Two insecticidal tetranortriterpenoids from Azadirachta indica

Siddiqui, Bina Shaheen; Afshan, Farhana; Ghiasuddin,; Faizi, Shaheen; Naqvi, S. N. H.; Tariq, Rana Muhammad Sabir

doi:10.1016/s0031-9422(99)00548-8

Cited by 84 publications

(55 citation statements)

References 7 publications

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“…The monoterpene isolated from oil produced by Tagetes minuta was active at concentrations above 40ppm 10 , and the diterpene isolated from Melantheria albinervia showed an LC 100 of 62.5ppm 24 . Furthermore, a triterpene isolated from Azadirachta indica showed an LC 50 of 21ppm 17 . All of these concentrations were much higher than those observed for fractions of C. reticulata oil-resin.…”

Section: Resultsmentioning

confidence: 99%

Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae)

Silva

Geris

Filho

et al. 2007

Rev. Soc. Bras. Med. Trop.

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Section: Resultsmentioning

confidence: 99%

Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae)

Silva

Geris

Filho

et al. 2007

Rev. Soc. Bras. Med. Trop.

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“…7). [34]. Furthermore, in 1 H-NMR spectral widths are quite narrow, so that this condition is easily fulfilled.…”

Section: H Nmr Spectramentioning

confidence: 97%

RETRACTED ARTICLE: Biological and Biochemical Potential of Sea Snake Venom and Characterization of Phospholipase A2 and Anticoagulation Activity

et al. 2015

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This study is designed to isolate and purify a novel anti-clotting protein component from the venom of Enhydrina schistosa, and explore its biochemical and biological activities. The active protein was purified from the venom of E. schistosa by ion-exchange chromatography using DEAE-cellulose. The venom protein was tested by various parameters such as, proteolytic, haemolytic, phospholipase and anti-coagulant activities. 80 % purity was obtained in the final stage of purification and the purity level of venom was revealed as a single protein band of about 44 kDa in SDS-polyacrylamide electrophoresis under reducing conditions. The results showed that the Potent hemolytic activity was observed against cow, goat, chicken and human (A, B and O positive) erythrocytes. Furthermore, the clotting assays showed that the venom of E. schistosa significantly prolonged in activated partial thromboplastin time, thrombin time, and prothrombin time. Venomous enzymes which hydrolyzed casein and gelatin substrate were found in this venom protein. Gelatinolytic activity was optimal at pH 5-9 and 1 H NMR analysis of purified venom was the base line information for the structural determination. These results suggested that the E. schistosa venom holds good promise for the development of novel lead compounds for pharmacological applications in near future.

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“…Rukmini and Raychaudhuri, (1991) have reported that seeds of A. indica have oil containing 50% oleic and 15% linoleic acids. Siddiqui et al (2000) isolated two new triterpenoids, 6 alpha-O-acetyl-7-deacetylnimocinol and meliacinol from the methanolic extract of fresh leaves of A. indica.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, Sangwan and Sangwan (1998) have reported extracts of fresh leaves of Melia azedarach to kill H. contortus larvae in vitro. However, Dakshinkar et al (1997) The parts and forms of A. indica used by various workers include seeds, leaves, and bark (Gowda, 1997;Jani et al, 1997;Mostofa et al, 1996;Rekha et al, 1997), as a paste (Rekha et al, 1997), decoction (Jani et al, 1997), aqueous extracts (Gowda, 1997;Udeinya, 1993), methanol (Udeinya, 1993;Siddiqui et al, 2000), acetone and dimethylsulfoxoide extracts (Udeinya, 1993) and oils (Rahman, 1975).…”

Section: Introductionmentioning

confidence: 99%

In vivo and in vitro antihelmintic activity of gemmotherapeutically treated Azadirachta indica (neem) against gastrointestinal nematodes of sheep and earthworms

Dogar¹,

Aslam²,

Khalil-ur-Rahman³

et al. 2012

Afr. J. Pharm. Pharmacol.

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In vivo and in vitro crude powder (CPE), native (NNE) and gemotherapeutic (GNE) extracts ofAzadirachta indica (neem) were studied to rationalize its traditional use. Live Haemonchus contortus and earthworms were used to access the in vitro and anthelmintic effect of crude extracts (NNE and GNE) of neem. The in vitro inhibitory effect of both GNE and NNE was evident in the paralysis or mortality of H. contortus and earthworms noted at 24 h post exposure. In egg hatch assay, NNE extract demonstrated inhibitory effect on egg hatching of H. Contortus after 48 h, with 100% eggs remaining unhatched at 4.0 mg/mL; while in GNE after 48 h, again 100% eggs remained unhatched at 2.0 mg/mL. All these results were also compared with positive control velbazine. For in vivo studies, GTNE, NNE and crude powder of neem were administered in increasing doses (1.0-3.0 g/kg) to sheep naturally infected with mixed species of gastrointestinal nematodes. A maximum reduction of 73.6% EPG was recorded in sheep treated with GNE at 3.0 g followed by crude powder of neem at 3.0 g (27%) and NNE at 3.0 g (43.8%). Levamisole, a standard anthelmintic agent, showed 99.23% reduction in faecal eggs per gram (EPG) basis. The data show that GNE and NNE exhibit dose dependent anthelmintic activity both in vivo and in vitro. The results suggest that utilization of GTNE, NNE and crude powder of neem may be useful in the control of sheep gastrointestinal nematodes.

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Two insecticidal tetranortriterpenoids from Azadirachta indica

Cited by 84 publications

References 7 publications

Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae)

Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae)

RETRACTED ARTICLE: Biological and Biochemical Potential of Sea Snake Venom and Characterization of Phospholipase A2 and Anticoagulation Activity

In vivo and in vitro antihelmintic activity of gemmotherapeutically treated Azadirachta indica (neem) against gastrointestinal nematodes of sheep and earthworms

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