Two sphingomyelin synthase homologues regulate body weight and sphingomyelin synthesis in female brown planthopper, <i>N. lugens </i>(Stål)

Shi, Xiaoxiao; Zhang, H.; Chen, Mutian; Zhang, Y.-D.; Zhu, Mu-Fei; Zhang, M.-J.; Li, F.-Q.; Wratten, Stephen D.; Zhou, Wenwu; Mao, Cungui; Zhu, Zhiliang

doi:10.1111/imb.12549

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…growth, apoptosis and differentiation; Acharya et al, 2008). Sphingomyelin has important structural and functional roles in the cell (Ramstedt and Slotte, 2002), and its concentration can affect the bodyweight of brown planthopper (Shi et al, 2019). Previous studies have indicated that B. subtilis can maintain the health and promote the growth and development of silkworm (Wang et al, 2019;Yeruva et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

Zheng

Zhu

et al. 2022

Environmental Microbiology

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The symbiotic association between bacterial symbionts and insect hosts is a complicated process that is not completely understood. Herein, we used a silkworm model to study the association between symbiotic Bacillus and lepidopteran insect by investigating the changes in intestinal microbiota and hemolymph circulating metabolites of silkworm after symbiotic Bacillus subtilis treatment. Results showed that B. subtilis can generate a variety of primary and secondary metabolites, such as B vitamins and antimicrobial compounds, to provide micronutrients and enhance the pathogen resistance of their insect host. Shifts in the relative abundance of Enterococcus, Brevibacterium, Buttiauxella, Pseudomonas, Brevundimonas and Limnobacter had significant correlations with the concentrations of differential metabolites (e.g. phospholipids and certain amino acids) in insect hemolymph. The antimicrobial compounds secreted by B. subtilis were the primary driving force for the reconstruction of intestinal microbiota. Meanwhile, the altered levels of circulating metabolites in multiple metabolic pathways were potential adaptive mechanism of insect hosts in response to the shifts of intestinal microbiota. Our findings provided concrete evidence that bacterial intestinal symbiont can alter the physiological state of insects and highlighted the importance of the compositional alterations of intestinal microbiota as a source of variation in circulating metabolites of insect hosts.

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Section: Discussionmentioning

confidence: 99%

Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

Zheng

Zhu

et al. 2022

Environmental Microbiology

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“…Regarding the lipid biosynthetic process, sphingolipids are an important class of lipids that participate in crucial events of cell proliferation, differentiation, and apoptosis in various species [ 46 ]. Recently, evidence has accumulated to show that sphingolipids have roles in the signaling pathways involved in reproduction and oocyte development of N. lugens [ 47 , 48 , 49 ]. Cyclin-dependent kinase (CDK) is a serine/threonine protein kinase family that cooperates with cyclin and is required for embryo development and cell cycle progression [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of MicroRNAs Associated with Reproduction in the Brown Planthopper, Nilaparvata lugens

Wang

Zhang

Chen

et al. 2022

IJMS

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Insects have a robust capacity to produce offspring for propagation, and the reproductive events of female insects have been achieved at the molecular and physiological levels via regulatory gene pathways. However, the roles of MicroRNAs (miRNAs) in the reproductive development of the brown planthopper (BPH), Nilaparvata lugens, remain largely unexplored. To understand the roles of miRNAs in reproductive development, miRNAs were identified by Solexa sequencing in short-winged (SW) female adults of BPH. Small RNA libraries derived from three developmental phases (1 day, 3 days, and 5 days after emergence) were constructed and sequenced. We identified 905 miRNAs, including 263 known and 642 novel miRNAs. Among them, a total of 43 miRNAs were differentially expressed in the three developmental phases, and 14,568 putative targets for 43 differentially expressed miRNAs (DEMs) were predicted by TargetScan and miRanda. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the predicted miRNA targets illustrated the putative roles for these DEMs in reproduction. The progress events were annotated, including oogenesis, lipid biosynthetic process, and related pathways such as apoptosis, ABC transporters, and amino acid metabolism. Four highly abundant DEMs (miR-9a-5p, miR-34-5p, miR-275-3p, and miR-317-3p) were further screened, and miR-34-5p was confirmed to be involved in the regulation of reproduction. Overexpression of miR-34-5p via injecting its mimics reduced fecundity and decreased Vg expression. Moreover, target genes prediction for miR-34-5p showed they might be involved in 20E signaling cascades, apoptosis, and gonadal development, including hormone receptor 4 (HR4), caspase-1 (Cp-1), and spermatogenesis-associated protein 20 (SPATA20). These findings provide a valuable resource for future studies on the role of miRNAs in BPH reproductive development.

