (tacrolimus) did not alter SERCA2b ability to sequester Ca 2ϩ into the dense tubular system. Coimmunoprecipitation experiments confirmed that cyclophilin A associates with SERCA2b and stromal interaction molecule-1 in resting platelets. This interaction is attenuated by the physiological agonist thrombin but enhanced by treatment with CsA or sanglifehrin A. T he immunophilin family groups proteins with rotenase or peptidylprolyl cis-transisomerase activity. 1,2 These proteins have been classified according to their sensitivity to specific immunosuppressor drugs: cyclophilins (CyPs) are specific targets of cyclosporin A (CsA) [1][2][3][4] ; immunophilins (FKBPs) are sensitive to FK506 (tacrolimus) and rapamycin (sirolimus), both structurally unrelated to CsA 4 -6 ; and FCBPs are sensitive to CsA and FK506. 2 CyPs are involved in two major cellular events: (1) indirect and nonspecific regulation of serine-threonine kinase proteins, an event mediated by the formation of stable CyP/CsA complexes that interact with the regulatory center of calcineurin (CaN), PP2B, or calcium (Ca 2ϩ )/calmodulin kinase (Ca 2ϩ /CaMK) phosphatase, reducing its activity 7 ; and (2) control of cell death by apoptosis through CyP D, which participates in the formation of the mitochondrial permeability transition pore and cytochrome c release in apoptotic cells. Furthermore, CyP B suppresses apoptosis evoked by oxidative, and endoplasmic reticulum, stress. 8 -10 Immunophilins participate in Ca 2ϩ homeostasis in several cell models. However, this role has been mainly attributed to FKBPs that control the opening of Ca 2ϩ channels located in the endoplasmic reticulum, such as ryanodine or inositol 1,4,5-trisphosphate receptors. [11][12][13][14] In contrast, little is known about the role of immunophilins on Ca 2ϩ reuptake or extrusion mechanisms, and the current studies [13][14][15][16] have reported contradictory results about the role of CyPs on the activity of sarcoendoplasmic Ca 2ϩ adenosine triphosphatase (ATPase) (SERCA) or the plasma membrane Ca 2ϩ ATPase (PMCA). In the present study, we have investigated the role of CyPs in the regulation of Ca 2ϩ reuptake by SERCA. We found that CsA reduces Ca 2ϩ reuptake by...

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“…Among other differences in platelets is a different topology, peripheral for SERCA3, more central for SERCA2b. 24 SERCA3 is specifically associated with acidic Ca 21 stores, 25 and with STIM1 (the Ca 21 sensor of the store operated Ca 21 entry [SOCE]). 26 To assess the role of SERCA3 in platelets, we have decided to assess the hemostasis status of SERCA3 2/2 mice.…”

Section: /2mentioning

confidence: 99%

Full activation of mouse platelets requires ADP secretion regulated by SERCA3 ATPase–dependent calcium stores

Elaib

Adam

Berrou

et al. 2016

Blood

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Two distinct Ca2+ compartments show differential sensitivity to thrombin, ADP and vasopressin in human platelets

Cited by 93 publications

References 31 publications

A molecular signaling model of platelet phosphoinositide and calcium regulation during homeostasis and P2Y1 activation

A molecular signaling model of platelet phosphoinositide and calcium regulation during homeostasis and P2Y1 activation

SERCA2b Activity Is Regulated by Cyclophilins in Human Platelets

Full activation of mouse platelets requires ADP secretion regulated by SERCA3 ATPase–dependent calcium stores

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