Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.)

Ye, Rongjian; Zhou, Fei; Lin, Yongjun

doi:10.1007/s00299-012-1238-8

Cited by 57 publications

(64 citation statements)

References 48 publications

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“…Double asterisk means difference is significant at the 0.01 level unintended impacts on plant physiology, the tissue or organ specific promoters with diverse specificities were used for a precise spatial control of EβF expression in rice. For example, utilizing the strong rice green tissue-specific promoters, such as rbcS (small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase) promoter (Huang and Lin 2007;Ye et al 2009) and DX1 promoter (Ye et al 2012), facilitated highly active expression of EβFS only in green tissues, but not in non-green tissues. In addition, using wounding or insectfeeding inducible promoters, such as maize proteinase inhibitor (mpi) promoter (Cordero et al 1994;Quilis et al 2014) and Pib (Wang et al 2001;Yu et al 2010), would let the plant produce EβF only after feeding by pests.…”

Section: Discussionmentioning

confidence: 99%

Expression of a Peppermint (E)-β-Farnesene Synthase Gene in Rice Has Significant Repelling Effect on Bird Cherry-Oat Aphid (Rhopalosiphum padi)

et al. 2015

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Aphids (Aphididae) cause great damage to economically important cereal crops due to their unparalleled reproductive capacity. The sesquiterpene (E)-β-farnesene (EβF) is the alarm pheromone for many aphids, which can be applied to disperse aphids in crops. The peppermint EβF synthase gene (EβFS), which enables the production of EβF in plants to control aphids, has been used in Arabidopsis and tobacco, but has not been studied in rice or other crops yet. Here, we transformed the peppermint EβFS gene into the rice variety Zhonghua 11 and obtained two homozygous transgenic lines with single-copy insertion. EβFS expression and EβF production were then verified by reverse transcription-PCR (RT-PCR)/quantitative real-time PCR (qPCR) and gas chromatography-mass spectrometry (GC-MS). Although only a limited amount of EβF was detected, the transgenic lines showed a significant repelling effect on bird cherry-oat aphid (Rhopalosiphum padi) compared with the control. Thus, this study provides a valuable reference for improving the resistance against aphids in rice or other important crops.

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Section: Discussionmentioning

confidence: 99%

Expression of a Peppermint (E)-β-Farnesene Synthase Gene in Rice Has Significant Repelling Effect on Bird Cherry-Oat Aphid (Rhopalosiphum padi)

et al. 2015

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“…The 5′ deletions of the OsAAP6 promoter from ZS97 and NYZ at positions −1,814, −1,226, −698 and −377 were generated by PCR amplification and inserted into expression vector pDX2181 (ref. 52). The resulting constructs (designated as −1,814, −1,226, −698 and −377 from ZS97 and NYZ4, respectively) were introduced into Agrobacterium tumefaciens strain EHA105 and transferred into ZH11 by Agrobacterium- mediated transformation50.…”

Section: Methodsmentioning

confidence: 99%

“…GUS activity in the various tissues of transgenic-positive plants was localized histochemically52, and photographs were taken under a dissecting microscope. Tissues of transgenic plants were incubated overnight in GUS staining solution at 37 °C, dehydrated and embedded in paraffin (Sigma).…”

Section: Methodsmentioning

confidence: 99%

OsAAP6 functions as an important regulator of grain protein content and nutritional quality in rice

et al. 2014

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Grains from cereals contribute an important source of protein to human food, and grain protein content (GPC) is an important determinant of nutritional quality in cereals. Here we show that the quantitative trait locus (QTL) qPC1 in rice controls GPC by regulating the synthesis and accumulation of glutelins, prolamins, globulins, albumins and starch. qPC1 encodes a putative amino acid transporter OsAAP6, which functions as a positive regulator of GPC in rice, such that higher expression of OsAAP6 is correlated with higher GPC. OsAAP6 greatly enhances root absorption of a range of amino acids and has effects on the distribution of various amino acids. Two common variations in the potential cis-regulatory elements of the OsAAP6 5′-untranslated region seem to be associated with GPC diversity mainly in indica cultivars. Our results represent the first step toward unravelling the mechanism of regulation underlying natural variation of GPC in rice.

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“…Many promoter elements, such as the ASF-1 motif or activation sequence-1 (as-1; TGACG), OSE1 (AAAGAT), OSE2 (CTCTT), and TAPOX1 (ATATT), were found to be involved in root-specific expression in plants (Dey and Maiti 1999;Elmayan and Tepfer 1995;Vijaybhaskar et al 2008). In contrast, an ATATT-containing sequence was expected to be responsible for the green tissue-specific expression of DX1, which is a rice gene (Ye et al 2012).…”

Section: Introductionmentioning

confidence: 95%

A Region Containing an as-1 Element of Dahlia Mosaic Virus (DaMV) Subgenomic Transcript Promoter Plays a Key Role in Green Tissue- and Root-Specific Expression in Plants

et al. 2014

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A subgenomic transcript (Sgt) promoter was isolated from the genomic clone of dahlia mosaic virus (DaMV), which is a double-stranded DNA virus of the Caulimoviridae family. The DaMVSgt promoter, which is linked to the heterologous β-glucuronidase (GUS) reporter gene, was characterized in transient protoplasts and in transgenic tobacco, as well as in Arabidopsis plants. The 5′-and 3′-deletion analysis of a 591-bp DaMVSgt promoter fragment indicated that a 441-bp promoter fragment (−372 to +69 from the transcription start site; TSS) was sufficient for maximal promoter activity. A 141-bp promoter fragment (−72 to +69 from TSS) was the minimal promoter region that also showed relatively strong activity. The three activation sequence-1 (as-1) elements and the border regions were primarily responsible for the promoter activity, as revealed by a finer internal deletion and mutation analysis of the cis-elements and of the immediate border sequence of the activation domain. Electrophoretic mobility shift assay (EMSA), supershift EMSA, DNase I footprinting, Southwestern blotting, and UV cross-linking studies demonstrated the binding of a tobacco transcription factor, TGA1a, that correlated with 2,4-dichlorophenylacetic acid (2,4D)-induced transcriptional activity of the DaMVSgt promoter. Histological GUS staining and the GUS enzymatic assay demonstrated that the 441-bp DaMVSgt4 promoter and 141-bp minimal DaMVSgt4F are 5.5 and 4.6 times, respectively, stronger than the CaMV 35S promoter. The minimal DaMVSgt4F promoter is more active than CaMV 35S in all types of green tissues and roots, without any detectable expression in reproductive tissues and seeds. The DaMVSgt4F promoter may be useful for transgene containment applications.

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Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.)

Abstract: We have isolated and characterized the rice green tissue-specific promoter P(DX1), and identified two novel positive cis-acting elements in P(DX1).

Cited by 57 publications

References 48 publications

Expression of a Peppermint (E)-β-Farnesene Synthase Gene in Rice Has Significant Repelling Effect on Bird Cherry-Oat Aphid (Rhopalosiphum padi)

Expression of a Peppermint (E)-β-Farnesene Synthase Gene in Rice Has Significant Repelling Effect on Bird Cherry-Oat Aphid (Rhopalosiphum padi)

OsAAP6 functions as an important regulator of grain protein content and nutritional quality in rice

A Region Containing an as-1 Element of Dahlia Mosaic Virus (DaMV) Subgenomic Transcript Promoter Plays a Key Role in Green Tissue- and Root-Specific Expression in Plants

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