Two New Compounds from the Leaves of Calocedrus microlepic var. formosana.

Abstract

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Three New Diterpenoids from the Leaves ofThuja orientalis

“…The IR spectrum indicated the presence of a carboxylic carbonyl (1691 cm −1 ) and an olefin (3078, 1641 cm −1 ) group. The 1 H and 13 C-NMR data (l " Tables 1 and 2) were very similar to those reported for 15-methoxypinusolidic acid [9] and 2. By comparison with the NMR data of 15-methoxypinusolidic acid, the major differences were the disappearance of the carbonyl carbon signal (δ C 171.3) and the presence of the methine carbon (δ C 107.1), methoxy carbon (δ C 54.6), and methine proton (δ H 5.53) signals.…”

“…The relative stereochemistry was assumed to be the same as that of Table 1 and 2) were quite similar to those reported for 15-methoxypinusolidic acid, which was isolated from Calocedrus microlepic var. formosana [9]. The major differences were the absence of the methoxy carbon and proton signals (δ C 56.9; δ H 3.55, respectively) and the presence of n-butoxy carbon 2S).…”

“…The purification of the n-hexane-soluble fraction led to the isolation of three new diterpenoids (1-3) (l " Fig. 1) and twelve known compounds (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15), which were identified on the basis of spectroscopic analyses. The isolated compounds were tested for cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cancer cell lines using the sulforhodamin B (SRB) bioassay.…”

“…The major differences were the absence of the methoxy carbon and proton signals (δ C 56.9; δ H 3.55, respectively) and the presence of n-butoxy carbon 2S). The relative stereochemistry was assumed to be the same as that of 15-methoxypinusolidic acid [9] based on the J values. The NOESY correlations confirmed the stereochemistry (Fig.…”

“…3S). The relative stereochemistry was assumed to be the same as that of 15-methoxypinusolidic acid [9] based on the J values. The ROESY correlations of H-5/H-9 and H-11/H-20 confirmed the stereochemistry (Fig.…”