Two-stage tuberculosis diagnostics: combining centrifugal microfluidics to detect TB infection and Inh and Rif resistance at the point of care with subsequent antibiotic resistance profiling by targeted NGS

Abstract

Globally, tuberculosis (TB) remains the deadliest bacterial infectious disease and spreading antibiotic resistances is the biggest challenge for combatting the disease. Rapid and comprehensive diagnostics including drug susceptibility testing (DST)...

Fast and on-site animal species identification in processed meat via centrifugal microfluidics and isothermal amplification

“…These methods can be integrated into microfluidic chips to automate DNA extraction as has already been demonstrated for various applications. 28–30 Other methods comprise the enrichment of DNA 31,32 or simple two step crude extraction. However, none of these approaches was developed for the analysis of meat products focusing on minimal hands on time, a short time-to-result and the use of simple standard laboratory equipment.…”