“…A depletion in ancient energy stock reserves, additionally to political, economic, health and environmental issues enhances scientists to go looking for alternative sustainable fuel from available sources are coming in vogue [1][2][3][4][5]. Biodiesel is one of the most potential renewable fuels, environmental friendliness, biodegradable, non-toxic, a low air pollutant emitting fuel than the petroleum diesel and has a similar engine performance [4]. Oleaginous microorganisms have the capability to accumulate significant amounts of storage triacylglycerols, considered to be a viable and attractive substitute for vegetable oils and animal fats for biodiesel production [6,7].…”