Two modes of Na extrusion in cells from guinea pig kidney cortex slices

Whittembury, Guillermo; Proverbio, Fulgencio

doi:10.1007/bf00587893

Cited by 199 publications

(89 citation statements)

References 52 publications

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“…Finally, it is possible that sodium is transported by renal tubular cells in at least two different ways, one dependent on Na-K-ATPase and the other independent of it, and Whittembury has adduced evidence from experiments with tissue slices in support of this concept (37,38). The present results would then suggest the hypothesis that the Na-K-ATPase-dependent or ouabain sensitive pathway predominates in the ascending limb and distal tubules, while a ouabain-insensitive pathway is prominent in the proximal tubule.…”

Section: Methodsmentioning

confidence: 99%

The distribution of sodium-potassium-activated adenosine triphosphatase in medulla and cortex of the kidney

Hendler¹,

Torretti²,

Epstein³

1971

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

The distribution of sodium-potassium-activated adenosine triphosphatase in medulla and cortex of the kidney

Hendler¹,

Torretti²,

Epstein³

1971

J. Clin. Invest.

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“…The activity of the NaCl pump appears to be directly or indirectly (e.g. via Ca2+) stimulated by a rise in acinar cell Na concentration and is inhibited by the diuretic agent ethacrynic acid in both the kidney (Whittembury & Proverbio, 1970) and the salivary gland (Petersen, 1970). Nerve stimulation increases the permeability of the acinar cells to K which diffuses out ofthe cell allowing Na to enter along its electrochemical gradient, thus stimulating salivation.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous salivation in the rabbit submandibular gland

Smaje¹

1973

The Journal of Physiology

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summABY 1. Salivation has been studied in the submandibular gland of the rabbit. A very slow spontaneous salivation took place when all possibility of nerve influence had been excluded. Salivation was not due to ultrafiltration.2. The 'spontaneous' saliva had a mean K concentration of 148 mM and Na concentration of 46 mm. With increasing salivation rate produced by parasympathetic nerve stimulation, K concentration fell to a plateau level of about 30 mm whilst Na concentration fell rapidly to reach the low value of 3 mM, then began to rise again at the higher flow rates.3. Ligation of the submandibular duct produced a reversal of the ion concentrations in spontaneous saliva. By 4 days K concentration was lower and that of Na higher than control values until by 2 weeks the effect was maximal with mean concentrations of 25 mm for K and 153 mm for Na.4. Ouabain increased the spontaneous salivation rate and ethacrynic acid slowed or prevented it altogether. On the basis of the known sites of action of these drugs it is postulated that two pumps are involved in the regulation of spontaneous salivation. There appears to be basal activity of an acinar mechanism pumping NaCl into the lumen, taking water with it. This pump is activated directly or indirectly by the intracellular Na concentration which itself is controlled by an Na-K exchange pump.5. Excitation of the sympathetic trunk produced a small, though definite, increase in salivation rate. There was evidence that myoepithelial cells might also be involved in the sympathetic response and that they were activated by a receptor stimulation. Salivation evoked by sympathetic nerve stimulation would seem to be a response to , receptor stimulation, but the possibility that activation of both ar and ft receptors was required could not be excluded entirely.

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“…The slices were dried on filter paper, weighed, desiccated for 24 h at 100°C, and reweighed. They were then shaken for 24 h in 2 ml 1 N HNO3, and the Na +, K ÷ and C1-concentrations of the resulting extracts were determined [4]. Fig.…”

Section: Methodsmentioning

confidence: 99%

“…This active Na ÷ extrusion occurs via two mechanisms: Na ÷ extrusion in exchange for K ÷ and Na ÷ extrusion along with C1-and water [1][2][3][4][5]. A ouabain-sensitive, Na,K-stimulated ATPase activity, responsible for the Na ÷ extrusion in exchange for K +, and a ouabain-insensitive, Na-stimulated ATPase activity, responsible for the Na ÷ extrusion along with C1-and water, have been demonstrated in basolateral plasma membranes of proximal tubular ceils from guinea-pig and rat kidney [6][7][8]11].…”

Section: Introductionmentioning

confidence: 99%

Effect of a high Na⁺ diet on cell volume and Na⁺‐stimulated ATPase activities of rat kidney membranes

1990

FEBS Letters

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Proximal tubular cells from kidneys of male rats chronically fed with an isotonic NaC1 solution, show a volume increase which is dependent on the length of the treatment with NaC1, when compared with control rats. Parallel to the cell volume increase, there is an increase of the ouabaininsensitive Na-ATPase activity, whereas the ouabain-sensitive Na,K-ATPase activity remains unchanged. These results establish a clear relationship between a chronic Na-diet, kidney cell volume and Na-ATPase activity.

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Two modes of Na extrusion in cells from guinea pig kidney cortex slices

Cited by 199 publications

References 52 publications

The distribution of sodium-potassium-activated adenosine triphosphatase in medulla and cortex of the kidney

The distribution of sodium-potassium-activated adenosine triphosphatase in medulla and cortex of the kidney

Spontaneous salivation in the rabbit submandibular gland

Effect of a high Na⁺ diet on cell volume and Na⁺‐stimulated ATPase activities of rat kidney membranes

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Two modes of Na extrusion in cells from guinea pig kidney cortex slices

Cited by 199 publications

References 52 publications

The distribution of sodium-potassium-activated adenosine triphosphatase in medulla and cortex of the kidney

The distribution of sodium-potassium-activated adenosine triphosphatase in medulla and cortex of the kidney

Spontaneous salivation in the rabbit submandibular gland

Effect of a high Na+ diet on cell volume and Na+‐stimulated ATPase activities of rat kidney membranes

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Product

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Effect of a high Na⁺ diet on cell volume and Na⁺‐stimulated ATPase activities of rat kidney membranes