Two-Photon Fluorescence Microscopy and Applications in Angiogenesis and Related Molecular Events

Lee, Marcus; Kannan, Sathya; Muniraj, Giridharan; Rosa, Vinícius; Lu, Wen Feng; Fuh, Jerry Ying Hsi; Sriram, Gopu; Cao, Tong

doi:10.1089/ten.teb.2021.0140

Cited by 9 publications

(4 citation statements)

References 109 publications

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“…To that end, various 3D imaging and noninvasive modalities like confocal laser scanning, confocal reflectance, two/multiphoton and second harmonic generation, confocal Raman spectroscopy, and light sheet microscopy have been utilized in clinical and experimental dermatology (Figure 10A-E). [88,[232][233][242][243][244][245][246][247][248][249] These imaging tools have provided an opportunity for label-free and noninvasive imaging of cellular and matrix components in 3D space that could be referred to as optical biopsy, in contrast to 2D histological images. These imaging technologies have been applied in oral mucosal biology, such as the use of two/multiphoton microscopy to visualize the keratinocytes, fibroblasts and collagen fibers (Figure 10A,B); [88,124,250] confocal reflectance microscopy to visualize fibroblasts, vasculature and extracellular matrix components within the gingival connective tissue equivalents (Figure 10C); [92,123] and light sheet microscopy to visualize vascular networks within the human gingiva (Figure 10E).…”

Section: Insights From Biofabrication Of Skin Tissue Constructsmentioning

confidence: 99%

Biofabrication Strategies for Oral Soft Tissue Regeneration

Rahimnejad,

Makkar,

Dal‐Fabbro

et al. 2024

Adv Healthcare Materials

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Gingival recession, a prevalent condition affecting the gum tissues, is characterized by the exposure of tooth root surfaces due to the displacement of the gingival margin. This review explores conventional treatments, highlighting their limitations and the quest for innovative alternatives. Importantly, it emphasizes the critical considerations in gingival tissue engineering leveraging on cells, biomaterials, and signaling factors. Successful tissue‐engineered gingival constructs hinge on strategic choices such as cell sources, scaffold design, mechanical properties, and growth factor delivery. Unveiling advancements in recent biofabrication technologies like 3D bioprinting, electrospinning, and microfluidic organ‐on‐chip systems, this review elucidates their precise control over cell arrangement, biomaterials, and signaling cues. These technologies empower the recapitulation of microphysiological features, enabling the development of gingival constructs that closely emulate the anatomical, physiological, and functional characteristics of native gingival tissues. The review explores diverse engineering strategies aiming at the biofabrication of realistic tissue‐engineered gingival grafts. Further, we highlight the parallels between the skin and gingival tissues, exploring the potential transfer of biofabrication approaches from skin tissue regeneration to gingival tissue engineering. To conclude, the exploration of innovative biofabrication technologies for gingival tissues and inspiration drawn from skin tissue engineering look forward to a transformative era in regenerative dentistry with improved clinical outcomes.This article is protected by copyright. All rights reserved

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Section: Insights From Biofabrication Of Skin Tissue Constructsmentioning

confidence: 99%

Biofabrication Strategies for Oral Soft Tissue Regeneration

Rahimnejad,

Makkar,

Dal‐Fabbro

et al. 2024

Adv Healthcare Materials

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“…Ultrashort pulse fiber lasers exhibiting ultra short time scale and high peak power have attracted attention in many fields including optical communication, material processing, biomedicine, security, and scientific research. Especially in the field of biomedicine, multiphoton microscopy (MPM) using femtosecond laser sources has become a useful method for applications such as biological and medical imaging [1][2][3][4][5][6][7]. MPM is based on the principle of nonlinear optical imaging and has the characteristics of high spatiotemporal resolution and low cell damage, which is particularly suitable for noninvasive three-dimensional detection of deep within scattered biological tissues.…”

Section: Introductionmentioning

confidence: 99%

High-power Raman soliton generation tunable from 1.76 to 1.84 µm in all-fiber polarization-maintaining erbium-doped amplifier

Xu,

Yao,

et al. 2024

Fourth International Conference on Telecommunications, Optics, and Computer Science (TOCS 2023)

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An all-fiber polarization maintaining laser system of wavelength tunable from 1.76 to 1.84 µm based on the Ramaninduced soliton self-frequency shift in an erbium-doped amplifier is demonstrated. The system only includes oscillator and two-stage amplifiers which are built entirely based on commercial silica fibers and devices. The ultra-short pulse achieved by oscillator and first amplifier stage by the method of nonlinear compression is delivered into second amplifier stage which is designed as both Raman shifter and amplifier. Within the pump power range of 16-25 W, tunable Raman soliton can be obtained with the average power gradually increases from ~180 mW to ~250 mW as the center wavelength of the soliton shifts towards the longer wavelength direction within the range of 1.76 to 1.84 μm. The system delivers Raman soliton from an entirely fiberized, fusion spliced system without any free-space optics, which can provide robust and stable soliton generation. To our knowledge, this tunable soliton source is the highest output power demonstrated within 1.7-1.8 μm wavelength range from an all-fiber polarization maintaining laser. Our experiment provides a feasible femtosecond source for multiphoton microscopy.

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“…In recent years, multiphoton microscopy (MPM) using femtosecond laser sources has become a valuable tool for biological and medical imaging. [1][2][3][4][5][6][7] MPM is a nonlinear optical imaging technique that is especially useful for non-invasive three-dimensional imaging deep within scattered biological tissues. The femtosecond laser source wavelength selection of MPM is crucial for improving the depth of multiphoton imaging.…”

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confidence: 99%

High-Power Raman Soliton Generation at 1.7 μm in All-Fiber Polarization-Maintaining Erbium-Doped Amplifier

Xu 徐,

Wang 王,

Yao 姚

et al. 2024

Chinese Phys. Lett.

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An all-fiber polarization maintaining high-power laser system operating at 1.7 µm based on the Raman-induced soliton self-frequency shifting effect is demonstrated. The entirely fiberized system is built by erbium-doped oscillator and two-stage amplifiers with polarization maintaining commercial silica fibers and devices, which can provide robust and stable soliton generation. High power soliton laser with the average of 0.28 W, the repetition rate of 42.7 MHz, and pulse duration of 515 fs is generated directly from the main amplifier. Our experiment provides a feasible method for high-power all-fiber polarization maintaining femtosecond laser generation working at 1.7 μm.

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Two-Photon Fluorescence Microscopy and Applications in Angiogenesis and Related Molecular Events

Cited by 9 publications

References 109 publications

Biofabrication Strategies for Oral Soft Tissue Regeneration

Biofabrication Strategies for Oral Soft Tissue Regeneration

High-power Raman soliton generation tunable from 1.76 to 1.84 µm in all-fiber polarization-maintaining erbium-doped amplifier

High-Power Raman Soliton Generation at 1.7 μm in All-Fiber Polarization-Maintaining Erbium-Doped Amplifier

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