Two-phase dual-signal-readout immunosensing platform based on multifunctional carbon nano-onions for ovarian cancer biomarker detection

Huang, Yitian; Zhang, Shupei; Chen, Yanjie; Gao, Li; Dai, Hong; Lin, Yanyu

doi:10.1039/d3tb00824j

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…The analysis of actual samples is significant to prove the practicability of the biosensor, − and clinical serum samples from Xinyang Central Hospital were tested. The serum samples were used directly without any dilution.…”

Section: Resultsmentioning

confidence: 99%

In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi₄O₅I₂: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis

Xiao,

Wu,

et al. 2024

ACS Appl. Mater. Interfaces

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As one of the interesting signaling mechanisms, the in situ growth reaction on a photoelectrode has proven its powerful potential in photoelectrochemical (PEC) bioanalysis. However, the specific interaction between the signaling species with the photoactive materials limits the general application of the signal mechanism. Herein, on the basis of an in situ growth reaction on a photoelectrode of single-atom-based photoactive material, a general PEC immunoassay was developed in a split-type mode consisting of the immunoreaction and PEC detection procedure. Specifically, a single-atom photoactive material that incorporates Fe atoms into layered Bi 4 O 5 I 2 (Bi 4 O 5 I 2 −Fe SAs) was used as a photoelectrode for PEC detection. The sandwich immunoreaction was performed in a well of a 96-well plate using Ag nanoparticles (Ag NPs) as signal tracers. In the PEC detection procedure, the Ag + converted from Ag NPs were transferred onto the surface of the Bi 4 O 5 I 2 − Fe SAs photoelectrode and thereafter AgI was generated on the Bi 4 O 5 I 2 −Fe SAs in situ to form a heterojunction through the reaction of Ag + with Bi 4 O 5 I 2 −Fe SAs. The formation of heterojunction greatly promoted the electro-hole separation, boosting the photocurrent response. Exemplified by myoglobin (Myo) as the analyte, the immunosensor achieved a wide linear range from 1.0 × 10 −11 to 5.0 × 10 −8 g mL −1 with a detection limit of 3.5 × 10 −12 g mL −1 . This strategy provides a general PEC immunoassay for disease-related proteins, as well as extends the application scope of in situ growth reaction in PEC analysis.

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Section: Resultsmentioning

confidence: 99%

In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi₄O₅I₂: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis

Xiao,

Wu,

et al. 2024

ACS Appl. Mater. Interfaces

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The Promise of Carbon Nano‐Onions: Preparation, Characterization and Their Application in Electrochemical Sensing

Almeida Gonzalez,

Hernandez Ojeda,

Corcho‐Valdés

et al. 2024

Analysis & Sensing

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Carbon nano‐onions (CNOs) promise to improve the range of applications of carbon materials for electroanalytical applications. In this review we explore the synthesis, characterization, and electrochemical applications of CNOs. CNOs‐based sensors present impressive features, including low detection limits in the femtogram per milliliter range, a wide linear detection range spanning up to 7 orders of magnitude, exceptional selectivity, reproducibility, and stability. Synthetic methods and characterization techniques for CNOs were thoroughly examined, shedding light on their pivotal role in biosensing technologies. Comparative analyses with other carbon materials underscore CNOs' competitive performance, either surpassing or matching many counterparts. Despite their relatively recent integration in biosensing applications, CNOs exhibit comparable or superior results concerning other carbon‐based materials. Indeed, the incorporation of CNOs into hybrid nanocomposites have shown promising outcomes, indicating a synergistic potential for future advancements in biosensing technologies. Our review provides a broad approach of the application of CNOs to the field, with emphasis in breakthroughs of the last 5 years.

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Overview of the Design and Application of Photothermal Immunoassays

Gao,

Wu,

Gan

et al. 2024

Sensors

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Developing powerful immunoassays for sensitive and real-time detection of targets has always been a challenging task. Due to their advantages of direct readout, controllable sensing, and low background interference, photothermal immunoassays have become a type of new technology that can be used for various applications such as disease diagnosis, environmental monitoring, and food safety. By modification with antibodies, photothermal materials can induce temperature changes by converting light energy into heat, thereby reporting specific target recognition events. This article reviews the design and application of photothermal immunoassays based on different photothermal materials, including noble metal nanomaterials, carbon-based nanomaterials, two-dimensional nanomaterials, metal oxide and sulfide nanomaterials, Prussian blue nanoparticles, small organic molecules, polymers, etc. It pays special attention to the role of photothermal materials and the working principle of various immunoassays. Additionally, the challenges and prospects for future development of photothermal immunoassays are briefly discussed.

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Two-phase dual-signal-readout immunosensing platform based on multifunctional carbon nano-onions for ovarian cancer biomarker detection

Abstract: In order to detect early tumors without symptoms and gain valuable time for treatment, there is an urgent need to develop a fast, cheap, and ultrasensitive multi-reading detection platform for...

Cited by 3 publications

References 43 publications

In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi₄O₅I₂: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis

In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi₄O₅I₂: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis

The Promise of Carbon Nano‐Onions: Preparation, Characterization and Their Application in Electrochemical Sensing

Overview of the Design and Application of Photothermal Immunoassays

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Two-phase dual-signal-readout immunosensing platform based on multifunctional carbon nano-onions for ovarian cancer biomarker detection

Abstract: In order to detect early tumors without symptoms and gain valuable time for treatment, there is an urgent need to develop a fast, cheap, and ultrasensitive multi-reading detection platform for...

Cited by 3 publications

References 43 publications

In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi4O5I2: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis

In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi4O5I2: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis

The Promise of Carbon Nano‐Onions: Preparation, Characterization and Their Application in Electrochemical Sensing

Overview of the Design and Application of Photothermal Immunoassays

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Product

Resources

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In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi₄O₅I₂: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis

In Situ Growth Reaction on Photoelectrodes of Single-Atom Fe Incorporated Bi₄O₅I₂: A General Photoelectrochemical Immunoassay Toward Sensitive Protein Analysis