Two Novel Mouse Genes—Nubp2, Mapped to the t-Complex on Chromosome 17, and Nubp1, Mapped to Chromosome 16— Establish a New Gene Family of Nucleotide-Binding Proteins in Eukaryotes

Nakashima, Hiroshi; Grahovac, Marija J.; Mazzarella, Richard; Fujiwara, Hiroyuki; Kitchen, John R.; Threat, Tracy A.; Ko, Minoru S.H.

doi:10.1006/geno.1999.5898

Cited by 19 publications

(15 citation statements)

References 37 publications

(68 reference statements)

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“…A database search revealed that this positive clone corresponded to 'nucleotide-binding protein 2' (Nubp2, Fig. 7B), an ATPase of the NUBP/MRP subfamily (Nakashima et al, 1999). Mouse Nubp2 has extensive sequence similarity with prokaryotic protein MinD, a member of the NifH-ArsA-Par-MinD subgroup of type A ATPases (Walker et al, 1982;Koonin, 1993).…”

Section: Identification Of Kifc5a From Mouse Hippocampusmentioning

confidence: 99%

“…The important role of MinD in the accurate placement of the cell division machinery in prokaryotes (FtsZ ring), as well as its intriguing behaviour as a 'motor protein in prokaryotes' (Sakai et al, 2001), made the protein identified in our screen an attractive candidate for further study. Two mouse proteins with sequence similarity to MinD have been described so far: Nubp1 has a unique N-terminal extension containing four cysteine residues and Nubp2 is a shorter form without the N-terminal extension (Nakashima et al, 1999) (Fig. 7B).…”

Section: Identification Of Kifc5a From Mouse Hippocampusmentioning

confidence: 99%

“…7B). Nubp1 and Nubp2 are encoded by genes on different chromosomes and share a characteristic P-loop motif and two highly conserved NUBP/MRP motifs ␣ and ␤ (Nakashima et al, 1999) (Fig. 7B).…”

Section: Identification Of Kifc5a From Mouse Hippocampusmentioning

confidence: 99%

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Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication

Christodoulou

Lederer

Surrey

et al. 2006

Journal of Cell Science

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Inhibition of motor protein activity has been linked with defects in the formation of poles in the spindle of dividing cells. However, the molecular mechanisms underlying the functional relationship between motor activity and centrosome dynamics have remained uncharacterised. Here, we characterise KIFC5A, a mouse kinesin-like protein that is highly expressed in dividing cells and tissues, and is subject to developmental and cell-type-specific regulation. KIFC5A is a minus-end-directed, microtubule-dependent motor that produces velocities of up to 1.26 μm minute-1 in gliding assays and possesses microtubule bundling activity. It is nuclear in interphase, localises to the centre of the two microtubule asters at the beginning of mitosis, and to spindle microtubules in later mitotic phases. Overexpression of KIFC5A in mouse cells causes the formation of aberrant, non-separated microtubule asters and mitotic arrest in a prometaphase-like state. KIFC5A knockdown partly rescues the phenotype caused by inhibition of plus-end-directed motor Eg5 by monastrol on the mitotic spindle, indicating that it is involved in the balance of forces determining bipolar spindle assembly and integrity. Silencing of KIFC5A also results in centrosome amplification detectable throughout the cell cycle. Supernumerary centrosomes arise primarily as a result of reduplication and partly as a result of cytokinesis defects. They contain duplicated centrioles and have the ability to organise microtubule asters, resulting in the formation of multipolar spindles. We show that KIFC5A interacts with nucleotide-binding proteins 1 and 2 (Nubp1 and Nubp2), which have extensive sequence similarity to prokaryotic division-site-determining protein MinD. Nubp1 and Nubp2 also interact with each other. Knockdown of Nubp1 or double knockdown of Nubp1 and Nubp2 (Nubp1&Nubp2) both phenocopy the KIFC5A silencing effect. These results implicate KIFC5A and the Nubp proteins in a common regulatory pathway involved in the control of centrosome duplication in mammalian cells.

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Section: Identification Of Kifc5a From Mouse Hippocampusmentioning

confidence: 99%

Section: Identification Of Kifc5a From Mouse Hippocampusmentioning

confidence: 99%

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Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication

Christodoulou

Lederer

Surrey

et al. 2006

Journal of Cell Science

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“…Definitive identification of genes was based on BLAST searches (Altschul et al 1997), which revealed five genes homologous to the published genes: Rsp29, Igfals, Nubp2, Ndk3, and Jsap1 (Ji et al 1997;Boisclair et al 1996;Nakashima et al 1999;Venturelli et al 1995;Ito et al 1999). The search also revealed six putative transcripts identified by homology to ESTs.…”

Section: Global Analysismentioning

confidence: 99%

“…The CpG island contains the entire coding sequence of the first exon (including the initiation codon and the first part of the open reading frame). It is transcribed from the reverse strand, with the 3Ј end of the gene very near to the 3Ј end of the Igfals gene (between coordinates 19982 and 23657), and has been further characterized as defining a new subfamily of nucleotide binding proteins (Nakashima et al 1999). The genomic sequence and cDNA show a translation start site followed by seven exons (exons 2 and 3 strongly predicted by GRAIL) and a stop codon, with a tail of 400 bp of 3Ј untranslated sequence containing the polyadenylation signal (Nakashima et al 1999).…”

Section: Nucleotide Binding Protein 2 (Nubp2)mentioning

confidence: 99%

Eleven Densely Clustered Genes, Six of them Novel, in 176 kb of Mouse t-complex DNA

et al. 2000

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Targeted sequencing of the mouse t-complex has started with a 176-kb, gene-rich BAC localized with six PCR-based markers in inversion 2/3 of the highly duplicated region. The sequence contains 11 genes recovered primarily as cDNAs from early embryonic collections, including Igfals (previously placed on chromosome 17),Nubp2 (a fully characterized gene), Jsap1 (a JNK-binding protein), Rsp29 (the mouse homologue of the rat gene), Ndk3 (a nucleoside diphosphate kinase), and six additional putative genes of unknown function. With 50% GC content, 75% of the DNA transcribed, and one gene/16.0 kb (on average), the region may qualify as one of the most gene-dense segments in the mouse genome and provides candidates for dosage-sensitive phenotypes and mouse embryonic lethals mapped to the vicinity.[The sequence data described in this paper have been submitted to the GenBank data library under accession no. AF220294.]

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Current Awareness on Comparative and Functional Genomics

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Two Novel Mouse Genes—Nubp2, Mapped to the t-Complex on Chromosome 17, and Nubp1, Mapped to Chromosome 16— Establish a New Gene Family of Nucleotide-Binding Proteins in Eukaryotes

Cited by 19 publications

References 37 publications

Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication

Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication

Eleven Densely Clustered Genes, Six of them Novel, in 176 kb of Mouse t-complex DNA

Current Awareness on Comparative and Functional Genomics

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