Two novel solvent system compositions for protected synthetic peptide purification by centrifugal partition chromatography

Amarouche, Nassima; Giraud, Matthieu; Forni, Luciano; Butté, Alessandro; Edwards, F.; Borie, Nicolas; Renault, Jean‐Hugues

doi:10.1016/j.chroma.2014.02.052

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…CPC processing is based on the solutes separated in different distribution coefficients ( Kd ) between two immiscible solvent systems and the ratio of solvent system is adjusted to get the Kd value of target analyte between 0.5 and 2.0 [ 33 ]. In this study, the Kd values of TC and cis -crocetin were performed in four solvent systems as shown in the Table 4 .…”

Section: Resultsmentioning

confidence: 99%

High-Purity Preparation of Enzyme Transformed Trans-Crocetin Reclaimed from Gardenia Fruit Waste

Chyau

Chiu

Hsieh

et al. 2022

Plants

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The recovery of physiologically bioactive ingredients from agricultural wastes as an abundant and low-cost source for the production of high value-added mutraceuticlas has been recognized and supported for the commercial interests and sustainable managements. In the extraction of geniposide for the development of natural food colorants from the dried fruits of Gardenia jasminoides Rubiaceae, the gardenia fruit waste (GFW) still remaining 0.86% (w/w) of crocins has always been discarded without any further treatments Until now, there was no simple and effective protocol for high-purity trans-crocein (TC) preparation without the coexistence of non-biologically active cis-crocein from GFW. We proposed an effective process to obtain the compound as follows. Crocins were extracted firstly by 50% of ethanol in the highest yield of 8.61 mg/g (w/w) from GFW. After the HPD-100 column fractionation in the collecting of crocins, the conversion ratio of 75% of crocins to crocetins can be obtained from the commercial available enzyme- Celluclast® 1.5 L. The crocins hydrolyzed products, were then separated through the HPD-100 resin adsorption and finally purified with the centrifugal partition chromatography (CPC) in single-step to obtain TC in a purity of 96.76 ± 0.17%. Conclusively, the effective enzyme transformation and purification co-operated with CPC technologies on crocins resulted in a high purity product of TC may be highly application in the commercial production.

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Section: Resultsmentioning

confidence: 99%

High-Purity Preparation of Enzyme Transformed Trans-Crocetin Reclaimed from Gardenia Fruit Waste

Chyau

Chiu

Hsieh

et al. 2022

Plants

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“…As for now, two most popular scales of biphasic solvent systems are as follows: The ARIZONA systems range (or HEMWat), composed of heptane (or hexane), ethyl acetate, methanol, and water, with the modification as OKA scale, with n ‐butanol as a fifth solvent (also called “extended ARIZONA”). The acetone scale composed of heptane, toluene, acetone, and water . Two novel solvent system compositions for protected synthetic peptide purification by CPC were proposed by Amarouche et al. . Protected synthetic peptide intermediates are often hydrophobic and not soluble in most common solvents.…”

Section: Improvements and Modifications Of The Centrifugal Partition mentioning

confidence: 99%

“…Two novel solvent system compositions for protected synthetic peptide purification by CPC were proposed by Amarouche et al. . Protected synthetic peptide intermediates are often hydrophobic and not soluble in most common solvents.…”

Section: Improvements and Modifications Of The Centrifugal Partition mentioning

confidence: 99%

Centrifugal partition chromatography - A review of recent applications and some classic references

Bojczuk

Żyżelewicz

Hodurek³

2017

J. Sep. Sci.

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Countercurrent chromatography, based on liquid-liquid partitioning, has many technological variants. One of them is centrifugal partition chromatography, introduced by Wataru Murayama and Kanichi Nunogaki in 1982. This technique, like other countercurrent chromatography techniques, is based on the phenomenon of liquid-liquid partitioning between two immiscible liquid phases that stay at equilibrium. But the significant difference between this technique and others is the retention mechanism of stationary phase. In the case of centrifugal partition chromatography, this mechanism is based on hydrostatic force, formed by the centrifugal field in the rotor in one-axis centrifuge. Sometimes that allows more control of stationary phase, for example, when aqueous two-phase and other difficult solvent systems are used. However, the efficiency of the separation in centrifugal partition chromatography is also affected by a variety of parameters dependent on the sample properties in the solvent system, physical properties of the solvent system, parameters of the instrument, and the method. This article includes also recent ideas for improvements to the technique and broadening its application (e.g., (multiple) dual-mode or elution-extrusion procedure, pH-zone-refining centrifugal partition chromatography, ion-exchange centrifugal partition chromatography, online and offline coupling of centrifugal partition chromatography).

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“…CPC has two main elution modes: descending mode where the less dense “upper” phase is stationary and the denser “lower” phase is pumped through it, and ascending mode where the lower phase is stationary and the upper phase is pumped through it . CPC has been utilized for many separations including that of natural products, proteins and peptides, − and most recently for the enantioseparation of chiral compounds …”

Section: Introductionmentioning

confidence: 99%

Continuous Separation and Purification of Chemical Species by Continuous Liquid–Liquid Partition Chromatography Implemented with Membrane-Based Phase Separators

Viano,

Milani,

Orkenyi

et al. 2023

Org. Process Res. Dev.

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New equipment and methodology for the continuous separation and purification of chemical species via continuous liquid–liquid partition chromatography are presented. This is achieved by cascading countercurrent mixing and extraction steps carried out with slug flow in membrane-based phase separators. This methodology provides a continuous implementation of liquid–liquid separations typically achieved with centrifugal partition chromatography while eliminating several mechanical drawbacks and limitations and provides an alternative to simulated moving bed chromatography without the need for a solid phase. We present the methodology, describe and validate a predictive mathematical model to determine optimal operating conditions, and give example separations including chiral separation and scale up.

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Two novel solvent system compositions for protected synthetic peptide purification by centrifugal partition chromatography

Cited by 7 publications

References 27 publications

High-Purity Preparation of Enzyme Transformed Trans-Crocetin Reclaimed from Gardenia Fruit Waste

High-Purity Preparation of Enzyme Transformed Trans-Crocetin Reclaimed from Gardenia Fruit Waste

Centrifugal partition chromatography - A review of recent applications and some classic references

Continuous Separation and Purification of Chemical Species by Continuous Liquid–Liquid Partition Chromatography Implemented with Membrane-Based Phase Separators

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