Two patterns of cytokine production by placental macrophages

Павлов, О. В.; Selutin, A.V.; Pavlova, Oksana; Selkov, S. A.

doi:10.1016/j.placenta.2020.01.005

Cited by 19 publications

(16 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The production of TGF-β1/β2 and IL-1RA is in accordance with HBCs’ M2 homeostatic and tolerogenic activities ( 26 , 30 , 83 , 84 ). In agreement with our findings, third-trimester HBCs produce high levels of IL-8 ( 29 , 30 , 85 , 86 ) and were identified, together with trophoblasts, as responsible for the constitutive production of IL-8 in the human placenta ( 122 ). Thus, under our experimental conditions, HBCs retain their in vivo profile of cytokine production.…”

Section: Discussionsupporting

confidence: 90%

Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors

Azari

Johnson

Webb

et al. 2021

mBio

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors

Azari

Johnson

Webb

et al. 2021

mBio

View full text Add to dashboard Cite

show abstract

“…Previous studies have yielded variable findings concerning the phenotype, cytokine secretion, and functions of HBCs ( Johnson and Chakraborty, 2012 ; Young et al, 2015 ; Pavlov et al, 2020 ; Loegl et al, 2016 ; Schliefsteiner et al, 2017 ; Swieboda et al, 2020 ), probably due to the failure to account for PAMM contamination, which we show is present in placental digests. Here, using our gating strategy for the isolation of placental myeloid cell populations, we find that steady-state HBCs secrete a range of factors that play a role in vascularization and the remodeling of blood vessels, such as VEGF-A, OPN, MMP-9, and TIMP-1 ( Johnson et al, 2003 ; Dai et al, 2009 ; Poggio et al, 2011 ; Luizon et al, 2014 ; Plaks et al, 2013 ).…”

Section: Discussionmentioning

confidence: 54%

“…The work presented in this study is limited to first-trimester samples. Previous studies have investigated the properties of HBCs across gestation ( Goldstein et al, 1988 ; Ingman et al, 2010 ; Swieboda et al, 2020 ; Pavlov et al, 2020 ). However, studies that used placental digests have not considered contamination with PAMM populations, and so interpretation of some of their findings is difficult.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and functional characterization of first-trimester human placental macrophages, Hofbauer cells

Thomas

Appios

Zhao

et al. 2020

Journal of Experimental Medicine

124

166

View full text Add to dashboard Cite

Hofbauer cells (HBCs) are a population of macrophages found in high abundance within the stroma of the first-trimester human placenta. HBCs are the only fetal immune cell population within the stroma of healthy placenta. However, the functional properties of these cells are poorly described. Aligning with their predicted origin via primitive hematopoiesis, we find that HBCs are transcriptionally similar to yolk sac macrophages. Phenotypically, HBCs can be identified as HLA-DR−FOLR2+ macrophages. We identify a number of factors that HBCs secrete (including OPN and MMP-9) that could affect placental angiogenesis and remodeling. We determine that HBCs have the capacity to play a defensive role, where they are responsive to Toll-like receptor stimulation and are microbicidal. Finally, we also identify a population of placenta-associated maternal macrophages (PAMM1a) that adhere to the placental surface and express factors, such as fibronectin, that may aid in repair.

show abstract

“…Previous studies have yielded variable findings concerning the phenotype, cytokine secretion and functions of HBC(Johnson and Chakraborty, 2012; Young et al, 2015; Pavlov et al, 2020; Loegl et al, 2016; Schliefsteiner et al, 2017; Swieboda et al, 2020), probably due to the failure to account for PAMM contamination which we show is present in placental digests. Here, using our gating strategy for the isolation of placental myeloid cell populations we find that steady-state HBC secrete a range of factors that play a role in vascularisation and the remodelling of blood vessels, such as VEGF-A, OPN, MMP-9 and TIMP-1 (Johnson et al, 2003; Dai et al, 2009; Poggio et al, 2011; Luizon et al, 2014; Plaks et al, 2013).…”

Section: Discussionmentioning

confidence: 54%

“…The work presented in this study is limited to first trimester samples. Previous studies have investigated the properties of HBC across gestation(Goldstein et al, 1988; Ingman et al, 2010; Swieboda et al, 2020; Pavlov et al, 2020). However, studies that used placental digests have not considered contamination with PAMM populations and so interpretation of some of their findings is difficult.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and functional characterisation of first trimester human placental macrophages, Hofbauer cells

Thomas

Appios

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Hofbauer cells (HBC) are a population of macrophages found in high abundance within the stroma of the first trimester human placenta. HBC are the only fetal immune cell population within the stroma of healthy placenta. However, the functional properties of these cells are poorly described. Aligning with their predicted origin via primitive haematopoiesis, we find that HBC are transcriptionally similar to yolk sac macrophages. Phenotypically, HBC can be identified as HLA-DR-FOLR2+ macrophages. We identify a number of factors HBC secrete (including IL-8 and MMP-9) that could affect placental angiogenesis and remodelling. We determine that HBC have the capacity to play a defensive role, where they are responsive to Toll-like receptor stimulation and are microbicidal. Finally, we also identify a population of placenta-associated maternal macrophages (PAMM1a) that adhere to the placental surface and express factors, such as fibronectin, that may aid in repair.SummaryUsing transcriptomic and proteomic data, Thomas, J. et al, analyse human first trimester placental macrophages and delineate markers that identify them. They also reveal that Hofbauer cells have microbicidal capacity, providing the fetus with an additional layer of protection from certain microbes.One sentence summaryHofbauer cells are primitive placental macrophages with a unique phenotype and role in fetal defence.Non-standard abbreviationPlacental associated maternal monocytes/macrophages, PAMM.

show abstract

Two patterns of cytokine production by placental macrophages

Cited by 19 publications

References 75 publications

Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors

Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors

Phenotypic and functional characterization of first-trimester human placental macrophages, Hofbauer cells

Phenotypic and functional characterisation of first trimester human placental macrophages, Hofbauer cells

Contact Info

Product

Resources

About