Two-photon absorption of 7-hydroxycoumarine

Parma, L.; Omenetto, N.

doi:10.1016/0009-2614(78)85280-4

Cited by 17 publications

(11 citation statements)

References 7 publications

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“…The two-photon absorption of dye solutions at elevated laser intensities has been studied previously by fluorescence observation [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Two-photon absorption cross-sections G {2) were determined for various dyes [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

S0-S n two-photon absorption dynamics of rhodamine dyes

Sperber

Penzkofer

1986

Opt Quant Electron

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The intensity-dependent transmission of picosecond ruby laser pulses of different duration through methanolic and ethanolic solutions of rhodamine B and rhodamine 6G is analysed. The transmission is affected by S 0 -S" two-photon absorption, by stimulated emission at the pumplaser frequency, by amplified spontaneous emission and by excited-state absorption. Various parameters involving the two-photon absorption dynamics are determined by comparing experiments with numerical simulations.

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Section: Introductionmentioning

confidence: 99%

S0-S n two-photon absorption dynamics of rhodamine dyes

Sperber

Penzkofer

1986

Opt Quant Electron

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“…) is quite crude and because Parma & Omenetto (19) have claimed that Q f1S is only 0.32Q f2S for the closely related 7-hydroxycoumarin. Therefore, the absolute magnitudes of the cross sections here are probably reliable to no better than a factor of two, although their relative magnitudes may be more accurate.…”

mentioning

confidence: 99%

Brominated 7-hydroxycoumarin-4-ylmethyls: Photolabile protecting groups with biologically useful cross-sections for two photon photolysis

Furuta

Wang

Dantzker

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

611

589

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Photochemical release (uncaging) of bioactive messengers with three-dimensional spatial resolution in light-scattering media would be greatly facilitated if the photolysis could be powered by pairs of IR photons rather than the customary single UV photons. The quadratic dependence on light intensity would confine the photolysis to the focus point of the laser, and the longer wavelengths would be much less affected by scattering. However, previous caged messengers have had very small cross sections for two-photon excitation in the IR region. We now show that brominated 7-hydroxycoumarin-4-ylmethyl esters and carbamates efficiently release carboxylates and amines on photolysis, with one-and two-photon cross sections up to one or two orders of magnitude better than previously available. These advantages are demonstrated on neurons in brain slices from rat cortex and hippocampus excited by glutamate uncaged from N-(6-bromo-7-hydroxycoumarin-4-ylmethoxycarbonyl)-L-glutamate (Bhc-glu). Conventional UV photolysis of Bhc-glu requires less than one-fifth the intensities needed by one of the best previous caged glutamates, ␥-(␣-carboxy-2-nitrobenzyl)-L-glutamate (CNB-glu). Two-photon photolysis with rasterscanned femtosecond IR pulses gives the first threedimensionally resolved maps of the glutamate sensitivity of neurons in intact slices. Bhc-glu and analogs should allow more efficient and three-dimensionally localized uncaging and photocleavage, not only in cell biology and neurobiology but also in many technological applications.

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“…Within the S 0 state the population of levels 7, 8, 9 thermalize with vibrational relaxation time T v [16][17][18]. The fluorescence emission within the S 0 -S { absorption region is partially reabsorbed (transition 7 3). The pump laser at frequency v L and the generated amplified spontaneous emission signal at frequency v ASE suffer excited-state absorption from S, to S w2 and S n3 , respectively.…”

Section: Theorymentioning

confidence: 99%

“…Two-photon absorption cross-sections G {2) were determined for various dyes [1][2][3][4][5][6][7]. The fluorescence induced by two-photon absorption has found wide application in picosecond-pulse duration measurements [8].…”

Section: Introductionmentioning

confidence: 99%