Two pathogenic species of Pythium: P. aphanidermatum and P. diclinum from a wheat field

Al-Sheikh, Hashem

doi:10.1016/j.sjbs.2010.05.001

Cited by 13 publications

(10 citation statements)

References 9 publications

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“…Traditional methods of studying the population of Pythium and Fusarium species have involved culture plating technique onto a selective medium (Al-Sheikh, 2010;Banks et al, 1996;Cha et al, 2007) and the observation of morphological features. However, the accuracy and reliability of these techniques depends on the experience and skill of the person making the diagnosis (McCartney, Foster, Fraaije, & Ward, 2003).…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of polyclonal antibodies for detection of Pythium aphanidermatum and Fusarium oxysporum in ginger

Ray

Dash

Achary

et al. 2017

Food and Agricultural Immunology

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Polyclonal antibodies against Pythium aphanidermatum and Fusarium oxysporum proteins were developed for the detection of rhizome rot in ginger using serological assays. Under optimal experimental conditions, the detection limit of P. aphanidermatum by indirect ELISA was 10 µg/ml with a linear working range from 5 to 100 µg/ml (R 2 = 0.994). In case of F. oxysporum, the linear working range was 5-100 µg/ml (R 2 = 0.991) and the limit of detection was 25 µg/ml. The developed antibodies showed the highest titer in ELISA at 1:2000 dilutions. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis identified proteins ranging from molecular weights 15-97 kDa and 14-116 kDa of P. aphanidermatum and F. oxysporum isolates, respectively. In Western blot analysis, the developed antisera gave positive reactions against the isolated antigens of the fungi. The antibodies revealed immune-reactive bands of molecular weights 59 and 65 kDa in P. aphanidermatum and 44 and 75 kDa in F. oxysporum. The results suggest that the developed antibodies could be successfully applied for the specific immunodetection of P. aphanidermatum and F. oxysporum at an early stage of rhizome rot disease. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Development and evaluation of polyclonal antibodies for detection of Pythium aphanidermatum and Fusarium oxysporum in ginger

Ray

Dash

Achary

et al. 2017

Food and Agricultural Immunology

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“…The Pa isolate was from diseased tomatoes isolated from the Disease Diagnosis Clinic at Utah State University. Attack of wheat by P. aphanidermatum isolates is documented to occur under field conditions (Al-Sheikh 2010). Accordingly, inoculation of wheat or tomato seed in laboratory tests with either of these isolates eliminated seedling stand (Anderson et al unpublished data).…”

Section: Introductionmentioning

confidence: 99%

Pesticidal activity of metal oxide nanoparticles on plant pathogenic isolates of Pythium

et al. 2015

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CuO and ZnO nanoparticles (NPs) have antimicrobial effects that could lead to formulations as pesticides for agriculture or medicine. The responses of two soil-borne plant pathogenic Pythium isolates to the NPs were studied to determine the potential of these metal oxide NPs as pesticides. Growth of the P. ultimum isolate was more sensitive to CuO NPs than the P. aphanidermatum isolate. Growth in liquid medium with CuO NPs eliminated culturability whereas exposure to ZnO NPs resulted in stasis with growth resuming on transfer to medium lacking NPs. The citrate in the medium used for the growth assays was involved in enhanced release of the toxic metals from the NPs. Both CuO and ZnO NPs affected processes involved in Fe uptake. The NPs reduced levels of Fe-chelating siderophore-like metabolites produced by Pythium hyphae. CuO NPs inhibited, but ZnO NPs increased, ferric reductase activity detected at the mycelial surface. These findings illustrate that the toxicity of the metal oxide NPs towards Pythium was influenced by the medium, especially by the presence of a metal chelator. Environmental factors are likely to alter the pesticide potential of the metal oxide NPs when formulated for agricultural use in soils.

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“…Several control measures of the disease are available, including fungicidal control, enhancement of genetic resistance, which may lead to deteriorating human health [5,20]. In Egypt, there is no active control method to reduce damping-off of many crops and vegetable plants caused by P. aphanidermatum although, that pathogenic is widely distributed throughout the country [17,18,21,22]. However, fungicides are regularly used by farmers to manage such diseases without obvious results [6,23].…”

Section: Discussionmentioning

confidence: 99%

“…P. oligandrum used in biocontrol of black scurf in potato as the hyphal of biocontrol agent had colonized the sclerotia and established close contact by coiling around the R. solani [17]. Phytium oligandrum has taken serious attention as a biocontrol agent against damping-off diseases, especially those caused by P. aphanidermatum [8,16,18,19].…”

Section: Introductionmentioning

confidence: 99%

Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

Sayed

Abdelmohsen

Abdelzaher

et al. 2021

Plants

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The role of Pythium oligandrum as a biocontrol agent against Pythium aphanidermatum was investigated to avoid the harmful impacts of fungicides. Three isolates of P. oligandrum (MS15, MS19, and MS31) were assessed facing the plant pathogenic P. aphanidermatum the causal agent of Glycine max damping-off. The tested Pythium species were recognized according to their cultural and microscopic characterizations. The identification was confirmed through sequencing of rDNA-ITS regions including the 5.8 S rDNA. The biocontrol agent, P. oligandrum, isolates decreased the mycelial growth of the pathogenic P. aphanidermatum with 71.3%, 67.1%, and 68.7% through mycoparasitism on CMA plates. While the half-strength millipore sterilized filtrates of P. oligandrum isolates degrade the pathogenic mycelial linear growth by 34.1%, 32.5%, and 31.7%, and reduce the mycelial dry weight of the pathogenic P. aphanidermatum by 40.1%, 37.4%, and 36.8%, respectively. Scanning electron microscopy (SEM) of the most effective antagonistic P. oligandrum isolate (MS15) interaction showed coiling, haustorial parts of P. oligandrum to P. aphanidermatum hyphae. Furthermore, P. oligandrum isolates were proven to enhance the germination of Glycine max seedling to 93.3% in damping-off infection using agar pots and promote germination of up to 80% during soil pot assay. On the other hand, P. oligandrum isolates increase the shoot, root lengths, and the number of lateral roots.

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Two pathogenic species of Pythium: P. aphanidermatum and P. diclinum from a wheat field

Cited by 13 publications

References 9 publications

Development and evaluation of polyclonal antibodies for detection of Pythium aphanidermatum and Fusarium oxysporum in ginger

Development and evaluation of polyclonal antibodies for detection of Pythium aphanidermatum and Fusarium oxysporum in ginger

Pesticidal activity of metal oxide nanoparticles on plant pathogenic isolates of Pythium

Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

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