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“…Figure S1). Shi et al, 2019 demonstrated that dsRNA-mediated knockdown of N. lugens sphingomyelin synthase-like protein 1, which is involved in sphingolipids metabolism, led to increased weight of females, but not of males, suggesting a general sex specific effect of lipid metabolism modulation in planthoppers. In C. elegans, XPO1 silencing resulted in elevated lipid accumulation and increased expression of lipases (Silvestrini et al, 2018).…”

Section: Severe Obesity Observed In Pmxpo1-knockdown Femalesmentioning

confidence: 96%

Exportin 1 is required for the reproduction and maize mosaic virus accumulation in its insect vectorPeregrinus maidis

Xavier,

Tyson,

Kerner

et al. 2023

Preprint

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Exportin 1 (XPO1) is the major karyopherin-β nuclear receptor mediating the nuclear export of hundreds of proteins and some classes of RNA and regulates several critical processes in the cell, including but not limited to, cell-cycle progression, transcription, translation, oncogenesis and longevity. Viruses have co-opted XPO1 to promote nucleocytoplasmic transport of viral proteins and RNA. Maize mosaic virus (MMV) is an Alphanucleorhabdovirus transmitted in a circulative propagative manner by the corn planthopper, Peregrinus maidis. MMV replicates in the nucleus of plant and insect hosts, and it remains unknown whether MMV co-opts P. maidis XPO1 (PmXPO1) to complete its life cycle. Because XPO1 plays multiple regulatory roles in cell functions and virus infection, we hypothesized that RNAi-mediated silencing of XPO1 would simultaneously and negatively affect MMV accumulation and insect physiology. Although PmXPO1 expression was not modulated during MMV infection, PmXPO1 knockdown negatively affected MMV accumulation in P. maidis at 12 and 15 days after microinjection. Likewise, PmXPO1 knockdown negatively affected P. maidis survival and reproduction. PmXPO1 exhibited tissue specific expression patterns with higher expression in the ovaries compared to the guts of adult females. Survival rate was significantly lower for PmXPO1 knockdown females, compared to controls, but no effect was observed for males. Adult females with PmXPO1 knockdown were heavier and had a larger abdomen compared to controls at 4, 8 and 12 days after dsRNA microinjection. Consistent with an increase in weight, glyceride content specifically and significantly increased in PmXPO1 knockdown female planthoppers. Ovary development was significantly inhibited, and mature eggs were not observed in adult females with PmXPO1 knockdown. Consistent with a major role of PmXPO1 in ovary function and egg production, oviposition and egg hatch in plants was dramatically reduced in dsRNA PmXPO1 treated insects compared with control. Altogether, these results suggest that PmXPO1 is a positive regulator of P. maidis reproduction and that it plays a proviral role in the insect vector supporting MMV infection.

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Two sphingomyelin synthase homologues regulate body weight and sphingomyelin synthesis in female brown planthopper, N. lugens (Stål)

Cited by 9 publications

References 30 publications

Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

Characterization of MicroRNAs Associated with Reproduction in the Brown Planthopper, Nilaparvata lugens

Exportin 1 is required for the reproduction and maize mosaic virus accumulation in its insect vectorPeregrinus maidis

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