Two distinct interferon-γ genes in Tetraodon nigroviridis : Functional analysis during Vibrio parahaemolyticus infection

Wan, Peng; Lu, Danqi; Li, Gaofei; Zhang, Xu; Yao, Mi; Zhang, Yong; Lin, Haoran

doi:10.1016/j.molimm.2015.12.004

Cited by 28 publications

(12 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For IFNγ, it was found that the unglycosylated IFNγ suffered from shorter half-life and lower protease resistance, while its function seemed to be unchanged ( 42 ). Functional in vitro assays exhibited that r Ec IFNγ1 and r Ec IFNγ2 could induce nitric oxide response, which effects are similar with the previous researches from mammals, goldfish, tetraodon, and common carp, in which, IFNγ were found to induce a nitric oxide response ( 5 , 6 , 8 , 39 , 43 ). Meanwhile, both r Ec IFNγ1 and r Ec IFNγ2 significantly enhanced the respiratory burst response, which effects were also observed in mammalian, goldfish and rainbow trout IFNγs ( 5 – 7 , 44 ).…”

Section: Discussionsupporting

confidence: 87%

“…Our previous study in T. nigroviridis indicated that although T. nigroviridis IFNγs were not able to protect T. nigroviridis from Vibrio parahaemolyticus infection, T. nigroviridis IFNγ1 may promote an overwhelming inflammatory response, whereas T. nigroviridis IFNγ2 suppressed the inflammatory reaction promoted by V. parahaemolyticus infection ( 8 ). These further supported our speculation that r Ec IFNγ2 would regulate immune response by IFNγ2-miR-146a-TRAF6 negative regulation loop (Figure 10 ).…”

Section: Discussionmentioning

confidence: 99%

“…Primary blood lymphocytes cultivation and in vitro stimulation were performed as described in Nitric oxide assay. The respiratory burst assay was performed as previously described ( 8 ). These cells were incubated at 28°C in 5% CO 2 for 18 h. Then, NBT (2 mg/mL, Sigma) and PMA (final concentration, 100 ng/mL, Sigma) were added to the cell cultures at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Only one single IFNγ gene is identified in mammalian ( 1 ), avian ( 2 ), and amphibian ( 3 ) species, while many fishes possess two IFNγ genes, namely IFNγ1 and IFNγ2. Like mammalian IFNγ, teleost IFNγs mediate their protective effects as an activator of macrophages, through the enhancing of respiratory burst activity, nitric oxide production, and bacterial phagocytosis ( 4 – 8 ), inducing of the expression of pro-inflammatory cytokines ( 4 , 5 , 9 ) and the typical antiviral genes ( 8 , 10 ). Respiratory burst and nitric oxide are well known as potent antimicrobials ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

“…IFNγ was produced by natural killer and T cells during the recognition of pathogens by TLRs ( 15 ). Like mammalian IFNγ ( 16 ), teleost IFNγs also play various roles in response to bacterial or viral infection ( 8 , 9 , 17 , 18 ). IFNγ and TLR signaling pathways are important for both innate and adaptive immune responses.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Two Distinct Interferon-γ in the Orange-Spotted Grouper (Epinephelus coioides): Molecular Cloning, Functional Characterization, and Regulation in Toll-Like Receptor Pathway by Induction of miR-146a

