Two mechanisms underlying the loss of p16Ink4a function are associated with distinct tumorigenic consequences for WS MEFs escaping from senescence

Wu, Xiaoming; Jia, Shuting; Zhang, Xiufeng; Si, Xiaoyu; Tang, Wenru; Luo, Ying

doi:10.1016/j.mad.2012.07.002

Cited by 13 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, RC-6 induced some cells to give rise to cellular senescence. Previous studies indicated that p16, p21 and p27 are related to cellular senescence in senescence cells (49)(50)(51)(52)(53). Therefore, these proteins were determined in the present study.…”

Section: Senescence Characteristics In Nt2 Cells After Rc-6 Treatmentmentioning

confidence: 88%

“…Previous studies indicated that β-galactosidase activity is a major characteristic found in senescence cells and is generally applied to senescence detection (65)(66)(67). In addition, many studies have reported that p16 and p21 protein levels are increased in senescence cells (50)(51)(52)(53). At present, a small fraction of RC-6-treated NT2 cells can express β-galactosidase activity.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

et al. 2014

View full text Add to dashboard Cite

Abstract.Frog ribonucleases have been demonstrated to have anticancer activities. However, whether RC-6 ribonuclease exerts anticancer activity on human embryonal carcinoma cells remains unclear. In the present study, RC-6 induced cytotoxicity in NT2 cells (a human embryonal carcinoma cell line) and our studies showed that RC-6 can exert anticancer effects and induce caspase-9 and -3 activities. Moreover, to date, there is no evidence that frog ribonuclease-induced cytotoxicity effects are related to cellular senescence. Therefore, our studies showed that RC-6 can increase p16 and p21 protein levels and induce cellular senescence in NT2 cells. Notably, similar to retinoic acid-differentiated NT2 cells, neuron-like morphology was found on some remaining live cells after RC-6 treatment. In conclusion, our study is the first to demonstrate that RC-6 can induce cytotoxic effects, caspase-9/-3 activities, cellular senescence and neuron-like morphology in NT2 cells.

show abstract

Section: Senescence Characteristics In Nt2 Cells After Rc-6 Treatmentmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In summary, our current study demonstrates the clinicopathological significance of the p16-cyclin D1-Rb pathway and the MIB-1 level in skull base chordoma and chondrosarcoma. We believe that therapies targeting the cell cycle regulation, by exogenous over-expression of p16, 20 , 24 or introduction of the anti-sense oligonucleotides of cyclin D1, 25 or interference of Rb activity, may prove valuable in the treatment of cancer. 26 In addition, the molecular mechanisms involved in the Rb pathway (CDK4/6 pathway) and tumorigenesis of skull base tumors are being researched.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological significance of p16, cyclin D1, Rb and MIB‐1 levels in skull base chordoma and chondrosarcoma

Liu

Zhang

Wang

2015

World j. otorhinolaryngol.-head neck surg.

View full text Add to dashboard Cite

ObjectiveTo investigate the expression of p16, cyclin D1, retinoblastoma tumor suppressor protein (Rb) and MIB-1 in skull base chordoma and chondrosarcoma tissues, and to determine the clinicopathological significance of the above indexes in these diseases.MethodsA total of 100 skull base chordoma, 30 chondrosarcoma, and 20 normal cartilage tissue samples were analyzed by immunohistochemistry. The expression levels of p16, cyclinD1, Rb and MIB-1 proteins were assessed for potential correlation with the clinicopathological features.ResultsAs compared to normal cartilage specimen (control), there was decreased expression of p16, and increased expression of cyclin D1, Rb and MIB-1 proteins, in both skull base chordoma and chondrosarcoma specimens. MIB-1 LI levels were significantly increased in skull base chordoma specimens with negative expression of p16, and positive expression of cyclin D1 and Rb (P < 0.05). Significantly elevated MIB-1 LI was also detected in skull base chondrosarcoma tissues, while there was negative expression of p16, cyclin D1 and Rb (P < 0.05). In skull base chordoma, p16 negatively correlated with cyclin D1 and Rb, while cyclin D1 positively correlated with Rb. Additionally, p16, cyclin D1, Rb, or MIB-1 expression showed no correlation with age, gender, or pathological classification of patients with skull base chordoma (P > 0.05). However, p16 and MIB-1 levels correlated with the intradural invasion, and expression of p16, Rb and MIB-1 correlated with the number of tumor foci (P < 0.05). Further, the expression of p16 and MIB-1 appeared to correlate with the prognosis of patients with skull base chordoma.ConclusionsThe abnormal expression of p16, cyclin D1 and Rb proteins might be associated with the tumorigenesis of skull base chordoma and chondrosarcoma.

show abstract

“…Senescent cells are prone to escape senescence by disrupting the function of the DDR pathway, mainly by mutating the p53 gene or other checkpoint regulatory genes. For example, when mouse WS embryonic fibroblast cells senesce, the cells are prone to escape senescence and re‐enter the cell cycle by either mutating p53 or deleting the genomic fragment of the Ink4A locus [Jia et al, ; Wu et al, ]. These studies have revealed a key role of the p53 gene status and DDR pathway in the surveillance of abnormal proliferation and utilizing cellular senescence against tumorigenesis.…”

Section: Crosstalk In the Molecular Pathway Regulating Aging And Tumomentioning

confidence: 99%

“…The time point and the status of cells for interference might be the key for determining the consequent longevity or tumor growth. Another possible strategy for reducing the stress of the cellular surveillance system was reducing the expression level of p16Ink4a while not affecting p53 [Wu et al, ].…”

Section: Crosstalk In the Molecular Pathway Regulating Aging And Tumomentioning

confidence: 99%

Balancing Between Aging and Cancer: Molecular Genetics Meets Traditional Chinese Medicine

et al. 2017

Self Cite

View full text Add to dashboard Cite

The biological consequences of cellular senescence and immortalization in aging and cancer are in conflict. Organisms have developed common cellular signaling pathways and surveillance mechanisms to control the processing of aging against tumorigenesis. The imbalance of any signals involved in this process may result in either premature aging or tumorigenesis and reduce the life span of the organism. In contrast, the balance between aging and tumorigenesis at a higher level (homeostatic-balance) may benefit the organism with tumor-free longevity. The focus of this perspective is to review the literature on the balance between "Yin" and "Yang" in traditional Chinese medicine. Modern cellular and molecular techniques now permit a more robust system to screen herbs in traditional Chinese medicine for their activity in balancing aging and tumorigenesis. The understanding of the crosstalk between aging and tumorigenesis and new perspectives on the application of Chinese medicine might shed light on anti-aging and tumor-free strategies. J. Cell. Biochem. 118: 2581-2586, 2017. © 2017 Wiley Periodicals, Inc.

show abstract

Two mechanisms underlying the loss of p16Ink4a function are associated with distinct tumorigenic consequences for WS MEFs escaping from senescence

Cited by 13 publications

References 30 publications

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

Clinicopathological significance of p16, cyclin D1, Rb and MIB‐1 levels in skull base chordoma and chondrosarcoma

Balancing Between Aging and Cancer: Molecular Genetics Meets Traditional Chinese Medicine

Contact Info

Product

Resources

About