Two Distinct Human Uterine Cervical Epithelial Cell Lines Established after Transfection with Human Papillomavirus 16 DNA

Ohta, Y.; Tsutsumi, Kouichiro; Kikuchi, Keiji; Yasumoto, Shigeru

doi:10.1111/j.1349-7006.1997.tb00432.x

Cited by 8 publications

(10 citation statements)

References 21 publications

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“…Normal human cervical keratinocytes (NHCK), immortalized with HPV 16 [20], were cultured in serum-free MCDB153 medium (Sigma-Aldrich, St. Louis, MO) supplemented with sodium bicarbonate (1.2 g/l), insulin (5 μg/ml), hydrocortisone (0.5 μg/ml), transferrin (10 μg/ml), O -phosphoethanolamine (0.1 mM), ethanolamine (0.1 mM), epidermal growth factor (10 ng/ml) and bovine pituitary extract (40–70 μg/ml) (all from Sigma-Aldrich). BPDE (CAS no.…”

Section: Participants and Methodsmentioning

confidence: 99%

Quantification of phase I/II metabolizing enzyme gene expression and polycyclic aromatic hydrocarbon–DNA adduct levels in human prostate

et al. 2009

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Section: Participants and Methodsmentioning

confidence: 99%

Quantification of phase I/II metabolizing enzyme gene expression and polycyclic aromatic hydrocarbon–DNA adduct levels in human prostate

et al. 2009

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“…They can be multiplied over several months in vitro, retaining speci®c cellular phenotypes that re¯ect their cellular origins (Tsutsumi et al, 1993;Ohta et al, 1997). The¯ow-cytometric analysis shows that almost all cells in the explant cultures are positive for EGFR and integrins, suggesting that the cell population predominantly contains cell fractions derived from basal and suprabasal layers ( Figure 3A).…”

Section: Not All Basal Cells Have Telomerase Activitymentioning

confidence: 99%

Telomerase activity in a specific cell subset co-expressing integrinβ1/EGFR but not p75NGFR/bcl2/integrin β4 in normal human epithelial cells

Kikuchi

Ahmed

et al. 1998

Oncogene

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Telomerase activity is correlated with the immortality of various cultured cells and cancer cells. The activity, however, is also demonstrated in various normal regenerating cells which normally have a ®nite life span in vivo and in vitro, though its biological implication remains unclear. Using cultured normal human epithelial cells, we show that telomerase activity is associated with epithelial cell subsets which actively proliferate in vitro. Unlike in most cancer cell lines, telomerase activity was evidently up-regulated when the cells entered into S phase in primary human epithelial cells. To characterize the cells which have telomerase activity, the primary human epithelial cell population of uterine cervix was dissociated into several distinctive cellular subsets by means of immunocytochemical cell fractionation. Telomerase activity was found to be closely associated with the subset which expressed predominantly integrin b1 and epidermal growth factor receptor. We further identi®ed the telomerase-negative subpopulation which contained a small subset that strongly coexpresses p75 NGFR low a nity nerve growth factor receptor, integrin b4 and bcl-2 protein. The location of the p75 NGFR -expressing cells contrasts to that of the Ki-67 positive cells in vivo and is distinctive of telomerase positive cycling cells, indicating that these cells remain at the G0 phase. The present study supports the notion that telomerase activity is linked to cell cycle regulation, implying that telomerase is activated upon cell proliferation in regenerating normal human epithelial cells.

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“…NCE16N þ is an HPV16-immortalized cell line of ectocervical epithelial cell origin strongly expressing p75 NTR . The characteristics of this cell line have been described previously (Ohta et al, 1997;Kunimura et al, 1998).…”

Section: Preparation Of Normal Human Epithelial Cellsmentioning

confidence: 99%

Neurotrophin receptor p75NTR characterizes human esophageal keratinocyte stem cells in vitro

et al. 2003

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We report here that human esophageal keratinocyte stem cells are characterized by the expression of the lowaffinity neurotrophin receptor p75 NTR and differentially expressed cell adhesion molecules, the b1 and b4 integrins. The candidate stem cells could be fractionated from keratinocytes as a minor cell subset by means of immunocytochemical cell sorting based on the different levels of expression of these cell surface molecules. Flow cytometric analysis revealed that this minor cell subset retained a relatively slow-cycling phenotype in vitro. These cells expressed low levels of involucrin and cytokeratin 13, indicating that the p75 NTR -positive cell subset is immature relative to the other predominant subpopulations coexpressing b1 integrin at higher levels. The p75 NTR -positive cell subset was crucial for achieving longevity and the greatest output of keratinocytes comprising all distinguishable subpopulations in vitro. This process was associated with self-renewal and selfamplification of the p75 NTR -positive cell subset. These findings strongly implicate p75 NTR as a stem cell marker, which will be valuable for prospectively investigating stem cell regulation in association with different biological processes including neoplastic transformation of regenerative epithelia.

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Two Distinct Human Uterine Cervical Epithelial Cell Lines Established after Transfection with Human Papillomavirus 16 DNA

Cited by 8 publications

References 21 publications

Quantification of phase I/II metabolizing enzyme gene expression and polycyclic aromatic hydrocarbon–DNA adduct levels in human prostate

Quantification of phase I/II metabolizing enzyme gene expression and polycyclic aromatic hydrocarbon–DNA adduct levels in human prostate

Telomerase activity in a specific cell subset co-expressing integrinβ1/EGFR but not p75NGFR/bcl2/integrin β4 in normal human epithelial cells

Neurotrophin receptor p75NTR characterizes human esophageal keratinocyte stem cells in vitro

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