Wan

Sun

et al. 2018

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

Interferon gamma (IFNγ) is a Th1 cytokine that is critical for innate and adaptive immunity. Toll-like receptors (TLRs) signaling pathways are critical in early host defense against invading pathogens. miR-146a has been reported to participate in the regulation of host immunity. The known mechanisms of integrations between the IFNγ and TLR signaling pathways are incompletely understood, especially in teleosts. In this study, orange-spotted grouper (Epinephelus coioides) IFNγ1 and IFNγ2, their biological activities, especially their involvements in TLR pathway, were explored. We identified and cloned two IFNγ genes of E. coioides, namely EcIFNγ1 and EcIFNγ2. The produced recombinant E. coioides IFNγ1 (rEcIFNγ1) and IFNγ2 (rEcIFNγ2) proteins showed functions, which are similar to those of other bony fishes, such as enhancing nitric oxide responses and respiratory burst response. rEcIFNγ2 could regulate TLR pathway by enhancing the promoter activity of miR-146a upstream sequence and thus increasing the expression level of miR-146a, which possibly targets TNF receptor-associated factor 6 (TRAF6), a key adapter molecule in TLR signaling pathway. Taken together, these findings unravel a novel regulatory mechanism of anti-inflammatory response by IFNγ2, which could mediate TLR pathway through IFNγ2–miR-146a–TRAF6 negative regulation loop. It is suggested that IFNγ2 may provide a promising therapeutic, which may help to fine tune the immune response.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Two Distinct Interferon-γ in the Orange-Spotted Grouper (Epinephelus coioides): Molecular Cloning, Functional Characterization, and Regulation in Toll-Like Receptor Pathway by Induction of miR-146a

Wan

Sun

et al. 2018

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

show abstract

Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway

Shibasaki,

Yabu,

Shiba

et al. 2024

FEBS Open Bio

View full text Add to dashboard Cite

Unlike mammals, fish express two type II interferons, IFNγ and fish‐specific IFNγ (IFNγ‐related or IFNγrel). We previously reported the presence of two IFNγrel genes, IFNγrel 1 and IFNγrel 2, which exhibit potent antiviral activity in the Ginbuna crucian carp, Carassius auratus langsdorfii. We also found that IFNγrel 1 increased allograft rejection; however, the IFNγrel 1 receptor(s) and signaling pathways underlying this process have not yet been elucidated. In this study, we examined the unique signaling mechanism of IFNγrel 1 and its receptors. The phosphorylation and transcriptional activation of STAT6 in response to recombinant Ginbuna IFNγrel 1 (rgIFNγrel 1) was observed in Ginbuna‐derived cells. Binding of rgIFNγrel 1 to Class II cytokine receptor family members (Crfbs), Crfb5 and Crfb17, which are also known as IFNAR1 and IFNGR1‐1, respectively, was detected by flow cytometry. Expression of the IFNγrel 1‐inducible antiviral gene, Isg15, was highest in Crfb5‐ and Crfb17‐overexpressing GTS9 cells. Dimerization of Crfb5 and Crfb17 was detected by chemical crosslinking. The results indicate that IFNγrel 1 activates Stat6 through an interaction with unique pairs of receptors, Crfb5 and Crfb17. Indeed, this cascade is distinct from not only that of IFNγ but also that of known IFNs in other vertebrates. IFNs may be classified by their receptor and signal transduction pathways. Taken together, IFNγrel 1 may be classified as a novel type of IFN family member in vertebrates. Our findings provide important information on interferon gene evolution in bony fish.

show abstract

Growth hormone secretagogue peptide A233 upregulates Mx expression in teleost fish in vitro and in vivo

Martı́nez¹,

Fernández-Trujillo

Hernández³

et al. 2022

Arch Virol

View full text Add to dashboard Cite

Aquaculture constitutes an alternative source for food production and contributes to the reduction of indiscriminate catch of aquatic organisms from their natural environment (Diaz & Neira, 2005;Beveridge et al., 2013). However, high mortality during larval state remains a challenge in this sector, mainly because of factors like diets and diseases induced by pathogens (Helvik et al., 2009;Stentiford et al., 2017). Therefore, growth and health management is a key strategy for sustainable aquaculture (Martinez et al., 2016a).

show abstract

Two distinct interferon-γ genes in Tetraodon nigroviridis : Functional analysis during Vibrio parahaemolyticus infection

Cited by 28 publications

References 58 publications

Two Distinct Interferon-γ in the Orange-Spotted Grouper (Epinephelus coioides): Molecular Cloning, Functional Characterization, and Regulation in Toll-Like Receptor Pathway by Induction of miR-146a

Two Distinct Interferon-γ in the Orange-Spotted Grouper (Epinephelus coioides): Molecular Cloning, Functional Characterization, and Regulation in Toll-Like Receptor Pathway by Induction of miR-146a

Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway

Growth hormone secretagogue peptide A233 upregulates Mx expression in teleost fish in vitro and in vivo

Contact Info

Product

Resources

About