Two in vitro anthelmintic assays of four Paraguayan medicinal plants for proof of concept of the role of polyphenols in their biological activities and LC-HRMS analysis

Ocampos, Griselda Meza; Ñumbay, M Torres; Haddad, Mohamed; Ambassa, Lin Marcellin Messi; Alvarenga, Nelson; Hoste, Hervé

doi:10.1016/j.jep.2023.116453

Cited by 1 publication

(5 citation statements)

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“…Similar results have been recently published by the group of Zheleva-Dimitrova et al (2023). According to Ocampos et al (2023) the AH activity of A. absinthium, a medicinal plant of the Asteraceae family with deworming and insecticidal properties (Szopa et al, 2020), could be mainly attributed to its polyphenolic compounds, without dismissing the fact that other metabolites may also be present and play a crucial role. Among them, apigenin was one of the identified active flavones.…”

Section: Polyphenolic Contentsupporting

confidence: 82%

“…For the chemical characterization of the species, the primary focus was on STLs, caffeic acid derivatives, and polyphenols. During this analysis, we identified several substances that have previously demonstrated anthelmintic and insecticidal properties (Valente et al, 2021;Ocampos et al, 2023). Notably, ChA, 3.5 DCQ, GPL, CGA, Ap-7-O-gluc, Lu-7-O-gluc, Ap, and Lu were found to be the most abundant metabolites.…”

Section: Discussionmentioning

confidence: 88%

“…Flavones, such as Ap-7-O-gluc, Ap, Lu-7-O-gluc, and Lu, characteristic for Asteraceae, are also present. These metabolites are suspected to possess the main antiparasitic, antioxidant, and AH properties (Poulopoulou et al, 2022., Zheleva-Dimitrova et al, 2023., Ocampos et al, 2023. C. alpina remains a species that has received limited investigated attention and as a result, there were few reports to compare the distribution of its metabolites with.…”

Section: Discussionmentioning

confidence: 99%

“…Significant variability was observed not only within individual samples of the same accessions but also among the eight distinct populations analyzed. As a result, numerous chemotypes and genotypes varying in their phytochemical profile were characterized for their potent AH activity and other antioxidant properties that have emerged (Ocampos et al, 2023;Zheleva-Dimitrova et al, 2023). Further investigations are mandatory to explore these promising plant species on the chemical and genetic level not only to establish breeding programs aimed at domesticating this species but also to get a better understanding of the underlying adaptation process during evolution enabling the plant to grow at various altitudes and environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies support the assumption that certain STLs themselves or in synergy with other natural substances are responsible for the AH activity of plants and plant extracts (Valente et al, 2021;Ranasinghe et al, 2023b). For this reason, special attention was given not only to the main STL derivative (GPL), but also to LAC, LACP, and other known insecticidal metabolites, such as ChA, CGA, and 3.5 DCQ during the chemical analyses of C. alpina (Valente et al, 2021;Ocampos et al, 2023). According to the analysis, the eight accessions were all rich in ChA, GPL, CGA, 3.5 DCQ, and caftaric acid.…”

Section: Stls and Caffeic Acid Derivativesmentioning

confidence: 99%

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Assessment of the chemical and genetic variability among accessions of Cicerbita alpina (L.) Wallr., an alpine plant with anthelmintic properties

Martinidou,

Palmieri,

Sordo

et al. 2023

Front. Plant Sci.

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Cicerbita alpina (L.) Wallr, is a perennial alpine plant and a member of the Asteraceae family, typically found at altitudes above 1000 meters in the Italian Alps. Although previously utilized primarily as a local delicacy, recent studies have revealed strong antiparasitic activity through in vitro experiments. In Europe, numerous chemical drugs employed to combat nematodes — helminths that infest the digestive tract of livestock — are banned due to their environmental harm or show only reduced efficiency because of the development of resistance. Consequently, there is a growing demand for new alternative anthelmintic treatments in agricultural practices. Specialized metabolites found in the extracts of C. alpina could offer a sustainable and biological alternative to chemical drugs, specifically for nematode control. For this purpose, a unique germplasm collection originating from eight distinct natural populations in the Italian Alps was analyzed for its chemical diversity using state-of-the-art targeted LC-MS/MS spectrometry, including quantification based on multiple reaction monitoring. The predominant metabolites identified from the species were the caffeic acid derivatives chicoric acid, chlorogenic acid, and 3. 5-dicaffeoylquinic acid, the sesquiterpene lactone derivative 8-O-acetyl-15-ß-D-glucopyranosyl lactucin and the flavone glycosides, apigenin-7-O-glucoside and luteolin-7-O-glucoside, alongside their precursors apigenin and luteolin, respectively. Additionally, the genetic diversity of eighty individual plants within the germplasm collection was evaluated using ten DNA molecular markers (Simple Sequence Repeats), successfully transferred from two closely related species (Cichorium intybus and Tanacetum parthenium). This investigation unveiled a significant range of genetic diversity within the examined populations, resulting in the establishment of three distinct genetic groups. The findings were further correlated with the original ecological environment and local climate conditions spanning a biennial period, indicating substantial variations among the different accessions and the intricate interplay between genetic background and environmental factors. These results could serve as a basis for future domestication of the species through plant breeding programs ensuring product quality, but also facilitating the cultivation of C. alpina in more diverse geographic regions.

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Section: Polyphenolic Contentsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 88%