Two homologous carboxylesterase genes from Locusta migratoria with different tissue expression patterns and roles in insecticide detoxification

Zhang, Jianqin; Ge, Pingting; Li, Daqi; Guo, Yaping; Zhu, Kun Yan; Ma, Enbo; Zhang, Jianzhen

doi:10.1016/j.jinsphys.2015.03.013

Cited by 30 publications

(28 citation statements)

References 40 publications

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“…Thus, the enhanced activity of P450 enzymes can also explain the cross‐resistance between sulfoxaflor and other neonicotinoid insecticides in the current study. The enhanced detoxification by EST in insects has been reported to be associated with metabolic resistance to organophosphates and carbamates . Sun et al .…”

Section: Discussionmentioning

confidence: 99%

“…For insecticide bioassays after RNAi, 30 fifth-instar nymphs were collected 24 h after injection and transferred onto rice seedlings that had been treated with a solution of 320 mg L −1 (concentration between the LC 30 and LC 40 values of sulfoxaflor to the fifth-instar nymphs of G 39 ) sulfoxaflor as described above. Insects injected with dsGFP were used as the controls.…”

Section: Rna Interference and Bioassaysmentioning

confidence: 99%

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Characterization of sulfoxaflor resistance in the brown planthopper, Nilaparvata lugens (Stål)

Liao

Jin

Zhang

et al. 2019

Pest Management Science

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BACKGROUND Sulfoxaflor is a new insecticide for controlling Nilaparvata lugens in the field. This study was conducted to investigate the risk of resistance development, the cross‐resistance spectrum and the mechanisms of sulfoxaflor resistance in N. lugens. RESULTS A sulfoxaflor‐resistant strain was obtained from a field population by successive selection with sulfoxaflor for 39 generations in the laboratory. Sulfoxaflor‐resistant populations showed significant levels of cross‐resistance to dinotefuran, nitenpyram, thiamethoxam, clothianidin, imidacloprid and cycloxaprid. However, they exhibited only minor or no cross‐resistance to isoprocarb, etofenprox, chlorpyrifos, triflumezopyrim and buprofezin. Sulfoxaflor was synergized by the inhibitor piperonyl butoxide (PBO) in the sulfoxaflor‐resistant strain (SFX‐SEL) with 2.69‐fold relative synergistic ratios compared with the unselected strain (UNSEL). Compared with UNSEL, the P450 enzyme activity of SFX‐SEL was increased 3.50 times, and eight P450 genes were upregulated more than 2.0‐fold in SFX‐SEL. RNAi reduced the expression of CYP6ER1 (36.87‐fold change) and significantly enhanced the susceptibility of SFX‐SEL to sulfoxaflor. CONCLUSION Resistance development and cross‐resistance risk of sulfoxaflor‐resistance in N. lugens is evident. The enhanced detoxification of P450 enzymes caused by upregulation of several P450 genes is considered to be the metabolic resistance mechanism. These results suggest that CYP6ER1 might play an important role in sulfoxaflor resistance in N. lugens. © 2018 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Rna Interference and Bioassaysmentioning

confidence: 99%

Characterization of sulfoxaflor resistance in the brown planthopper, Nilaparvata lugens (Stål)

Liao

Jin

Zhang

et al. 2019

Pest Management Science

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“…Expression of detoxification enzymes in insects is induced by various xenobiotic molecules such as plant allelochemicals (including volatiles), mycotoxins and insecticides (Li et al, 2002, Ranson et al, 2002Li et al, 2007;Deng et al, 2009;Von Mérey et al, 2013;Zeng et al, 2013;Guo et al, 2015;Liang et al, 2015;Zhang et al, 2015;Wang et al, 2017). Insecticides are currently indispensable for S. litura control of almost all attacked crops.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, GSTs in insects are involved in inactivation of endogenous metabolites and detoxification of xenobiotics (Feyereisen, ; Deng et al ., ). CarEs belong to a superfamily of multifunctional and ubiquitous enzymes which play important roles in many metabolic reactions related to cell development such as neurogenesis (Zhang et al ., ; Salim et al ., ). It has been reported that insect CarEs could be involved in resistance to many insecticides, such as organophosphates, carbamates and pyrethroids (Zhang et al ., ; Salim et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…CarEs belong to a superfamily of multifunctional and ubiquitous enzymes which play important roles in many metabolic reactions related to cell development such as neurogenesis (Zhang et al ., ; Salim et al ., ). It has been reported that insect CarEs could be involved in resistance to many insecticides, such as organophosphates, carbamates and pyrethroids (Zhang et al ., ; Salim et al ., ). Acetylcholinesterase (AChE), a key enzyme of the insect nerve system, is an important target of two major insecticide families, organophosphates and carbamates (Salim et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Exposure to herbicides reduces larval sensitivity to insecticides in Spodoptera litura (Lepidoptera: Noctuidae)

