Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations

Abstract

Tylopilus himalayanus and T. pseudoballoui are described as new species from two Himalayan states (Sikkim and Uttarakhand) in India. Tylopilus himalayanus is characterised by a unique combination of features: reddish- or brownish-grey to purplish-grey then brown to reddish-brown or darker pileus, absence of olive or violet tinges on stipe surface, angular pores, stipe without reticulum or rarely with a faint reticulum restricted to the very apex, bitter taste of the context and positive macrochemical colour reaction of the stipe context with KOH (dark orange) and FeSO4 (dark green), medium sized (10.9–14.4 × 3.9–4.9 µm) basidiospores and occurrence under coniferous trees; T. pseudoballoui is distinguished by orange-yellow to brown-yellow sticky pileus, pale yellow pore surface with pinkish hues that turns pale to greyish-orange on bruising; angular pores, stipe concolorous to pileus with pruinose but never reticulate surface, ixocutis pattern of pileipellis and occurrence under broadleaf trees. Another species, T. neofelleus, which was reported earlier from China and Japan, was also collected from Sikkim and reported for the first time from India. All three species are described with morphological details and two-locus based (nrLSU and nrITS) phylogenetic data.

Tylopilus nigripes sp. nov. (Boletaceae, Basidiomycota) from the Atlantic Forest of Brazil

“…Tylopilus nigripes is keyed into Boletaceae based in the following features (Wu et al 2016): (1) hymenophoral tubes are not reddish, pinkish red, red or reddish brown and pores regularly arranged; (2) hymenophoral tube never becoming yellow (3) smooth basidiospores; (4) stipe base never bright or chrome yellow; (5) glabrous stipe surface; (6) pileipellis not a ixotrichoderm; (7) basidiome without distinct membranous veil; and (8) context pale vinaceous when cut. Although the bitter taste was not observed in the moment of the collection, all other features fit our new species in Tylopilus sensu Wu et al (2016), Magnago et al (2017), Chakraborty et al (2018), Liang et al (2018) and Gelardi et al (2019).…”

“…Tylopilus exiguus T.W. Henkel from Guyana clearly belongs to this genus according to Chakraborty et al (2018) and Gelardi et al (2019), and is similar to T. nigripes in its small size (pileus 10–30 mm in diam. ), and the black stipe.…”

Inocybe mytiliodora: A New Record for Turkey

“…nrLSU (nuclear ribosomal large subunit) is a widely used DNA barcoding marker, which is useful for the identification of fungal samples at the species level (Xiao et al, 2018). As the data of morphological traits is not sufficient alone for precise identification of fungi species, the use of sequence data from conserved DNA regions such as nrITS and nrLSU is considered to be an important tool for researchers with expertise in taxonomy and systematics of fungi since the last three decades (Vellinga, 2004;Chakraborty, Vizzini & Das, 2018;Xiao et al, 2018).…”

Tylopilus glutinosus, a new species of Boletaceae from Bangladesh

“…The taxa included in the phylogenetic analyses were mostly selected based on previous molecular phylogenetic studies on the Boletaceae (Wu et al 2014, Gelardi et al 2015, Chakraborty et al 2018), and blast search results against the NCBI database. Initially, the newly obtained nrLSU sequences from the new bolete were used as a query in a blast search to compare with those already deposited in GenBank.…”