Two new catechins from Zijuan green tea enhance the fitness and lifespan of <i>Caenorhabditis elegans via</i> insulin-like signaling pathways

Ke, Jia-Ping; Yu, Jing-Ya; Gao, Biao; Hu, Fenglin; Xu, Fengqing; Yao, Guangmin; Bao, Guan‐Hu

doi:10.1039/d2fo01795d

Cited by 13 publications

(5 citation statements)

References 52 publications

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“…In this study, the higher concentrations of astaxanthin were less effective for increasing the lifespan of C. elegans . Similar dose-response effects on lifespan were found for other bioactive compounds [ 61 , 62 ]. It is well established that both nutrients and beneficial/toxic chemicals may produce opposite consequences for an organism depending on the doses.…”

Section: Discussionsupporting

confidence: 75%

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

Ding

Zhao

2022

Antioxidants

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Astaxanthin is a marine xanthophyll carotenoid which effectively prevents intracellular oxidative stress and has beneficial effects against various human diseases. It has been shown that astaxanthin protects Caenorhabditis elegans (C. elegans) from oxidative damages and extends the lifespan of C. elegans possibly by modulating genes involved in insulin/insulin-like growth factor (IGF) signaling (IIS) and the oxidoreductase system, although the exact mechanisms remain elusive. In this study, RNA sequencing analyses were employed to identify the differentially expressed genes in C. elegans in response to astaxanthin treatment. A total of 190 mRNAs and 6 microRNAs (miRNAs) were significantly changed by astaxanthin treatment in C. elegans. Gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that the mRNAs and miRNAs significantly altered by astaxanthin mainly function in innate immunity, lipid metabolism and stress responses, a significant portion of which are related to lifespan regulation in C. elegans. The study revealed novel mRNA and miRNA targets of astaxanthin, providing new insights for understanding the anti-aging mechanisms and the biological function of astaxanthin.

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Section: Discussionsupporting

confidence: 75%

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

Ding

Zhao

2022

Antioxidants

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“…elegans within 20 s were measured at 6, 24, and 48 h after heating. Five nematodes were selected from each medium for counting, with three parallel readings of each worm …”

Section: Methodsmentioning

confidence: 99%

“…Five nematodes were selected from each medium for counting, with three parallel readings of each worm. 36 CL4176 Strain Neurotoxicity Assay. The synchronized L3-stage CL4176 strain was transferred to NGM plates containing inactivated OP50 and corresponding compounds (myricetin, quercetin, 1, 2, 3, and 4 at 100 μM, with 0.1% DMSO set as the control group), cultured at 16 °C for 3 days.…”

Section: Synthesis and Isolationmentioning

confidence: 99%

Novel Flavonol Alkaloids in Green Tea: Synthesis, Detection, and Anti-Alzheimer’s Disease Effect in a Transgenic Caenorhabditis elegans CL4176 Model

Chen,

Yang,

et al. 2024

J. Agric. Food Chem.

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Novel N-ethy-2-pyrrolidinone-substituted flavonols, myricetin alkaloids A−C (1−3), quercetin alkaloids A−C (4a, 4b, and 5), and kaempferol alkaloids A and B (6 and 7), were prepared from thermal reaction products of myricetin, quercetin, kaempferol�L-theanine, respectively. We used HPLC-ESI-HRMS/MS to detect 1−7 in 14 cultivars of green tea and found that they were all present in "Shuchazao," "Longjing 43", "Fudingdabai", and "Zhongcha 108" green teas. The structures of 1−4 and 6 were determined by extensive 1D and 2D NMR spectroscopies. These flavonol alkaloids along with their skeletal flavonols were assessed for anti-Alzheimer's disease effect based on molecular docking, acetylcholinesterase inhibition, and the transgenic Caenorhabditis elegans CL4176 model. Compound 7 strongly binds to the protein amyloid β (Aβ 1−42 ) through hydrogen bonds (BE: −9.5 kcal/mol, K i : 114.3 nM). Compound 3 (100 μM) is the strongest one in significantly extending the mean lifespan (13.4 ± 0.5 d, 43.0% promotion), delaying the Aβ 1−42 -induced paralysis (PT 50 : 40.7 ± 1.9 h, 17.1% promotion), enhancing the locomotion (140.0% promotion at 48 h), and alleviating glutamic acid (Glu)-induced neurotoxicity (153.5% promotion at 48 h) of CL4176 worms (p < 0.0001).

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“…The lifespan of nematodes was measured using the methods of Sutphin et al [45]. Briefly, on day 0, synchronized L1 larvae were added to NGM plates containing different drugs and cultured.…”

Section: Lifespan Experimentsmentioning

confidence: 99%

Epigallocatechin-3-Gallate Reduces Cd-Induced Developmental Toxicity of Bodysize in Caenorhabditis elegans via the PEK-1/eIF-2α/ATF-4 Pathway

Wang,

Chen,

2023

Molecules

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Cadmium (Cd), a harmful heavy metal that has no biological purpose, can harm healthy fetal and child development. Epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in tea, has been shown to increase cell viability under Cd exposure and ameliorate Cd-induced kidney injury in adult male rats. Using the Caenorhabditis elegans (C. elegans) model, we demonstrated that EGCG mitigated Cd-induced body size developmental toxicity through a mechanism that did not involve chelation with EGCG and was not associated with Cd accumulation and efflux. Our research indicated that the beneficial effects of EGCG on Cd-induced body size developmental toxicity were associated with the mitigation of endoplasmic reticulum stress. Furthermore, our observations indicate that EGCG reduced Cd-induced developmental toxicity in C. elegans via the PEK-1/eIF-2α/ATF-4 pathway. Our results provide important evidence for the potential benefits of consuming tea as a detoxification agent.

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Two new catechins from Zijuan green tea enhance the fitness and lifespan of Caenorhabditis elegans via insulin-like signaling pathways

Abstract: Green tea polyphenols show positive effects on human health and longevity. However, knowledge of the anti-aging properties of green tea is limited to that of the major catechin epigallocatechin gallate...

Cited by 13 publications

References 52 publications

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

Astaxanthin Induces Transcriptomic Responses Associated with Lifespan Extension in Caenorhabditis elegans

Novel Flavonol Alkaloids in Green Tea: Synthesis, Detection, and Anti-Alzheimer’s Disease Effect in a Transgenic Caenorhabditis elegans CL4176 Model

Epigallocatechin-3-Gallate Reduces Cd-Induced Developmental Toxicity of Bodysize in Caenorhabditis elegans via the PEK-1/eIF-2α/ATF-4 Pathway

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