et al. 2018

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Herbicides and insecticides are widely used in modern agriculture. It has been reported in various studies that application of insecticides can increase tolerance of herbivorous insects to insecticides. However, limited information exists on susceptibility to insecticides when insects are exposed to herbicides. This study was conducted to investigate the potential impact of the herbicides trifluralin and 2‐methyl‐4‐chlorophenoxyacetic acid sodium salt (MCPA‐Na) on the susceptibility of the nocturnal moth Spodoptera litura to the insecticides λ‐cyhalothrin, phoxim and bifenthrin. We found that larvae exposed to trifluralin or MCPA‐Na became significantly less susceptible to both insecticides than non‐exposed control larvae. Herbicide‐treated larvae did not show altered growth under the used test conditions. However, heads of herbicide‐treated larvae showed increased expression of the acetylcholinesterase genes SlAce1 and SlAce2. Moreover, the fat body and midgut of herbicide‐treated larvae displayed elevated expression of detoxification genes (the carboxylesterase gene SlCarE; the glutathione S‐transferase genes SlGSTe2 and SlGSTe3; the cytochrome P450 monooxygenase genes CYP6B48, CYP9A40 and CYP321B1). The CYP6B48 gene exhibited highest inducibility. In conclusion, the data of this study suggest that exposure of S. litura larvae to herbicides may stimulate detoxification mechanisms that compromise the efficacy of insecticides.

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Decreased carboxylesterase expression associated with increased susceptibility to insecticide in Mythimna separata

Liu

Yang

et al. 2021

Arch Insect Biochem Physiol

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Carboxylesterases are one of the three major types of detoxification enzyme in insects. In this study, we screened 12 full‐length carboxylesterase cDNA sequences from the oriental armyworm Mythimna separata; they were named MsCarE1–MsCarE12 and registered in GenBank with accession numbers MK440541–MK440552. Treatment of fourth instar larvae of M. separata with the LD50 of the insecticide chlorantraniliprole increased the expression levels of MsCarE3 and MsCarE4, while treatment with the LD50 of lambda‐cyhalothrin significantly increased the expression levels of MsCarE5 and MsCarE10. Spatiotemporal expression detection showed that MsCarE3, MsCarE4, MsCarE5, and MsCarE10 were expressed at different developmental stages and in different tissues of M. separata and their expression levels were different. Induction using a high dose of chlorantraniliprole resulted in lower expression of MsCarE3 and MsCarE4. LD50 of lambda‐cyhalothrin induced higher expression of MsCarE5 and MsCarE10, while LD70 induced higher MsCarE10 expression at 3, 6, and 12 h after treatment. RNA interference successfully inhibited the expression of MsCarE3, MsCarE4, MsCarE5, and MsCarE10, to different degrees at different time points. Silencing of MsCarE5, or MsCarE5 and MsCarE10 simultaneously changed carboxylesterase activity and increased the susceptibility of M. separata larvae to lambda‐cyhalothrin. This study provides a new method to increase the insect susceptibility to insecticide.

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Two homologous carboxylesterase genes from Locusta migratoria with different tissue expression patterns and roles in insecticide detoxification

Cited by 30 publications

References 40 publications

Characterization of sulfoxaflor resistance in the brown planthopper, Nilaparvata lugens (Stål)

Characterization of sulfoxaflor resistance in the brown planthopper, Nilaparvata lugens (Stål)

Exposure to herbicides reduces larval sensitivity to insecticides in Spodoptera litura (Lepidoptera: Noctuidae)

Decreased carboxylesterase expression associated with increased susceptibility to insecticide in Mythimna separata

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